MIKALOVÁ, Lenka, Juraj BOSÁK, Hana HŘÍBKOVÁ, Daniela DĚDIČOVÁ, Oldřich BENADA, Jan ŠMARDA and David ŠMAJS. Novel Temperate Phages of Salmonella enterica subsp salamae and subsp diarizonae and Their Activity against Pathogenic S-enterica subsp enterica Isolates. Plos one. San Francisco: Public Library of Science, vol. 12, No 1, p. 1-14. ISSN 1932-6203. doi:10.1371/journal.pone.0170734. 2017.
Other formats:   BibTeX LaTeX RIS
Basic information
Original name Novel Temperate Phages of Salmonella enterica subsp salamae and subsp diarizonae and Their Activity against Pathogenic S-enterica subsp enterica Isolates
Authors MIKALOVÁ, Lenka (203 Czech Republic, belonging to the institution), Juraj BOSÁK (703 Slovakia, belonging to the institution), Hana HŘÍBKOVÁ (203 Czech Republic, belonging to the institution), Daniela DĚDIČOVÁ (203 Czech Republic), Oldřich BENADA (203 Czech Republic), Jan ŠMARDA (203 Czech Republic, belonging to the institution) and David ŠMAJS (203 Czech Republic, guarantor, belonging to the institution).
Edition Plos one, San Francisco, Public Library of Science, 2017, 1932-6203.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10606 Microbiology
Country of publisher United States of America
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 2.766
RIV identification code RIV/00216224:14110/17:00094691
Organization unit Faculty of Medicine
Doi http://dx.doi.org/10.1371/journal.pone.0170734
UT WoS 000396160500007
Keywords in English TAILED-BACTERIOPHAGES; COMPARATIVE GENOMICS; ENORMOUS DIVERSITY; ESCHERICHIA-COLI; EVOLUTION; TAXONOMY; PROPHAGES; BACTERIAL; ORIGINS; SERVER
Tags EL OK
Tags International impact, Reviewed
Changed by Changed by: Soňa Böhmová, učo 232884. Changed: 20/3/2018 13:39.
Abstract
Forty strains of Salmonella enterica (S. enterica) subspecies salamae (II), arizonae (IIIa), diarizonae (IIIb), and houtenae (IV) were isolated from human or environmental samples and tested for bacteriophage production. Production of bacteriophages was observed in 15 S. enterica strains (37.5%) belonging to either the subspecies salamae (8 strains) or diarizonae (7 strains). Activity of phages was tested against 52 pathogenic S. enterica subsp. enterica isolates and showed that phages produced by subsp. salamae had broader activity against pathogenic salmonellae compared to phages from the subsp. diarizonae. All 15 phages were analyzed using PCR amplification of phage-specific regions and 9 different amplification profiles were identified. Five phages (SEN1, SEN4, SEN5, SEN22, and SEN34) were completely sequenced and classified as temperate phages. Phages SEN4 and SEN5 were genetically identical, thus representing a single phage type (i.e. SEN4/5). SEN1 and SEN4/5 fit into the group of P2-like phages, while the SEN22 phage showed sequence relatedness to P22-like phages. Interestingly, while phage SEN34 was genetically distantly related to Lambda-like phages (Siphoviridae), it had the morphology of the Myoviridae family. Based on sequence analysis and electron microscopy, phages SEN1 and SEN4/5 were members of the Myoviridae family and phage SEN22 belonged to the Podoviridae family.
Links
GA16-21649S, research and development projectName: Molekulární charakterizace nových bakteriocinů identifikovaných v rodech Escherichia a Shigella
Investor: Czech Science Foundation
MUNI/11/InGA04/2014, interní kód MUName: Alternativní antimikrobiální látky: studium vazby kolicinů na povrch patogenní bakterie
Investor: Masaryk University
ROZV/20/LF/2015, interní kód MUName: LF - Příspěvek IP 2015
Investor: Ministry of Education, Youth and Sports of the CR
PrintDisplayed: 28/3/2024 18:22