| KAUFMANN, Baerbel, Pavel PLEVKA, Richard J. KUHN and Michael G. ROSSMANN. Crystallization and preliminary X-ray diffraction analysis of West Nile virus. Acta crystallographica. Section F Structural biology and crystallization communications. HOBOKEN: WILEY-BLACKWELL, 2010, vol. 66, May, p. 558-562. ISSN 1744-3091. Available from: https://dx.doi.org/10.1107/S1744309110009899. |
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@article{1376892,
author = {Kaufmann, Baerbel and Plevka, Pavel and Kuhn, Richard J. and Rossmann, Michael G.},
article_location = {HOBOKEN},
article_number = {May},
doi = {http://dx.doi.org/10.1107/S1744309110009899},
keywords = {DENGUE VIRUS; ENVELOPE GLYCOPROTEIN; MEMBRANE-FUSION; VAPOR-DIFFUSION; PROTEIN; MATURATION; CRYSTALS},
language = {eng},
issn = {1744-3091},
journal = {Acta crystallographica. Section F Structural biology and crystallization communications},
title = {Crystallization and preliminary X-ray diffraction analysis of West Nile virus},
volume = {66},
year = {2010}
}
TY - JOUR
ID - 1376892
AU - Kaufmann, Baerbel - Plevka, Pavel - Kuhn, Richard J. - Rossmann, Michael G.
PY - 2010
TI - Crystallization and preliminary X-ray diffraction analysis of West Nile virus
JF - Acta crystallographica. Section F Structural biology and crystallization communications
VL - 66
IS - May
SP - 558-562
EP - 558-562
PB - WILEY-BLACKWELL
SN - 17443091
KW - DENGUE VIRUS
KW - ENVELOPE GLYCOPROTEIN
KW - MEMBRANE-FUSION
KW - VAPOR-DIFFUSION
KW - PROTEIN
KW - MATURATION
KW - CRYSTALS
N2 - West Nile virus, a human pathogen, is closely related to other medically important flaviviruses of global impact such as dengue virus. The infectious virus was purified from cell culture using polyethylene glycol (PEG) precipitation and density-gradient centrifugation. Thin amorphously shaped crystals of the lipid-enveloped virus were grown in quartz capillaries equilibrated by vapor diffusion. Crystal diffraction extended at best to a resolution of about 25 angstrom using synchrotron radiation. A preliminary analysis of the diffraction images indicated that the crystals had unit-cell parameters a similar or equal to b similar or equal to 480 angstrom, gamma = 120 degrees, suggesting a tight hexagonal packing of one virus particle per unit cell.
ER -
KAUFMANN, Baerbel, Pavel PLEVKA, Richard J. KUHN and Michael G. ROSSMANN. Crystallization and preliminary X-ray diffraction analysis of West Nile virus. \textit{Acta crystallographica. Section F Structural biology and crystallization communications}. HOBOKEN: WILEY-BLACKWELL, 2010, vol.~66, May, p.~558-562. ISSN~1744-3091. Available from: https://dx.doi.org/10.1107/S1744309110009899.
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