PLEVKA, Pavel, Susan HAFENSTEIN, Lei LI, Anthony, Jr. D'ABRAMO, Susan F. COTMORE, Michael G. ROSSMANN and Peter TATTERSALL. Structure of a Packaging-Defective Mutant of Minute Virus of Mice Indicates that the Genome Is Packaged via a Pore at a 5-Fold Axis. JOURNAL OF VIROLOGY. WASHINGTON: AMER SOC MICROBIOLOGY, 2011, vol. 85, No 10, p. 4822-4827. ISSN 0022-538X. Available from: https://dx.doi.org/10.1128/JVI.02598-10. |
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@article{1376819, author = {Plevka, Pavel and Hafenstein, Susan and Li, Lei and D'Abramo, Anthony, Jr. and Cotmore, Susan F. and Rossmann, Michael G. and Tattersall, Peter}, article_location = {WASHINGTON}, article_number = {10}, doi = {http://dx.doi.org/10.1128/JVI.02598-10}, keywords = {VP1 N-TERMINUS; FUNCTIONAL IMPLICATIONS; CANINE PARVOVIRUS; DNA-REPLICATION; TYPE-2; PROTEIN; HELICASE; BINDING; CAPSIDS; VIRION}, language = {eng}, issn = {0022-538X}, journal = {JOURNAL OF VIROLOGY}, title = {Structure of a Packaging-Defective Mutant of Minute Virus of Mice Indicates that the Genome Is Packaged via a Pore at a 5-Fold Axis}, volume = {85}, year = {2011} }
TY - JOUR ID - 1376819 AU - Plevka, Pavel - Hafenstein, Susan - Li, Lei - D'Abramo, Anthony, Jr. - Cotmore, Susan F. - Rossmann, Michael G. - Tattersall, Peter PY - 2011 TI - Structure of a Packaging-Defective Mutant of Minute Virus of Mice Indicates that the Genome Is Packaged via a Pore at a 5-Fold Axis JF - JOURNAL OF VIROLOGY VL - 85 IS - 10 SP - 4822-4827 EP - 4822-4827 PB - AMER SOC MICROBIOLOGY SN - 0022538X KW - VP1 N-TERMINUS KW - FUNCTIONAL IMPLICATIONS KW - CANINE PARVOVIRUS KW - DNA-REPLICATION KW - TYPE-2 KW - PROTEIN KW - HELICASE KW - BINDING KW - CAPSIDS KW - VIRION N2 - The parvovirus minute virus of mice (MVM) packages a single copy of its linear single-stranded DNA genome into preformed capsids, in a process that is probably driven by a virus-encoded helicase. Parvoviruses have a roughly cylindrically shaped pore that surrounds each of the 12 5-fold vertices. The pore, which penetrates the virion shell, is created by the juxtaposition of 10 antiparallel beta-strands, two from each of the 5-fold-related capsid proteins. There is a bottleneck in the channel formed by the symmetry-related side chains of the leucines at position 172. We report here the X-ray crystal structure of the particles produced by a leucine-to-tryptophan mutation at position 172 and the analysis of its biochemical properties. The mutant capsid had its 5-fold channel blocked, and the particles were unable to package DNA, strongly suggesting that the 5-fold pore is the packaging portal for genome entry. ER -
PLEVKA, Pavel, Susan HAFENSTEIN, Lei LI, Anthony, Jr. D'ABRAMO, Susan F. COTMORE, Michael G. ROSSMANN and Peter TATTERSALL. Structure of a Packaging-Defective Mutant of Minute Virus of Mice Indicates that the Genome Is Packaged via a Pore at a 5-Fold Axis. \textit{JOURNAL OF VIROLOGY}. WASHINGTON: AMER SOC MICROBIOLOGY, 2011, vol.~85, No~10, p.~4822-4827. ISSN~0022-538X. Available from: https://dx.doi.org/10.1128/JVI.02598-10.
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