Cell Proliferation Analysis Using EdU Labeling in Whole Plant and Histological Samples of Arabidopsis

KAZDA, Anita, Svetlana AKIMCHEVA, Matthew J. WATSON and Karel ŘÍHA. Cell Proliferation Analysis Using EdU Labeling in Whole Plant and Histological Samples of Arabidopsis. In Marie-Cécile Caillaud. Plant Cell Division - Methods and Protocols. New York: Springer, 2016, p. 169-182. Methods in Molecular Biology, 1370. ISBN 978-1-4939-3141-5. Available from: https://dx.doi.org/10.1007/978-1-4939-3142-2_13.

Basic information

• Original name: Cell Proliferation Analysis Using EdU Labeling in Whole Plant and Histological Samples of Arabidopsis
• Authors: KAZDA, Anita (40 Austria), Svetlana AKIMCHEVA (40 Austria), Matthew J. WATSON (40 Austria) and Karel ŘÍHA (203 Czech Republic, guarantor, belonging to the institution).
• Edition: New York, Plant Cell Division - Methods and Protocols, p. 169-182, 14 pp. Methods in Molecular Biology, 1370, 2016.
• Publisher: Springer

Other information

• Original language: English
• Type of outcome: Chapter(s) of a specialized book
• Field of Study: 10608 Biochemistry and molecular biology
• Country of publisher: United States of America
• Confidentiality degree: is not subject to a state or trade secret
• Publication form: printed version "print"
• WWW: URL
• RIV identification code: RIV/00216224:14740/16:00093963
• Organization unit: Central European Institute of Technology
• ISBN: 978-1-4939-3141-5
• Doi: http://dx.doi.org/10.1007/978-1-4939-3142-2_13
• UT WoS: 000369085800014
• Keywords in English: 5-Ethynyl-2´deoxyuridine; Cell division; Cell proliferation; DNA labeling; DNA replication; EdU; Histology
• Tags: rivok

Abstract

The ability to analyze cell division in both spatial and temporal dimensions within an organism is a key requirement in developmental biology. Specialized cell types within individual organs, such as those within shoot and root apical meristems, have often been identified by differences in their rates of proliferation prior to the characterization of distinguishing molecular markers. Replication-dependent labeling of DNA is a widely used method for assaying cell proliferation. The earliest approaches used radioactive labeling with tritiated thymidine, which were later followed by immunodetection of bromodeoxyuridine (BrdU). A major advance in DNA labeling came with the use of 5-ethynyl-2´deoxyuridine (EdU) which has proven to have multiple advantages over BrdU. Here we describe the methodology for analyzing EdU labeling and retention in whole plants and histological sections of Arabidopsis.