Maturation of flaviviruses starts from one or more
icosahedrally independent nucleation centres

J 2011

Maturation of flaviviruses starts from one or more icosahedrally independent nucleation centres

PLEVKA, Pavel, Anthony J. BATTISTI, Jiraphan JUNJHON, Dennis C. WINKLER, Heather A. HOLDAWAY et. al.

Basic information

Original name

Maturation of flaviviruses starts from one or more icosahedrally independent nucleation centres

Authors

PLEVKA, Pavel, Anthony J. BATTISTI, Jiraphan JUNJHON, Dennis C. WINKLER, Heather A. HOLDAWAY, Poonsook KEELAPANG, Nopporn SITTISOMBUT, Richard J. KUHN, Alasdair C. STEVEN and Michael G. ROSSMANN

Edition

EMBO reports, Hoboken, Wiley-Blackwell, 2011, 1469-221X

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 7.355

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1038/embor.2011.75

UT WoS

000291148200023

Keywords in English

dengue virus 2; maturation; mosaic; mutants; symmetry

Abstract

V originále

Flaviviruses assemble as fusion-incompetent immature particles and subsequently undergo conformational change leading to release of infectious virions. Flavivirus infections also produce combined 'mosaic' particles. Here, using cryo-electron tomography, we report that mosaic particles of dengue virus type 2 had glycoproteins organized into two regions of mature and immature structure. Furthermore, particles of a maturation-deficient mutant had their glycoproteins organized into two regions of immature structure with mismatching icosahedral symmetries. It is therefore apparent that the maturation-related reorganization of the flavivirus glycoproteins is not synchronized across the whole virion, but is initiated from one or more nucleation centres. Similar deviation from icosahedral symmetry might be relevant to the asymmetrical mode of genome packaging and cell entry of other viruses.

Citovat

PLEVKA, Pavel, Anthony J. BATTISTI, Jiraphan JUNJHON, Dennis C. WINKLER, Heather A. HOLDAWAY, Poonsook KEELAPANG, Nopporn SITTISOMBUT, Richard J. KUHN, Alasdair C. STEVEN and Michael G. ROSSMANN. Maturation of flaviviruses starts from one or more icosahedrally independent nucleation centres. EMBO reports. Hoboken: Wiley-Blackwell, 2011, vol. 12, No 6, p. 602-606. ISSN 1469-221X. Available from: https://dx.doi.org/10.1038/embor.2011.75.

@article{1376818,
   author = {Plevka, Pavel and Battisti, Anthony J. and Junjhon, Jiraphan and Winkler, Dennis C. and Holdaway, Heather A. and Keelapang, Poonsook and Sittisombut, Nopporn and Kuhn, Richard J. and Steven, Alasdair C. and Rossmann, Michael G.},
   article_location = {Hoboken},
   article_number = {6},
   doi = {http://dx.doi.org/10.1038/embor.2011.75},
   keywords = {dengue virus 2; maturation; mosaic; mutants; symmetry},
   language = {eng},
   issn = {1469-221X},
   journal = {EMBO reports},
   title = {Maturation of flaviviruses starts from one or more icosahedrally independent nucleation centres},
   volume = {12},
   year = {2011}
}

TY  - JOUR
ID  - 1376818
AU  - Plevka, Pavel - Battisti, Anthony J. - Junjhon, Jiraphan - Winkler, Dennis C. - Holdaway, Heather A. - Keelapang, Poonsook - Sittisombut, Nopporn - Kuhn, Richard J. - Steven, Alasdair C. - Rossmann, Michael G.
PY  - 2011
TI  - Maturation of flaviviruses starts from one or more icosahedrally independent nucleation centres
JF  - EMBO reports
VL  - 12
IS  - 6
SP  - 602-606
EP  - 602-606
PB  - Wiley-Blackwell
SN  - 1469221X
KW  - dengue virus 2
KW  - maturation
KW  - mosaic
KW  - mutants
KW  - symmetry
N2  - Flaviviruses assemble as fusion-incompetent immature particles and subsequently undergo conformational change leading to release of infectious virions. Flavivirus infections also produce combined 'mosaic' particles. Here, using cryo-electron tomography, we report that mosaic particles of dengue virus type 2 had glycoproteins organized into two regions of mature and immature structure. Furthermore, particles of a maturation-deficient mutant had their glycoproteins organized into two regions of immature structure with mismatching icosahedral symmetries. It is therefore apparent that the maturation-related reorganization of the flavivirus glycoproteins is not synchronized across the whole virion, but is initiated from one or more nucleation centres. Similar deviation from icosahedral symmetry might be relevant to the asymmetrical mode of genome packaging and cell entry of other viruses.
ER  -

PLEVKA, Pavel, Anthony J. BATTISTI, Jiraphan JUNJHON, Dennis C. WINKLER, Heather A. HOLDAWAY, Poonsook KEELAPANG, Nopporn SITTISOMBUT, Richard J. KUHN, Alasdair C. STEVEN and Michael G. ROSSMANN. Maturation of flaviviruses starts from one or more icosahedrally independent nucleation centres. \textit{EMBO reports}. Hoboken: Wiley-Blackwell, 2011, vol.~12, No~6, p.~602-606. ISSN~1469-221X. Available from: https://dx.doi.org/10.1038/embor.2011.75.

Detailed Information on Publication Record