Novel Temperate Phages of Salmonella enterica subsp salamae and
subsp diarizonae and Their Activity against Pathogenic
S-enterica subsp enterica Isolates

J 2017

Novel Temperate Phages of Salmonella enterica subsp salamae and subsp diarizonae and Their Activity against Pathogenic S-enterica subsp enterica Isolates

MIKALOVÁ, Lenka, Juraj BOSÁK, Hana HŘÍBKOVÁ, Daniela DĚDIČOVÁ, Oldřich BENADA et. al.

Basic information

Original name

Novel Temperate Phages of Salmonella enterica subsp salamae and subsp diarizonae and Their Activity against Pathogenic S-enterica subsp enterica Isolates

Authors

MIKALOVÁ, Lenka (203 Czech Republic, belonging to the institution), Juraj BOSÁK (703 Slovakia, belonging to the institution), Hana HŘÍBKOVÁ (203 Czech Republic, belonging to the institution), Daniela DĚDIČOVÁ (203 Czech Republic), Oldřich BENADA (203 Czech Republic), Jan ŠMARDA (203 Czech Republic, belonging to the institution) and David ŠMAJS (203 Czech Republic, guarantor, belonging to the institution)

Edition

Plos one, San Francisco, Public Library of Science, 2017, 1932-6203

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10606 Microbiology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 2.766

RIV identification code

RIV/00216224:14110/17:00094691

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1371/journal.pone.0170734

UT WoS

000396160500007

Keywords in English

TAILED-BACTERIOPHAGES; COMPARATIVE GENOMICS; ENORMOUS DIVERSITY; ESCHERICHIA-COLI; EVOLUTION; TAXONOMY; PROPHAGES; BACTERIAL; ORIGINS; SERVER

Abstract

V originále

Forty strains of Salmonella enterica (S. enterica) subspecies salamae (II), arizonae (IIIa), diarizonae (IIIb), and houtenae (IV) were isolated from human or environmental samples and tested for bacteriophage production. Production of bacteriophages was observed in 15 S. enterica strains (37.5%) belonging to either the subspecies salamae (8 strains) or diarizonae (7 strains). Activity of phages was tested against 52 pathogenic S. enterica subsp. enterica isolates and showed that phages produced by subsp. salamae had broader activity against pathogenic salmonellae compared to phages from the subsp. diarizonae. All 15 phages were analyzed using PCR amplification of phage-specific regions and 9 different amplification profiles were identified. Five phages (SEN1, SEN4, SEN5, SEN22, and SEN34) were completely sequenced and classified as temperate phages. Phages SEN4 and SEN5 were genetically identical, thus representing a single phage type (i.e. SEN4/5). SEN1 and SEN4/5 fit into the group of P2-like phages, while the SEN22 phage showed sequence relatedness to P22-like phages. Interestingly, while phage SEN34 was genetically distantly related to Lambda-like phages (Siphoviridae), it had the morphology of the Myoviridae family. Based on sequence analysis and electron microscopy, phages SEN1 and SEN4/5 were members of the Myoviridae family and phage SEN22 belonged to the Podoviridae family.

Links

GA16-21649S, research and development project

Name: Molekulární charakterizace nových bakteriocinů identifikovaných v rodech Escherichia a Shigella

Investor: Czech Science Foundation

MUNI/11/InGA04/2014, interní kód MU

Name: Alternativní antimikrobiální látky: studium vazby kolicinů na povrch patogenní bakterie

Investor: Masaryk University

ROZV/20/LF/2015, interní kód MU

Name: LF - Příspěvek IP 2015

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

MIKALOVÁ, Lenka, Juraj BOSÁK, Hana HŘÍBKOVÁ, Daniela DĚDIČOVÁ, Oldřich BENADA, Jan ŠMARDA and David ŠMAJS. Novel Temperate Phages of Salmonella enterica subsp salamae and subsp diarizonae and Their Activity against Pathogenic S-enterica subsp enterica Isolates. Plos one. San Francisco: Public Library of Science, 2017, vol. 12, No 1, p. 1-14. ISSN 1932-6203. Available from: https://dx.doi.org/10.1371/journal.pone.0170734.

