Structure and assembly of a paramyxovirus matrix protein

J 2012

Structure and assembly of a paramyxovirus matrix protein

BATTISTI, Anthony J., Geng MENG, Dennis C. WINKLER, Lori W. MCGINNES, Pavel PLEVKA et. al.

Basic information

Original name

Structure and assembly of a paramyxovirus matrix protein

Authors

BATTISTI, Anthony J., Geng MENG, Dennis C. WINKLER, Lori W. MCGINNES, Pavel PLEVKA, Alasdair C. STEVEN, Trudy G. MORRISON and Michael G. ROSSMANN

Edition

Proceedings of the National Academy of Sciences of the United States of America, WASHINGTON, National Academy of Sciences, 2012, 0027-8424

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 9.737

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1073/pnas.1210275109

UT WoS

000308565300031

Keywords in English

NEWCASTLE-DISEASE VIRUS; RESPIRATORY SYNCYTIAL VIRUS; CRYSTAL-STRUCTURE; SENDAI-VIRUS; PARTICLES; REVEALS; NUCLEOCAPSIDS; GLYCOPROTEINS; ECTODOMAIN; EVOLUTION

Abstract

V originále

Many pleomorphic, lipid-enveloped viruses encode matrix proteins that direct their assembly and budding, but the mechanism of this process is unclear. We have combined X-ray crystallography and cryoelectron tomography to show that the matrix protein of Newcastle disease virus, a paramyxovirus and relative of measles virus, forms dimers that assemble into pseudotetrameric arrays that generate the membrane curvature necessary for virus budding. We show that the glycoproteins are anchored in the gaps between the matrix proteins and that the helical nucleocapsids are associated in register with the matrix arrays. About 90% of virions lack matrix arrays, suggesting that, in agreement with previous biological observations, the matrix protein needs to dissociate from the viral membrane during maturation, as is required for fusion and release of the nucleocapsid into the host's cytoplasm. Structure and sequence conservation imply that other paramyxovirus matrix proteins function similarly.

Citovat

BATTISTI, Anthony J., Geng MENG, Dennis C. WINKLER, Lori W. MCGINNES, Pavel PLEVKA, Alasdair C. STEVEN, Trudy G. MORRISON and Michael G. ROSSMANN. Structure and assembly of a paramyxovirus matrix protein. Proceedings of the National Academy of Sciences of the United States of America. WASHINGTON: National Academy of Sciences, 2012, vol. 109, No 35, p. 13996-14000. ISSN 0027-8424. Available from: https://dx.doi.org/10.1073/pnas.1210275109.

@article{1376815,
   author = {Battisti, Anthony J. and Meng, Geng and Winkler, Dennis C. and McGinnes, Lori W. and Plevka, Pavel and Steven, Alasdair C. and Morrison, Trudy G. and Rossmann, Michael G.},
   article_location = {WASHINGTON},
   article_number = {35},
   doi = {http://dx.doi.org/10.1073/pnas.1210275109},
   keywords = {NEWCASTLE-DISEASE VIRUS; RESPIRATORY SYNCYTIAL VIRUS; CRYSTAL-STRUCTURE; SENDAI-VIRUS; PARTICLES; REVEALS; NUCLEOCAPSIDS; GLYCOPROTEINS; ECTODOMAIN; EVOLUTION},
   language = {eng},
   issn = {0027-8424},
   journal = {Proceedings of the National Academy of Sciences of the United States of America},
   title = {Structure and assembly of a paramyxovirus matrix protein},
   volume = {109},
   year = {2012}
}

TY  - JOUR
ID  - 1376815
AU  - Battisti, Anthony J. - Meng, Geng - Winkler, Dennis C. - McGinnes, Lori W. - Plevka, Pavel - Steven, Alasdair C. - Morrison, Trudy G. - Rossmann, Michael G.
PY  - 2012
TI  - Structure and assembly of a paramyxovirus matrix protein
JF  - Proceedings of the National Academy of Sciences of the United States of America
VL  - 109
IS  - 35
SP  - 13996-14000
EP  - 13996-14000
PB  - National Academy of Sciences
SN  - 00278424
KW  - NEWCASTLE-DISEASE VIRUS
KW  - RESPIRATORY SYNCYTIAL VIRUS
KW  - CRYSTAL-STRUCTURE
KW  - SENDAI-VIRUS
KW  - PARTICLES
KW  - REVEALS
KW  - NUCLEOCAPSIDS
KW  - GLYCOPROTEINS
KW  - ECTODOMAIN
KW  - EVOLUTION
N2  - Many pleomorphic, lipid-enveloped viruses encode matrix proteins that direct their assembly and budding, but the mechanism of this process is unclear. We have combined X-ray crystallography and cryoelectron tomography to show that the matrix protein of Newcastle disease virus, a paramyxovirus and relative of measles virus, forms dimers that assemble into pseudotetrameric arrays that generate the membrane curvature necessary for virus budding. We show that the glycoproteins are anchored in the gaps between the matrix proteins and that the helical nucleocapsids are associated in register with the matrix arrays. About 90% of virions lack matrix arrays, suggesting that, in agreement with previous biological observations, the matrix protein needs to dissociate from the viral membrane during maturation, as is required for fusion and release of the nucleocapsid into the host's cytoplasm. Structure and sequence conservation imply that other paramyxovirus matrix proteins function similarly.
ER  -

BATTISTI, Anthony J., Geng MENG, Dennis C. WINKLER, Lori W. MCGINNES, Pavel PLEVKA, Alasdair C. STEVEN, Trudy G. MORRISON and Michael G. ROSSMANN. Structure and assembly of a paramyxovirus matrix protein. \textit{Proceedings of the National Academy of Sciences of the United States of America}. WASHINGTON: National Academy of Sciences, 2012, vol.~109, No~35, p.~13996-14000. ISSN~0027-8424. Available from: https://dx.doi.org/10.1073/pnas.1210275109.

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