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@inbook{1376226, author = {Kazda, Anita and Akimcheva, Svetlana and Watson, Matthew J. and Říha, Karel}, address = {New York}, booktitle = {Plant Cell Division - Methods and Protocols}, doi = {http://dx.doi.org/10.1007/978-1-4939-3142-2_13}, editor = {Marie-Cécile Caillaud}, keywords = {5-Ethynyl-2´deoxyuridine; Cell division; Cell proliferation; DNA labeling; DNA replication; EdU; Histology}, howpublished = {tištěná verze "print"}, language = {eng}, location = {New York}, isbn = {978-1-4939-3141-5}, pages = {169-182}, publisher = {Springer}, title = {Cell Proliferation Analysis Using EdU Labeling in Whole Plant and Histological Samples of Arabidopsis}, url = {http://link.springer.com/protocol/10.1007%2F978-1-4939-3142-2_13}, year = {2016} }
TY - CHAP ID - 1376226 AU - Kazda, Anita - Akimcheva, Svetlana - Watson, Matthew J. - Říha, Karel PY - 2016 TI - Cell Proliferation Analysis Using EdU Labeling in Whole Plant and Histological Samples of Arabidopsis VL - Methods in Molecular Biology, 1370 PB - Springer CY - New York SN - 9781493931415 KW - 5-Ethynyl-2´deoxyuridine KW - Cell division KW - Cell proliferation KW - DNA labeling KW - DNA replication KW - EdU KW - Histology UR - http://link.springer.com/protocol/10.1007%2F978-1-4939-3142-2_13 L2 - http://link.springer.com/protocol/10.1007%2F978-1-4939-3142-2_13 N2 - The ability to analyze cell division in both spatial and temporal dimensions within an organism is a key requirement in developmental biology. Specialized cell types within individual organs, such as those within shoot and root apical meristems, have often been identified by differences in their rates of proliferation prior to the characterization of distinguishing molecular markers. Replication-dependent labeling of DNA is a widely used method for assaying cell proliferation. The earliest approaches used radioactive labeling with tritiated thymidine, which were later followed by immunodetection of bromodeoxyuridine (BrdU). A major advance in DNA labeling came with the use of 5-ethynyl-2´deoxyuridine (EdU) which has proven to have multiple advantages over BrdU. Here we describe the methodology for analyzing EdU labeling and retention in whole plants and histological sections of Arabidopsis. ER -
KAZDA, Anita, Svetlana AKIMCHEVA, Matthew J. WATSON and Karel ŘÍHA. Cell Proliferation Analysis Using EdU Labeling in Whole Plant and Histological Samples of Arabidopsis. In Marie-Cécile Caillaud. \textit{Plant Cell Division - Methods and Protocols}. New York: Springer, 2016, p.~169-182. Methods in Molecular Biology, 1370. ISBN~978-1-4939-3141-5. Available from: https://dx.doi.org/10.1007/978-1-4939-3142-2\_{}13.
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