PLEVKA, Pavel, Kaspars TARS a Lars LILJAS. Structure and stability of icosahedral particles of a covalent coat protein dimer of bacteriophage MS2. Protein Science. HOBOKEN: JOHN WILEY & SONS INC, 2009, roč. 18, č. 8, s. 1653-1661. ISSN 0961-8368. Dostupné z: https://dx.doi.org/10.1002/pro.184. |
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@article{1376896, author = {Plevka, Pavel and Tars, Kaspars and Liljas, Lars}, article_location = {HOBOKEN}, article_number = {8}, doi = {http://dx.doi.org/10.1002/pro.184}, keywords = {virus; structure; assembly; stability}, language = {eng}, issn = {0961-8368}, journal = {Protein Science}, title = {Structure and stability of icosahedral particles of a covalent coat protein dimer of bacteriophage MS2}, volume = {18}, year = {2009} }
TY - JOUR ID - 1376896 AU - Plevka, Pavel - Tars, Kaspars - Liljas, Lars PY - 2009 TI - Structure and stability of icosahedral particles of a covalent coat protein dimer of bacteriophage MS2 JF - Protein Science VL - 18 IS - 8 SP - 1653-1661 EP - 1653-1661 PB - JOHN WILEY & SONS INC SN - 09618368 KW - virus KW - structure KW - assembly KW - stability N2 - Particles formed by the bacteriophage MS2 coat protein mutants with insertions in their surface loops induce a strong immune response against the inserted epitopes. The covalent dimers created by fusion of two copies of the coat protein gene are more tolerant to various insertions into the surface loops than the single subunits. We determined a 4.7-angstrom resolution crystal structure of an icosahedral particle assembled from covalent dimers and compared its stability with wild-type virions. The structure resembled the wild-type virion except for the intersubunit linker regions. The covalent dimer orientation was random with respect to both icosahedral twofold and quasi-twofold symmetry axes. A fraction of the particles was unstable in phosphate buffer because of assembly defects. Our results provide a structural background for design of modified covalent coat protein dimer subunits for use in immunization. ER -
PLEVKA, Pavel, Kaspars TARS a Lars LILJAS. Structure and stability of icosahedral particles of a covalent coat protein dimer of bacteriophage MS2. \textit{Protein Science}. HOBOKEN: JOHN WILEY \&{}amp; SONS INC, 2009, roč.~18, č.~8, s.~1653-1661. ISSN~0961-8368. Dostupné z: https://dx.doi.org/10.1002/pro.184.
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