Characterization of total retinoid-like activity of compounds
produced by three common phytoplankton species

J 2016

Characterization of total retinoid-like activity of compounds produced by three common phytoplankton species

SYCHROVÁ, Eliška, Jana PRIEBOJOVÁ, Marie SMUTNÁ, Kateřina NOVÁKOVÁ, Jiří KOHOUTEK et. al.

Basic information

Original name

Characterization of total retinoid-like activity of compounds produced by three common phytoplankton species

Authors

SYCHROVÁ, Eliška (203 Czech Republic, belonging to the institution), Jana PRIEBOJOVÁ (703 Slovakia, belonging to the institution), Marie SMUTNÁ (203 Czech Republic, belonging to the institution), Kateřina NOVÁKOVÁ (203 Czech Republic, belonging to the institution), Jiří KOHOUTEK (203 Czech Republic, belonging to the institution) and Klára HILSCHEROVÁ (203 Czech Republic, guarantor, belonging to the institution)

Edition

Harmful Algae, AMSTERDAM (NETHERLANDS), Elsevier Science, 2016, 1568-9883

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10000 1. Natural Sciences

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 3.087

RIV identification code

RIV/00216224:14310/16:00088800

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1016/j.hal.2016.11.002

UT WoS

000390516200015

Keywords in English

Retinoic acid receptor; Cyanobacteria; Biomass extracts; Exudates; All-trans-retinoic acid; 9-Cis retinoic acid; Retinoid-like activity; Microcystin; Light conditions

Abstract

V originále

Phytoplankton can produce various bioactive metabolites, which may affect other organisms in the aquatic environment. This study provides the first information on the total retinoid-like activity associated with both intracellular and extracellular metabolites produced by selected phytoplankton species that could play a role in teratogenic effects and developmental disruption in exposed organisms. The studied species included a coccoid cyanobacteria, a filamentous cyanobacteria and a green alga, all of which commonly occur in freshwater bodies in Europe. Methanolic extracts from cellular material and extracellular exudates were prepared from cultures cultivated in two light-intensity variants with five replicates for each species. The retinoid-like activity was evaluated by in vitro assays along with chemical analyses of two potent retinoic acids (all-trans retinoic acid (ATRA) and 9cis-RA). The mean total retinoid-like activity of metabolites produced by the three studied species representing different phytoplankton taxonomic groups ranged from 705 to 5572 ng ATRA equivalent/g dry matter corresponding to 0.064-0.234 ng ATRA/10(6) cells. Retinoid-like activity was found in the cellular extracts of all species, while only the extracellular exudates of cyanobacteria exhibited detectable activity (41-1081 ng ATRA/L). The greatest extracellular as well as total (extra- and intra- cellular together) retinoid-like activity was detected for Microcystis aeruginosa. The two potent retinoic acids studied were more frequently detected in cellular extracts than in extracellular exudates of all species. The results indicate possible influence of light intensity and cell density on the production of metabolites with retinoid-like activity and the cyanotoxin microcystin by the studied species.

Links

GP14-29370P, research and development project

Name: Běžné druhy sinic jako producenti teratogenních retinoidních látek

Investor: Czech Science Foundation

LO1214, research and development project

Name: Centrum pro výzkum toxických látek v prostředí (Acronym: RECETOX)

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

SYCHROVÁ, Eliška, Jana PRIEBOJOVÁ, Marie SMUTNÁ, Kateřina NOVÁKOVÁ, Jiří KOHOUTEK and Klára HILSCHEROVÁ. Characterization of total retinoid-like activity of compounds produced by three common phytoplankton species. Harmful Algae. AMSTERDAM (NETHERLANDS): Elsevier Science, 2016, vol. 60, December, p. 157-166. ISSN 1568-9883. Available from: https://dx.doi.org/10.1016/j.hal.2016.11.002.

