Human Treponema pallidum 11q/j isolate belongs to subsp. endemicum but contains two loci with a sequence in TP0548 and TP0488 similar to subsp. pertenue and subsp. pallidum, respectively
MIKALOVÁ, Lenka, Michal STROUHAL, Jan OPPELT, Philippe Alain GRANGE, Michel JANIER et. al.
Human Treponema pallidum 11q/j isolate belongs to subsp. endemicum but contains two loci with a sequence in TP0548 and TP0488 similar to subsp. pertenue and subsp. pallidum, respectively
Authors
MIKALOVÁ, Lenka (203 Czech Republic, belonging to the institution), Michal STROUHAL (203 Czech Republic, belonging to the institution), Jan OPPELT (203 Czech Republic, belonging to the institution), Philippe Alain GRANGE (250 France), Michel JANIER (250 France), Nadjet BENHADDOU (250 France), Nicolas DUPIN (250 France) and David ŠMAJS (203 Czech Republic, guarantor, belonging to the institution)
Edition
PLoS neglected tropical diseases, San Francisco, Public Library of Science, 2017, 1935-2735
The TEN 11q/j isolate was characterized using nested PCR followed by Sanger sequencing and/or direct Illumina sequencing. Altogether, 44 chromosomal regions were analyzed. Overall, the 11q/j isolate clustered with TEN strains Bosnia A and Iraq B as expected from previous TEN classification of the 11q/j isolate. However, the 11q/j sequence in a 505 bp-long region at the TP0488 locus was similar to Treponema pallidum subsp. pallidum (TPA) strains, but not to TEN Bosnia A and Iraq B sequences, suggesting a recombination event at this locus. Similarly, the 11q/j sequence in a 613 bp-long region at the TP0548 locus was similar to Treponema pallidum subsp. pertenue (TPE) strains, but not to TEN sequences.
Links
GAP302/12/0574, research and development project
Name: Celogenomové sekvencování v analýze genomů a transkriptomů patogenních bakterií rodu Treponema
Investor: Czech Science Foundation
GA17-25455S, research and development project
Name: Studium genomů patogenních treponem na základě analýzy jednotlivých buněk
Investor: Czech Science Foundation
GP14-29596P, research and development project
Name: Genomová variabilita treponem během experimentální infekce
Investor: Czech Science Foundation
ROZV/20/LF/2015, interní kód MU
Name: LF - Příspěvek IP 2015
Investor: Ministry of Education, Youth and Sports of the CR
ROZV/24/LF/2016, interní kód MU
Name: LF - Příspěvek IP 2016
Investor: Ministry of Education, Youth and Sports of the CR