@article{1376729,
   author = {Mikalová, Lenka and Bosák, Juraj and Hříbková, Hana and Dědičová, Daniela and Benada, Oldřich and Šmarda, Jan and Šmajs, David},
   article_location = {San Francisco},
   article_number = {1},
   doi = {http://dx.doi.org/10.1371/journal.pone.0170734},
   keywords = {TAILED-BACTERIOPHAGES; COMPARATIVE GENOMICS; ENORMOUS DIVERSITY; ESCHERICHIA-COLI; EVOLUTION; TAXONOMY; PROPHAGES; BACTERIAL; ORIGINS; SERVER},
   language = {eng},
   issn = {1932-6203},
   journal = {Plos one},
   title = {Novel Temperate Phages of Salmonella enterica subsp salamae and subsp diarizonae and Their Activity against Pathogenic S-enterica subsp enterica Isolates},
   volume = {12},
   year = {2017}
}

TY  - JOUR
ID  - 1376729
AU  - Mikalová, Lenka - Bosák, Juraj - Hříbková, Hana - Dědičová, Daniela - Benada, Oldřich - Šmarda, Jan - Šmajs, David
PY  - 2017
TI  - Novel Temperate Phages of Salmonella enterica subsp salamae and subsp diarizonae and Their Activity against Pathogenic S-enterica subsp enterica Isolates
JF  - Plos one
VL  - 12
IS  - 1
SP  - 1-14
EP  - 1-14
PB  - Public Library of Science
SN  - 19326203
KW  - TAILED-BACTERIOPHAGES
KW  - COMPARATIVE GENOMICS
KW  - ENORMOUS DIVERSITY
KW  - ESCHERICHIA-COLI
KW  - EVOLUTION
KW  - TAXONOMY
KW  - PROPHAGES
KW  - BACTERIAL
KW  - ORIGINS
KW  - SERVER
N2  - Forty strains of Salmonella enterica (S. enterica) subspecies salamae (II), arizonae (IIIa), diarizonae (IIIb), and houtenae (IV) were isolated from human or environmental samples and tested for bacteriophage production. Production of bacteriophages was observed in 15 S. enterica strains (37.5%) belonging to either the subspecies salamae (8 strains) or diarizonae (7 strains). Activity of phages was tested against 52 pathogenic S. enterica subsp. enterica isolates and showed that phages produced by subsp. salamae had broader activity against pathogenic salmonellae compared to phages from the subsp. diarizonae. All 15 phages were analyzed using PCR amplification of phage-specific regions and 9 different amplification profiles were identified. Five phages (SEN1, SEN4, SEN5, SEN22, and SEN34) were completely sequenced and classified as temperate phages. Phages SEN4 and SEN5 were genetically identical, thus representing a single phage type (i.e. SEN4/5). SEN1 and SEN4/5 fit into the group of P2-like phages, while the SEN22 phage showed sequence relatedness to P22-like phages. Interestingly, while phage SEN34 was genetically distantly related to Lambda-like phages (Siphoviridae), it had the morphology of the Myoviridae family. Based on sequence analysis and electron microscopy, phages SEN1 and SEN4/5 were members of the Myoviridae family and phage SEN22 belonged to the Podoviridae family.
ER  -

MIKALOVÁ, Lenka, Juraj BOSÁK, Hana HŘÍBKOVÁ, Daniela DĚDIČOVÁ, Oldřich BENADA, Jan ŠMARDA and David ŠMAJS. Novel Temperate Phages of Salmonella enterica subsp salamae and subsp diarizonae and Their Activity against Pathogenic S-enterica subsp enterica Isolates. \textit{Plos one}. San Francisco: Public Library of Science, 2017, vol.~12, No~1, p.~1-14. ISSN~1932-6203. Available from: https://dx.doi.org/10.1371/journal.pone.0170734.

Detailed Information on Publication Record