@article{1376647,
   author = {Sychrová, Eliška and Priebojová, Jana and Smutná, Marie and Nováková, Kateřina and Kohoutek, Jiří and Hilscherová, Klára},
   article_location = {AMSTERDAM (NETHERLANDS)},
   article_number = {December},
   doi = {http://dx.doi.org/10.1016/j.hal.2016.11.002},
   keywords = {Retinoic acid receptor; Cyanobacteria; Biomass extracts; Exudates; All-trans-retinoic acid; 9-Cis retinoic acid; Retinoid-like activity; Microcystin; Light conditions},
   language = {eng},
   issn = {1568-9883},
   journal = {Harmful Algae},
   title = {Characterization of total retinoid-like activity of compounds produced by three common phytoplankton species},
   url = {http://www.sciencedirect.com/science/article/pii/S156898831630021X},
   volume = {60},
   year = {2016}
}

TY  - JOUR
ID  - 1376647
AU  - Sychrová, Eliška - Priebojová, Jana - Smutná, Marie - Nováková, Kateřina - Kohoutek, Jiří - Hilscherová, Klára
PY  - 2016
TI  - Characterization of total retinoid-like activity of compounds produced by three common phytoplankton species
JF  - Harmful Algae
VL  - 60
IS  - December
SP  - 157-166
EP  - 157-166
PB  - Elsevier Science
SN  - 15689883
KW  - Retinoic acid receptor
KW  - Cyanobacteria
KW  - Biomass extracts
KW  - Exudates
KW  - All-trans-retinoic acid
KW  - 9-Cis retinoic acid
KW  - Retinoid-like activity
KW  - Microcystin
KW  - Light conditions
UR  - http://www.sciencedirect.com/science/article/pii/S156898831630021X
L2  - http://www.sciencedirect.com/science/article/pii/S156898831630021X
N2  - Phytoplankton can produce various bioactive metabolites, which may affect other organisms in the aquatic environment. This study provides the first information on the total retinoid-like activity associated with both intracellular and extracellular metabolites produced by selected phytoplankton species that could play a role in teratogenic effects and developmental disruption in exposed organisms. The studied species included a coccoid cyanobacteria, a filamentous cyanobacteria and a green alga, all of which commonly occur in freshwater bodies in Europe. Methanolic extracts from cellular material and extracellular exudates were prepared from cultures cultivated in two light-intensity variants with five replicates for each species. The retinoid-like activity was evaluated by in vitro assays along with chemical analyses of two potent retinoic acids (all-trans retinoic acid (ATRA) and 9cis-RA). The mean total retinoid-like activity of metabolites produced by the three studied species representing different phytoplankton taxonomic groups ranged from 705 to 5572 ng ATRA equivalent/g dry matter corresponding to 0.064-0.234 ng ATRA/10(6) cells. Retinoid-like activity was found in the cellular extracts of all species, while only the extracellular exudates of cyanobacteria exhibited detectable activity (41-1081 ng ATRA/L). The greatest extracellular as well as total (extra- and intra- cellular together) retinoid-like activity was detected for Microcystis aeruginosa. The two potent retinoic acids studied were more frequently detected in cellular extracts than in extracellular exudates of all species. The results indicate possible influence of light intensity and cell density on the production of metabolites with retinoid-like activity and the cyanotoxin microcystin by the studied species.
ER  -

SYCHROVÁ, Eliška, Jana PRIEBOJOVÁ, Marie SMUTNÁ, Kateřina NOVÁKOVÁ, Jiří KOHOUTEK and Klára HILSCHEROVÁ. Characterization of total retinoid-like activity of compounds produced by three common phytoplankton species. \textit{Harmful Algae}. AMSTERDAM (NETHERLANDS): Elsevier Science, 2016, vol.~60, December, p.~157-166. ISSN~1568-9883. Available from: https://dx.doi.org/10.1016/j.hal.2016.11.002.

Detailed Information on Publication Record