| PLEVKA, Pavel, Anthony J. BATTISTI, Dennis C. WINKLER, Kaspars TARS, Heather A. HOLDAWAY, Carol M. BATOR and Michael G. ROSSMANN. Sample Preparation Induced Artifacts in Cryo-Electron Tomographs. Microscopy and Microanalysis. Saarbrücken: Cambridge University Press, 2012, vol. 18, No 5, p. 1043-1048. ISSN 1431-9276. Available from: https://dx.doi.org/10.1017/S1431927612001298. |
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@article{1376810,
author = {Plevka, Pavel and Battisti, Anthony J. and Winkler, Dennis C. and Tars, Kaspars and Holdaway, Heather A. and Bator, Carol M. and Rossmann, Michael G.},
article_location = {Saarbrücken},
article_number = {5},
doi = {http://dx.doi.org/10.1017/S1431927612001298},
keywords = {cryo-EM; microscopy; ice-vacuum interface; artifacts; virus; tomography},
language = {eng},
issn = {1431-9276},
journal = {Microscopy and Microanalysis},
title = {Sample Preparation Induced Artifacts in Cryo-Electron Tomographs},
volume = {18},
year = {2012}
}
TY - JOUR
ID - 1376810
AU - Plevka, Pavel - Battisti, Anthony J. - Winkler, Dennis C. - Tars, Kaspars - Holdaway, Heather A. - Bator, Carol M. - Rossmann, Michael G.
PY - 2012
TI - Sample Preparation Induced Artifacts in Cryo-Electron Tomographs
JF - Microscopy and Microanalysis
VL - 18
IS - 5
SP - 1043-1048
EP - 1043-1048
PB - Cambridge University Press
SN - 14319276
KW - cryo-EM
KW - microscopy
KW - ice-vacuum interface
KW - artifacts
KW - virus
KW - tomography
N2 - We investigated the effects of sample preparation and of the exposure to an electron beam on particles in cryo-electron tomographs. Various virus particles with icosahedral symmetry were examined, allowing a comparison of symmetrically related components that should be identical in structure but might be affected differently by these imaging artifacts. Comparison of tomographic reconstructions with previously determined structures established by an independent method showed that neither freezing nor electron beam exposure produced a significant amount of shrinkage along the z axis (thickness). However, we observed damage to regions of the particles located close to the surface of the vitreous ice.
ER -
PLEVKA, Pavel, Anthony J. BATTISTI, Dennis C. WINKLER, Kaspars TARS, Heather A. HOLDAWAY, Carol M. BATOR and Michael G. ROSSMANN. Sample Preparation Induced Artifacts in Cryo-Electron Tomographs. \textit{Microscopy and Microanalysis}. Saarbrücken: Cambridge University Press, 2012, vol.~18, No~5, p.~1043-1048. ISSN~1431-9276. Available from: https://dx.doi.org/10.1017/S1431927612001298.
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