Detailed Information on Publication Record
2017
Cryo-electron Microscopy Study of the Genome Release of the Dicistrovirus Israeli Acute Bee Paralysis Virus
MULLAPUDI, Edukondalu, Tibor FÜZIK, Antonin PRIDAL and Pavel PLEVKABasic information
Original name
Cryo-electron Microscopy Study of the Genome Release of the Dicistrovirus Israeli Acute Bee Paralysis Virus
Authors
MULLAPUDI, Edukondalu (356 India, belonging to the institution), Tibor FÜZIK (703 Slovakia, belonging to the institution), Antonin PRIDAL (203 Czech Republic) and Pavel PLEVKA (203 Czech Republic, guarantor, belonging to the institution)
Edition
JOURNAL OF VIROLOGY, WASHINGTON, AMER SOC MICROBIOLOGY, 2017, 0022-538X
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10600 1.6 Biological sciences
Country of publisher
United States of America
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 4.368
RIV identification code
RIV/00216224:14740/17:00096372
Organization unit
Central European Institute of Technology
UT WoS
000393883300022
Keywords in English
virus; Apis mellifera; honey bee; honeybee; Picornavirales; Dicistroviridae; Aparavirus; virion; structure; cryo; electron microscopy; capsid; genome; release; uncoating; colony collapse disorder; CCD; empty
Tags
International impact, Reviewed
Změněno: 23/2/2018 13:18, Mgr. Pavla Foltynová, Ph.D.
Abstract
V originále
Viruses of the family Dicistroviridae can cause substantial economic damage by infecting agriculturally important insects. Israeli acute bee paralysis virus (IAPV) causes honeybee colony collapse disorder in the United States. High-resolution molecular details of the genome delivery mechanism of dicistroviruses are unknown. Here we present a cryo-electron microscopy analysis of IAPV virions induced to release their genomes in vitro. We determined structures of full IAPV virions primed to release their genomes to a resolution of 3.3 angstrom and of empty capsids to a resolution of 3.9 angstrom. We show that IAPV does not form expanded A particles before genome release as in the case of related enteroviruses of the family Picornaviridae. The structural changes observed in the empty IAPV particles include detachment of the VP4 minor capsid proteins from the inner face of the capsid and partial loss of the structure of the N-terminal arms of the VP2 capsid proteins. Unlike the case for many picornaviruses, the empty particles of IAPV are not expanded relative to the native virions and do not contain pores in their capsids that might serve as channels for genome release. Therefore, rearrangement of a unique region of the capsid is probably required for IAPV genome release. IMPORTANCE Honeybee populations in Europe and North America are declining due to pressure from pathogens, including viruses. Israeli acute bee paralysis virus (IAPV), a member of the family Dicistroviridae, causes honeybee colony collapse disorder in the United States. The delivery of virus genomes into host cells is necessary for the initiation of infection. Here we present a structural cryo-electron microscopy analysis of IAPV particles induced to release their genomes. We show that genome release is not preceded by an expansion of IAPV virions as in the case of related picornaviruses that infect vertebrates. Furthermore, minor capsid proteins detach from the capsid upon genome release. The genome leaves behind empty particles that have compact protein shells.
Links
| LM2010005, research and development project |
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| LM2015043, research and development project |
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| LQ1601, research and development project |
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