'Who's who' in renal sphaerosporids (Bivalvulida: Myxozoa) from
common carp, Prussian carp and goldfish - molecular
identification of cryptic species, blood stages and new members
of Sphaerospora sensu stricto

J 2013

'Who's who' in renal sphaerosporids (Bivalvulida: Myxozoa) from common carp, Prussian carp and goldfish - molecular identification of cryptic species, blood stages and new members of Sphaerospora sensu stricto

HOLZER, Astrid Sibylle, P. BARTOSOVA, H. PECKOVA, T. TYML, S. ATKINSON et. al.

Basic information

Original name

'Who's who' in renal sphaerosporids (Bivalvulida: Myxozoa) from common carp, Prussian carp and goldfish - molecular identification of cryptic species, blood stages and new members of Sphaerospora sensu stricto

Authors

HOLZER, Astrid Sibylle (203 Czech Republic), P. BARTOSOVA (203 Czech Republic), H. PECKOVA (203 Czech Republic), T. TYML (203 Czech Republic), S. ATKINSON (840 United States of America), J. BARTHOLOMEW (840 United States of America), D. SIPOS (348 Hungary), E. ESZTERBAUER (348 Hungary) and Iva DYKOVÁ (203 Czech Republic, guarantor, belonging to the institution)

Edition

Parasitology, Cambridge University Press, 2013, 0031-1820

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 2.350

RIV identification code

RIV/00216224:14310/13:00094014

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1017/S0031182012001175

UT WoS

000313256200005

Keywords in English

Sphaerospora; Myxozoa; cyprinid; morphometry; cryptic speciation; ribosomal DNA; molecular identification; blood stages; multi-species infection

Abstract

V originále

Myxozoans are a group of diverse, spore-forming metazoan microparasites bound to aquatic environments. Sphaerospora dykovae (previously S. renicola) causes renal sphaerosporosis and acute swim bladder inflammation (SBI) in juvenile Cyprinus carpio carpio, in central Europe. A morphologically similar species with comparably low pathogenicity, S. angulata has been described from C. c. carpio, Carassius auratus auratus and Carassius gibelio. To clarify uncertainties and ambiguities in taxon identification in these hosts we decided to re-investigate differences in spore morphology using a statistical approach, in combination with SSU and LSU rDNA sequence analyses. We found that developing spores of S. angulata and S. dykovae cannot be distinguished morphologically and designed a duplex PCR assay for the cryptic species that demonstrated S. dykovae is specific to C. c. carpio, whereas S. angulata infects C. a. auratus and C. gibelio. The molecular identification of myxozoan blood stages in common carp and goldfish, which had previously been ascribed to Sphaerospora spp. showed that approximately 75% of blood stages were from non-sphaerosporid coelozoic species infecting these cyprinids and more than 10% were from an alien species, Myxobilatus gasterostei, developing in sticklebacks. We hereby report non-selective myxozoan host invasion and multi-species infections, whose role in SBI still requires clarification.

Citovat

HOLZER, Astrid Sibylle, P. BARTOSOVA, H. PECKOVA, T. TYML, S. ATKINSON, J. BARTHOLOMEW, D. SIPOS, E. ESZTERBAUER and Iva DYKOVÁ. 'Who's who' in renal sphaerosporids (Bivalvulida: Myxozoa) from common carp, Prussian carp and goldfish - molecular identification of cryptic species, blood stages and new members of Sphaerospora sensu stricto. Parasitology. Cambridge University Press, 2013, vol. 140, No 1, p. 46-60. ISSN 0031-1820. Available from: https://dx.doi.org/10.1017/S0031182012001175.

@article{1376695,
   author = {Holzer, Astrid Sibylle and Bartosova, P. and Peckova, H. and Tyml, T. and Atkinson, S. and Bartholomew, J. and Sipos, D. and Eszterbauer, E. and Dyková, Iva},
   article_number = {1},
   doi = {http://dx.doi.org/10.1017/S0031182012001175},
   keywords = {Sphaerospora; Myxozoa; cyprinid; morphometry; cryptic speciation; ribosomal DNA; molecular identification; blood stages; multi-species infection},
   language = {eng},
   issn = {0031-1820},
   journal = {Parasitology},
   title = {'Who's who' in renal sphaerosporids (Bivalvulida: Myxozoa) from common carp, Prussian carp and goldfish - molecular identification of cryptic species, blood stages and new members of Sphaerospora sensu stricto},
   volume = {140},
   year = {2013}
}

TY  - JOUR
ID  - 1376695
AU  - Holzer, Astrid Sibylle - Bartosova, P. - Peckova, H. - Tyml, T. - Atkinson, S. - Bartholomew, J. - Sipos, D. - Eszterbauer, E. - Dyková, Iva
PY  - 2013
TI  - 'Who's who' in renal sphaerosporids (Bivalvulida: Myxozoa) from common carp, Prussian carp and goldfish - molecular identification of cryptic species, blood stages and new members of Sphaerospora sensu stricto
JF  - Parasitology
VL  - 140
IS  - 1
SP  - 46-60
EP  - 46-60
PB  - Cambridge University Press
SN  - 00311820
KW  - Sphaerospora
KW  - Myxozoa
KW  - cyprinid
KW  - morphometry
KW  - cryptic speciation
KW  - ribosomal DNA
KW  - molecular identification
KW  - blood stages
KW  - multi-species infection
N2  - Myxozoans are a group of diverse, spore-forming metazoan microparasites bound to aquatic environments. Sphaerospora dykovae (previously S. renicola) causes renal sphaerosporosis and acute swim bladder inflammation (SBI) in juvenile Cyprinus carpio carpio, in central Europe. A morphologically similar species with comparably low pathogenicity, S. angulata has been described from C. c. carpio, Carassius auratus auratus and Carassius gibelio. To clarify uncertainties and ambiguities in taxon identification in these hosts we decided to re-investigate differences in spore morphology using a statistical approach, in combination with SSU and LSU rDNA sequence analyses. We found that developing spores of S. angulata and S. dykovae cannot be distinguished morphologically and designed a duplex PCR assay for the cryptic species that demonstrated S. dykovae is specific to C. c. carpio, whereas S. angulata infects C. a. auratus and C. gibelio. The molecular identification of myxozoan blood stages in common carp and goldfish, which had previously been ascribed to Sphaerospora spp. showed that approximately 75% of blood stages were from non-sphaerosporid coelozoic species infecting these cyprinids and more than 10% were from an alien species, Myxobilatus gasterostei, developing in sticklebacks. We hereby report non-selective myxozoan host invasion and multi-species infections, whose role in SBI still requires clarification.
ER  -

HOLZER, Astrid Sibylle, P. BARTOSOVA, H. PECKOVA, T. TYML, S. ATKINSON, J. BARTHOLOMEW, D. SIPOS, E. ESZTERBAUER and Iva DYKOVÁ. 'Who's who' in renal sphaerosporids (Bivalvulida: Myxozoa) from common carp, Prussian carp and goldfish - molecular identification of cryptic species, blood stages and new members of Sphaerospora sensu stricto. \textit{Parasitology}. Cambridge University Press, 2013, vol.~140, No~1, p.~46-60. ISSN~0031-1820. Available from: https://dx.doi.org/10.1017/S0031182012001175.

Detailed Information on Publication Record