Problematic barcoding in flatworms: A case-study on monogeneans
and rhabdocoels (Platyhelminthes)

J 2013

Problematic barcoding in flatworms: A case-study on monogeneans and rhabdocoels (Platyhelminthes)

VANHOVE, Maarten Pieterjan, Bart TESSENS, Charlotte SCHOELINCK, Ulf JONDELIUS, D. Tim J. LITTLEWOOD et. al.

Basic information

Original name

Problematic barcoding in flatworms: A case-study on monogeneans and rhabdocoels (Platyhelminthes)

Authors

VANHOVE, Maarten Pieterjan (56 Belgium, belonging to the institution), Bart TESSENS (56 Belgium), Charlotte SCHOELINCK (124 Canada), Ulf JONDELIUS (752 Sweden), D. Tim J. LITTLEWOOD (826 United Kingdom of Great Britain and Northern Ireland), Tom ARTOIS (56 Belgium) and Tine HUYSE (56 Belgium)

Edition

ZOOKEYS, 2013, 1313-2989

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Bulgaria

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 0.917

RIV identification code

RIV/00216224:14310/13:00094005

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.3897/zookeys.365.5776

UT WoS

000329206600021

Keywords in English

mitochondrial DNA; Monogenea; primer design; ribosomal DNA; Rhabdocoela; turbellarians

Abstract

V originále

Some taxonomic groups are less amenable to mitochondrial DNA barcoding than others. Due to the paucity of molecular information of understudied groups and the huge molecular diversity within flatworms, primer design has been hampered. Indeed, all attempts to develop universal flatworm-specific COI markers have failed so far. We demonstrate how high molecular variability and contamination problems limit the possibilities for barcoding using standard COI-based protocols in flatworms. As a consequence, molecular identification methods often rely on other widely applicable markers. In the case of Monogenea, a very diverse group of platyhelminth parasites, and Rhabdocoela, representing one-fourth of all free-living flatworm taxa, this has led to a relatively high availability of nuclear ITS and 18S/28S rDNA sequences on GenBank. In a comparison of the effectiveness in species assignment we conclude that mitochondrial and nuclear ribosomal markers perform equally well. In case intraspecific information is needed, rDNA sequences can guide the selection of the appropriate (i.e. taxon-specific) COI primers if available.

Links

GBP505/12/G112, research and development project

Name: ECIP - Evropské centrum ichtyoparazitologie

Investor: Czech Science Foundation

Citovat

VANHOVE, Maarten Pieterjan, Bart TESSENS, Charlotte SCHOELINCK, Ulf JONDELIUS, D. Tim J. LITTLEWOOD, Tom ARTOIS and Tine HUYSE. Problematic barcoding in flatworms: A case-study on monogeneans and rhabdocoels (Platyhelminthes). ZOOKEYS. 2013, vol. 2013, No 365, p. 355-379. ISSN 1313-2989. Available from: https://dx.doi.org/10.3897/zookeys.365.5776.

@article{1376685,
   author = {Vanhove, Maarten Pieterjan and Tessens, Bart and Schoelinck, Charlotte and Jondelius, Ulf and Littlewood, D. Tim J. and Artois, Tom and Huyse, Tine},
   article_number = {365},
   doi = {http://dx.doi.org/10.3897/zookeys.365.5776},
   keywords = {mitochondrial DNA; Monogenea; primer design; ribosomal DNA; Rhabdocoela; turbellarians},
   language = {eng},
   issn = {1313-2989},
   journal = {ZOOKEYS},
   title = {Problematic barcoding in flatworms: A case-study on monogeneans and rhabdocoels (Platyhelminthes)},
   volume = {2013},
   year = {2013}
}

TY  - JOUR
ID  - 1376685
AU  - Vanhove, Maarten Pieterjan - Tessens, Bart - Schoelinck, Charlotte - Jondelius, Ulf - Littlewood, D. Tim J. - Artois, Tom - Huyse, Tine
PY  - 2013
TI  - Problematic barcoding in flatworms: A case-study on monogeneans and rhabdocoels (Platyhelminthes)
JF  - ZOOKEYS
VL  - 2013
IS  - 365
SP  - 355-379
EP  - 355-379
SN  - 13132989
KW  - mitochondrial DNA
KW  - Monogenea
KW  - primer design
KW  - ribosomal DNA
KW  - Rhabdocoela
KW  - turbellarians
N2  - Some taxonomic groups are less amenable to mitochondrial DNA barcoding than others. Due to the paucity of molecular information of understudied groups and the huge molecular diversity within flatworms, primer design has been hampered. Indeed, all attempts to develop universal flatworm-specific COI markers have failed so far. We demonstrate how high molecular variability and contamination problems limit the possibilities for barcoding using standard COI-based protocols in flatworms. As a consequence, molecular identification methods often rely on other widely applicable markers. In the case of Monogenea, a very diverse group of platyhelminth parasites, and Rhabdocoela, representing one-fourth of all free-living flatworm taxa, this has led to a relatively high availability of nuclear ITS and 18S/28S rDNA sequences on GenBank. In a comparison of the effectiveness in species assignment we conclude that mitochondrial and nuclear ribosomal markers perform equally well. In case intraspecific information is needed, rDNA sequences can guide the selection of the appropriate (i.e. taxon-specific) COI primers if available.
ER  -

VANHOVE, Maarten Pieterjan, Bart TESSENS, Charlotte SCHOELINCK, Ulf JONDELIUS, D. Tim J. LITTLEWOOD, Tom ARTOIS and Tine HUYSE. Problematic barcoding in flatworms: A case-study on monogeneans and rhabdocoels (Platyhelminthes). \textit{ZOOKEYS}. 2013, vol.~2013, No~365, p.~355-379. ISSN~1313-2989. Available from: https://dx.doi.org/10.3897/zookeys.365.5776.

Detailed Information on Publication Record