| ROSSMANN, Michael G., Anthony J. BATTISTI and Pavel PLEVKA. FUTURE PROSPECTS. ADVANCES IN PROTEIN CHEMISTRY AND STRUCTURAL BIOLOGY, VOL 82: RECENT ADVANCES IN ELECTRON CRYOMICROSCOPY, PT B. SAN DIEGO: ELSEVIER ACADEMIC PRESS INC, 2011, vol. 82, neuvedeno, p. 101-121. ISSN 1876-1623. Available from: https://dx.doi.org/10.1016/B978-0-12-386507-6.00005-1. |
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@article{1376825,
author = {Rossmann, Michael G. and Battisti, Anthony J. and Plevka, Pavel},
article_location = {SAN DIEGO},
article_number = {neuvedeno},
doi = {http://dx.doi.org/10.1016/B978-0-12-386507-6.00005-1},
keywords = {3-DIMENSIONAL FOURIER SYNTHESIS; X-RAY ANALYSIS; CRYOELECTRON TOMOGRAPHY; ELECTRON-MICROSCOPY; ATOMIC MODELS; NEUTRALIZING ANTIBODY; INTERATOMIC DISTANCES; MOLECULAR-STRUCTURE; CRYSTAL-STRUCTURES; CANINE PARVOVIRUS},
language = {eng},
issn = {1876-1623},
journal = {ADVANCES IN PROTEIN CHEMISTRY AND STRUCTURAL BIOLOGY, VOL 82: RECENT ADVANCES IN ELECTRON CRYOMICROSCOPY, PT B},
title = {FUTURE PROSPECTS},
volume = {82},
year = {2011}
}
TY - JOUR
ID - 1376825
AU - Rossmann, Michael G. - Battisti, Anthony J. - Plevka, Pavel
PY - 2011
TI - FUTURE PROSPECTS
JF - ADVANCES IN PROTEIN CHEMISTRY AND STRUCTURAL BIOLOGY, VOL 82: RECENT ADVANCES IN ELECTRON CRYOMICROSCOPY, PT B
VL - 82
IS - neuvedeno
SP - 101-121
EP - 101-121
PB - ELSEVIER ACADEMIC PRESS INC
SN - 18761623
KW - 3-DIMENSIONAL FOURIER SYNTHESIS
KW - X-RAY ANALYSIS
KW - CRYOELECTRON TOMOGRAPHY
KW - ELECTRON-MICROSCOPY
KW - ATOMIC MODELS
KW - NEUTRALIZING ANTIBODY
KW - INTERATOMIC DISTANCES
KW - MOLECULAR-STRUCTURE
KW - CRYSTAL-STRUCTURES
KW - CANINE PARVOVIRUS
N2 - Cryo-electron microscopy (cryo-EM) in combination with single-particle analysis has begun to complement crystallography in the study of large macromolecules at near-atomic resolution. Furthermore, advances in cryo-electron tomography have made possible the study of macromolecules within their cellular environment. Single-particle and tomographic studies will become even more useful when technologies for improving the signal-to-noise ratio such as direct electron detectors and phase plates become widely available. Automated image acquisition has significantly reduced the time and effort required to determine the structures of macromolecular assemblies. As a result, the number of structures determined by cryo-EM is growing exponentially. However, there is an urgent need for improved criteria for validating both the reconstruction process and the atomic models derived from cryo-EM data. Another major challenge will be mitigating the effects of anisotropy caused by the missing wedge and the excessively low signal-to-noise ratio for tomographic data. Parallels between the development of macromolecular crystallography and cryo-EM have been used to tentatively predict the future of cryo-EM.
ER -
ROSSMANN, Michael G., Anthony J. BATTISTI and Pavel PLEVKA. FUTURE PROSPECTS. \textit{ADVANCES IN PROTEIN CHEMISTRY AND STRUCTURAL BIOLOGY, VOL 82: RECENT ADVANCES IN ELECTRON CRYOMICROSCOPY, PT B}. SAN DIEGO: ELSEVIER ACADEMIC PRESS INC, 2011, vol.~82, neuvedeno, p.~101-121. ISSN~1876-1623. Available from: https://dx.doi.org/10.1016/B978-0-12-386507-6.00005-1.
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