J 2017

Molecular typing of Treponema pallidum isolates from Buenos Aires, Argentina: Frequent Nichols-like isolates and low levels of macrolide resistance

VAULET, Lucía Gallo, Linda GRILLOVÁ, Lenka MIKALOVÁ, Ricardo CASCO, Marcelo Rodríguez FERMEPIN et. al.

Basic information

Original name

Molecular typing of Treponema pallidum isolates from Buenos Aires, Argentina: Frequent Nichols-like isolates and low levels of macrolide resistance

Authors

VAULET, Lucía Gallo (32 Argentina), Linda GRILLOVÁ (203 Czech Republic, belonging to the institution), Lenka MIKALOVÁ (203 Czech Republic, belonging to the institution), Ricardo CASCO (32 Argentina), Marcelo Rodríguez FERMEPIN (32 Argentina), María A. PANDO (32 Argentina) and David ŠMAJS (203 Czech Republic, guarantor, belonging to the institution)

Edition

Plos one, San Francisco, Public Library of Science, 2017, 1932-6203

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10606 Microbiology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 2.766

RIV identification code

RIV/00216224:14110/17:00094692

Organization unit

Faculty of Medicine

DOI

UT WoS

000394688200167

Keywords in English

SYPHILIS-CAUSING STRAINS; RIBOSOMAL-RNA A2058G; AZITHROMYCIN RESISTANCE; SAN-FRANCISCO; UNITED-STATES; TP0548 GENE; PREVALENCE; CHINA; EPIDEMIOLOGY; MUTATIONS

Tags

Tags

International impact, Reviewed
Změněno: 20/3/2018 12:05, Soňa Böhmová

Abstract

V originále

A total of 54 clinical samples, including genital lesion swabs, whole blood and cerebrospinal fluid from patients diagnosed with syphilis were collected in 2006 and in 2013 in Buenos Aires, Argentina. Treponemal DNA was detected in 43 of the analyzed samples (79.6%) and further analyzed using Sequencing-based molecular typing (SBMT) and Enhanced CDC-typing (ECDCT). By SBMT, 10 different Treponema pallidum subsp. pallidum (TPA) genotypes were found, of which six were related to the TPA SS14 strain, and four to the TPA Nichols strain. The 23S rRNA gene was amplified in samples isolated from 42 patients, and in six of them (14.3%), either the A2058G (four patients, 9.5%) or the A2059G (two patients, 4.8%) mutations were found. In addition to Taiwan, Madagascar and Peru, Argentina is another country where the prevalence of Nichols-like isolates (26.8%) is greater than 10%.

Links

GAP302/12/0574, research and development project
Name: Celogenomové sekvencování v analýze genomů a transkriptomů patogenních bakterií rodu Treponema
Investor: Czech Science Foundation
GA17-25455S, research and development project
Name: Studium genomů patogenních treponem na základě analýzy jednotlivých buněk
Investor: Czech Science Foundation
ROZV/20/LF/2015, interní kód MU
Name: LF - Příspěvek IP 2015
Investor: Ministry of Education, Youth and Sports of the CR
7AMB13AR020, research and development project
Name: Molekulární typování původce syfilis v Jižní Americe (Argentinská republika) a v Evropě (Česká republika). (Acronym: MOLTYP)
Investor: Ministry of Education, Youth and Sports of the CR