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@article{1376729, author = {Mikalová, Lenka and Bosák, Juraj and Hříbková, Hana and Dědičová, Daniela and Benada, Oldřich and Šmarda, Jan and Šmajs, David}, article_location = {San Francisco}, article_number = {1}, doi = {http://dx.doi.org/10.1371/journal.pone.0170734}, keywords = {TAILED-BACTERIOPHAGES; COMPARATIVE GENOMICS; ENORMOUS DIVERSITY; ESCHERICHIA-COLI; EVOLUTION; TAXONOMY; PROPHAGES; BACTERIAL; ORIGINS; SERVER}, language = {eng}, issn = {1932-6203}, journal = {Plos one}, title = {Novel Temperate Phages of Salmonella enterica subsp salamae and subsp diarizonae and Their Activity against Pathogenic S-enterica subsp enterica Isolates}, volume = {12}, year = {2017} }
TY - JOUR ID - 1376729 AU - Mikalová, Lenka - Bosák, Juraj - Hříbková, Hana - Dědičová, Daniela - Benada, Oldřich - Šmarda, Jan - Šmajs, David PY - 2017 TI - Novel Temperate Phages of Salmonella enterica subsp salamae and subsp diarizonae and Their Activity against Pathogenic S-enterica subsp enterica Isolates JF - Plos one VL - 12 IS - 1 SP - 1-14 EP - 1-14 PB - Public Library of Science SN - 19326203 KW - TAILED-BACTERIOPHAGES KW - COMPARATIVE GENOMICS KW - ENORMOUS DIVERSITY KW - ESCHERICHIA-COLI KW - EVOLUTION KW - TAXONOMY KW - PROPHAGES KW - BACTERIAL KW - ORIGINS KW - SERVER N2 - Forty strains of Salmonella enterica (S. enterica) subspecies salamae (II), arizonae (IIIa), diarizonae (IIIb), and houtenae (IV) were isolated from human or environmental samples and tested for bacteriophage production. Production of bacteriophages was observed in 15 S. enterica strains (37.5%) belonging to either the subspecies salamae (8 strains) or diarizonae (7 strains). Activity of phages was tested against 52 pathogenic S. enterica subsp. enterica isolates and showed that phages produced by subsp. salamae had broader activity against pathogenic salmonellae compared to phages from the subsp. diarizonae. All 15 phages were analyzed using PCR amplification of phage-specific regions and 9 different amplification profiles were identified. Five phages (SEN1, SEN4, SEN5, SEN22, and SEN34) were completely sequenced and classified as temperate phages. Phages SEN4 and SEN5 were genetically identical, thus representing a single phage type (i.e. SEN4/5). SEN1 and SEN4/5 fit into the group of P2-like phages, while the SEN22 phage showed sequence relatedness to P22-like phages. Interestingly, while phage SEN34 was genetically distantly related to Lambda-like phages (Siphoviridae), it had the morphology of the Myoviridae family. Based on sequence analysis and electron microscopy, phages SEN1 and SEN4/5 were members of the Myoviridae family and phage SEN22 belonged to the Podoviridae family. ER -
MIKALOVÁ, Lenka, Juraj BOSÁK, Hana HŘÍBKOVÁ, Daniela DĚDIČOVÁ, Oldřich BENADA, Jan ŠMARDA and David ŠMAJS. Novel Temperate Phages of Salmonella enterica subsp salamae and subsp diarizonae and Their Activity against Pathogenic S-enterica subsp enterica Isolates. \textit{Plos one}. San Francisco: Public Library of Science, 2017, vol.~12, No~1, p.~1-14. ISSN~1932-6203. Available from: https://dx.doi.org/10.1371/journal.pone.0170734.
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