Detailed Information on Publication Record
2013
Dengue structure differs at the temperatures of its human and mosquito hosts
ZHANG, Xinzheng, Ju SHENG, Pavel PLEVKA, Richard J. KUHN, Michael S. DIAMOND et. al.Basic information
Original name
Dengue structure differs at the temperatures of its human and mosquito hosts
Authors
ZHANG, Xinzheng, Ju SHENG, Pavel PLEVKA, Richard J. KUHN, Michael S. DIAMOND and Michael G. ROSSMANN
Edition
Proceedings of the National Academy of Sciences of the United States of America, WASHINGTON, National Academy of Sciences, 2013, 0027-8424
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10600 1.6 Biological sciences
Country of publisher
United States of America
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 9.809
Organization unit
Central European Institute of Technology
UT WoS
000318677300049
Keywords in English
host temperature; irreversible and conformational change; temperature dependence; cryo-EM
Tags
Změněno: 29/3/2017 12:19, Mgr. Eva Špillingová
Abstract
V originále
We report on a conformational transition of dengue virus when changing the temperature from that present in its mosquito vectors to that of its human host. Using cryoelectron microscopy, we show that although the virus has a smooth surface, a diameter of similar to 500 angstrom, and little exposed membrane at room temperature, the virions have a bumpy appearance with a diameter of similar to 550 angstrom and some exposed membrane at 37 degrees C. The bumpy structure at 37 degrees C was found to be similar to the previously predicted structure of an intermediate between the smooth mature and fusogenic forms. As humans have a body temperature of 37 degrees C, the bumpy form of the virus would be the form present in humans. Thus, optimal dengue virus vaccines should induce antibodies that preferentially recognize epitopes exposed on the bumpy form of the virus.