Highland cattle and Radix labiata, the hosts of Fascioloides
magna

J 2014

Highland cattle and Radix labiata, the hosts of Fascioloides magna

LEONTOVYČ, Roman, Monika KOŠŤÁKOVÁ, Veronika SIEGELOVÁ, Klára MELOUNOVÁ, Jan PANKRÁC et. al.

Basic information

Original name

Highland cattle and Radix labiata, the hosts of Fascioloides magna

Authors

LEONTOVYČ, Roman (203 Czech Republic), Monika KOŠŤÁKOVÁ (203 Czech Republic), Veronika SIEGELOVÁ (203 Czech Republic), Klára MELOUNOVÁ (203 Czech Republic), Jan PANKRÁC (203 Czech Republic), Kristýna VRBOVÁ (203 Czech Republic), Petr HORÁK (203 Czech Republic) and Martin KAŠNÝ (203 Czech Republic, guarantor, belonging to the institution)

Edition

BMC Veterinary Research, LONDON, BIOMED CENTRAL LTD, 2014, 1746-6148

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

40301 Veterinary science

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 1.777

RIV identification code

RIV/00216224:14310/14:00093976

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1186/1746-6148-10-41

UT WoS

000334701700001

Keywords in English

Fascioloides magna; Fasciola hepatica; Radix labiata; Galba truncatula; Highland cattle; Molecular determination; ITS2; Histology; Pseudocyst

Abstract

V originále

Background: Fascioloides magna is a pathogenic fluke introduced to Europe ca 140 years ago. As it is spreading over the continent, new intermediate and definitive hosts might be involved in transmission of the parasite. In Europe, several studies reported potential new intermediate snail hosts (Radix spp.) for F. magna, and also several cases of fascioloidosis of wild and domestic animals were published. However, the data based on molecular and histological analyses confirming these findings remained unreported. This study aims to refer to unique findings of F. magna in European snails and domestic animals (the first observation in the Czech Republic in the last 30 years) and demonstrate the use of molecular techniques in determination of F. magna. Results: Two snails of R. labiata naturally infected with F. magna were found; mature cercariae and daughter rediae were observed. Maturity of cercariae was checked by histological methods, however, their ability to encyst was not confirmed. Co-infection of F. magna and Fasciola hepatica in the liver of two highland cattle bulls was proved. Adult fasciolid flukes producing eggs were found in the liver pseudocysts (F. magna) and the bile ducts (F. hepatica). Identification of intermediate hosts, intramolluscan stages, adult flukes and eggs was performed by sequencing the ITS2 region. Connection of F. magna pseudocysts with the gut (via the bile ducts) was not confirmed by means of histological and coprological examinations. Conclusions: For the first time, Radix labiata was confirmed as the snail host for F. magna under natural conditions and, together with the finding of F. magna infection in cattle, we can expect further transmission of F. magna from wildlife to livestock in localities shared by these hosts.

Links

MUNI/A/1484/2014, interní kód MU

Name: Analýzy diverzity biologických systémů různých úrovní a na různých škálách terestrického a akvatického prostředí (Acronym: BIDA4)

Investor: Masaryk University, Category A

Citovat

LEONTOVYČ, Roman, Monika KOŠŤÁKOVÁ, Veronika SIEGELOVÁ, Klára MELOUNOVÁ, Jan PANKRÁC, Kristýna VRBOVÁ, Petr HORÁK and Martin KAŠNÝ. Highland cattle and Radix labiata, the hosts of Fascioloides magna. BMC Veterinary Research. LONDON: BIOMED CENTRAL LTD, 2014, vol. 10, February, p. "nestrankovano", 7 pp. ISSN 1746-6148. Available from: https://dx.doi.org/10.1186/1746-6148-10-41.

@article{1376316,
   author = {Leontovyč, Roman and Košťáková, Monika and Siegelová, Veronika and Melounová, Klára and Pankrác, Jan and Vrbová, Kristýna and Horák, Petr and Kašný, Martin},
   article_location = {LONDON},
   article_number = {February},
   doi = {http://dx.doi.org/10.1186/1746-6148-10-41},
   keywords = {Fascioloides magna; Fasciola hepatica; Radix labiata; Galba truncatula; Highland cattle; Molecular determination; ITS2; Histology; Pseudocyst},
   language = {eng},
   issn = {1746-6148},
   journal = {BMC Veterinary Research},
   title = {Highland cattle and Radix labiata, the hosts of Fascioloides magna},
   volume = {10},
   year = {2014}
}

TY  - JOUR
ID  - 1376316
AU  - Leontovyč, Roman - Košťáková, Monika - Siegelová, Veronika - Melounová, Klára - Pankrác, Jan - Vrbová, Kristýna - Horák, Petr - Kašný, Martin
PY  - 2014
TI  - Highland cattle and Radix labiata, the hosts of Fascioloides magna
JF  - BMC Veterinary Research
VL  - 10
IS  - February
SP  - "nestrankovano"
EP  - "nestrankovano"
PB  - BIOMED CENTRAL LTD
SN  - 17466148
KW  - Fascioloides magna
KW  - Fasciola hepatica
KW  - Radix labiata
KW  - Galba truncatula
KW  - Highland cattle
KW  - Molecular determination
KW  - ITS2
KW  - Histology
KW  - Pseudocyst
N2  - Background: Fascioloides magna is a pathogenic fluke introduced to Europe ca 140 years ago. As it is spreading over the continent, new intermediate and definitive hosts might be involved in transmission of the parasite. In Europe, several studies reported potential new intermediate snail hosts (Radix spp.) for F. magna, and also several cases of fascioloidosis of wild and domestic animals were published. However, the data based on molecular and histological analyses confirming these findings remained unreported. This study aims to refer to unique findings of F. magna in European snails and domestic animals (the first observation in the Czech Republic in the last 30 years) and demonstrate the use of molecular techniques in determination of F. magna. Results: Two snails of R. labiata naturally infected with F. magna were found; mature cercariae and daughter rediae were observed. Maturity of cercariae was checked by histological methods, however, their ability to encyst was not confirmed. Co-infection of F. magna and Fasciola hepatica in the liver of two highland cattle bulls was proved. Adult fasciolid flukes producing eggs were found in the liver pseudocysts (F. magna) and the bile ducts (F. hepatica). Identification of intermediate hosts, intramolluscan stages, adult flukes and eggs was performed by sequencing the ITS2 region. Connection of F. magna pseudocysts with the gut (via the bile ducts) was not confirmed by means of histological and coprological examinations. Conclusions: For the first time, Radix labiata was confirmed as the snail host for F. magna under natural conditions and, together with the finding of F. magna infection in cattle, we can expect further transmission of F. magna from wildlife to livestock in localities shared by these hosts.
ER  -

LEONTOVYČ, Roman, Monika KOŠŤÁKOVÁ, Veronika SIEGELOVÁ, Klára MELOUNOVÁ, Jan PANKRÁC, Kristýna VRBOVÁ, Petr HORÁK and Martin KAŠNÝ. Highland cattle and Radix labiata, the hosts of Fascioloides magna. \textit{BMC Veterinary Research}. LONDON: BIOMED CENTRAL LTD, 2014, vol.~10, February, p.~''nestrankovano'', 7 pp. ISSN~1746-6148. Available from: https://dx.doi.org/10.1186/1746-6148-10-41.

Detailed Information on Publication Record