J 2014

Structure of the 3.3 MDa, in vitro assembled, hubless bacteriophage T4 baseplate

YAP, Moh Lan, Thomas KLOSE, Pavel PLEVKA, Anastasia AKSYUK, Xinzheng ZHANG et. al.

Basic information

Original name

Structure of the 3.3 MDa, in vitro assembled, hubless bacteriophage T4 baseplate

Authors

YAP, Moh Lan, Thomas KLOSE, Pavel PLEVKA, Anastasia AKSYUK, Xinzheng ZHANG, Fumio ARISAKA and Michael G. ROSSMANN

Edition

Journal of Structural Biology, UNITED STATES, ACADEMIC PRESS INC ELSEVIER SCIENCE, 2014, 1047-8477

Other information

Language

English

Type of outcome

Article in a journal

Field of Study

10600 1.6 Biological sciences

Country of publisher

United States of America

Confidentiality degree

is not subject to a state or trade secret

Impact factor

Impact factor: 3.231

Organization unit

Central European Institute of Technology

DOI

UT WoS

000340217300001

Keywords in English

Bacteriophage T4; Baseplate; X-ray crystallography; Low resolution

Tags

Changed: 29/3/2017 10:58, Mgr. Eva Špillingová

Abstract

V originále

The bacteriophage T4 baseplate is the control center of the virus, where the recognition of an Escherichia coli host by the long tail fibers is translated into a signal to initiate infection. The short tail fibers unfold from the baseplate for firm attachment to the host, followed by shrinkage of the tail sheath that causes the tail tube to enter and cross the periplasmic space ending with injection of the genome into the host. During this process, the 6.5 MDa baseplate changes its structure from a "dome" shape to a "star" shape. An in vitro assembled hubless baseplate has been crystallized. It consists of six copies of the recombinantly expressed trimeric gene product (gp) 10, monomeric gp7, dimeric gp8, dimeric gp6 and monomeric gp53. The diffraction pattern extends, at most, to 4.0 angstrom resolution. The known partial structures of gp10, gp8, and gp6 and their relative position in the baseplate derived from earlier electron microscopy studies were used for molecular replacement. An electron density map has been calculated based on molecular replacement, single isomorphous replacement with anomalous dispersion data and 2-fold non-crystallographic symmetry averaging between two baseplate wedges in the crystallographic asymmetric unit. The current electron density map indicates that there are structural changes in the gp6, gp8, and gp10 oligomers compared to their structures when separately crystallized. Additional density is also visible corresponding to gp7, gp53 and the unknown parts of gp10 and gp6. (C) 2014 Elsevier Inc. All rights reserved.