Kinetic Properties of Pyruvate Ferredoxin Oxidoreductase of
Intestinal Sulfate-Reducing Bacteria Desulfovibrio piger Vib-7
and Desulfomicrobium sp. Rod-9

J 2015

Kinetic Properties of Pyruvate Ferredoxin Oxidoreductase of Intestinal Sulfate-Reducing Bacteria Desulfovibrio piger Vib-7 and Desulfomicrobium sp. Rod-9

KUSHKEVYCH, Ivan

Basic information

Original name

Kinetic Properties of Pyruvate Ferredoxin Oxidoreductase of Intestinal Sulfate-Reducing Bacteria Desulfovibrio piger Vib-7 and Desulfomicrobium sp. Rod-9

Authors

KUSHKEVYCH, Ivan

Edition

Polish Journal of Microbiology, Warsaw, POLSKIE TOWARZYSTWO MIKROBIOLOGOW-POLISH SOCIETY OF MICROBIOLOGISTS, 2015, 1733-1331

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Poland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Full Text

Impact factor

Impact factor: 0.750

Organization unit

Faculty of Science

UT WoS

000357361800005

Keywords in English

kinetic analysis; inflammatory bowel diseases; pyruvate ferredoxin oxidoreductase; sulfate-reducing bacteria

Abstract

V originále

Intestinal sulfate-reducing bacteria reduce sulfate ions to hydrogen sulfide causing inflammatory bowel diseases of humans and animals. The bacteria consume lactate as electron donor which is oxidized to acetate via pyruvate in process of the dissimilatory sulfate reduction. Pyruvate-ferredoxin oxidoreductase activity and the kinetic properties of the enzyme from intestinal sulfate-reducing bacteria Desulfovibrio piger and Desulfomicrobium sp. have never been well-characterized and have not been yet studied. In this paper we present for the first time the specific activity of pyruvate-ferredoxin oxidoreductase and the kinetic properties of the enzyme in cell-free extracts of both D. piger Vib-7 and Desulfomicrobium sp. Rod-9 intestinal bacterial strains. Microbiological, biochemical, biophysical and statistical methods were used in this work. The optimal temperature (+35°C) and pH 8.5 for enzyme reaction were determined. The spectral analysis of the purified pyruvate-ferredoxin oxidoreductase from the cell-free extracts was demonstrated. Analysis of the kinetic properties of the studied enzyme was carried out. Initial (instantaneous) reaction velocity (V0), maximum amount of the product of reaction (Pmax), the reaction time (half saturation period) and maximum velocity of the pyruvate-ferredoxin oxidoreductase reaction (Vmax) were defined. Michaelis constants (Km) of the enzyme reaction were calculated for both intestinal bacterial strains. The studies of the kinetic enzyme properties in the intestinal sulfate-reducing bacteria strains in detail can be prospects for clarifying the etiological role of these bacteria in the development of inflammatory bowel diseases

Citovat

KUSHKEVYCH, Ivan. Kinetic Properties of Pyruvate Ferredoxin Oxidoreductase of Intestinal Sulfate-Reducing Bacteria Desulfovibrio piger Vib-7 and Desulfomicrobium sp. Rod-9. Polish Journal of Microbiology. Warsaw: POLSKIE TOWARZYSTWO MIKROBIOLOGOW-POLISH SOCIETY OF MICROBIOLOGISTS, 2015, vol. 64, No 2, p. 107-114. ISSN 1733-1331.

@article{1377005,
   author = {Kushkevych, Ivan},
   article_location = {Warsaw},
   article_number = {2},
   keywords = {kinetic analysis; inflammatory bowel diseases; pyruvate ferredoxin oxidoreductase; sulfate-reducing bacteria},
   language = {eng},
   issn = {1733-1331},
   journal = {Polish Journal of Microbiology},
   note = {Nelze přenášet do RIV - doc. Kushkevych nemá na WoS afiliaci na MU, nýbrž na NAAS Ukraine, Inst Anim Biol, Lvov, Ukraine},
   title = {Kinetic Properties of Pyruvate Ferredoxin Oxidoreductase of Intestinal Sulfate-Reducing Bacteria Desulfovibrio piger Vib-7 and Desulfomicrobium sp. Rod-9},
   url = {http://www.pjm.microbiology.pl/archive/vol6422015107.pdf},
   volume = {64},
   year = {2015}
}

TY  - JOUR
ID  - 1377005
AU  - Kushkevych, Ivan
PY  - 2015
TI  - Kinetic Properties of Pyruvate Ferredoxin Oxidoreductase of Intestinal Sulfate-Reducing Bacteria Desulfovibrio piger Vib-7 and Desulfomicrobium sp. Rod-9
JF  - Polish Journal of Microbiology
VL  - 64
IS  - 2
SP  - 107-114
EP  - 107-114
PB  - POLSKIE TOWARZYSTWO MIKROBIOLOGOW-POLISH SOCIETY OF MICROBIOLOGISTS
SN  - 17331331
N1  - Nelze přenášet do RIV - doc. Kushkevych nemá na WoS afiliaci na MU, nýbrž na NAAS Ukraine, Inst Anim Biol, Lvov, Ukraine
KW  - kinetic analysis
KW  - inflammatory bowel diseases
KW  - pyruvate ferredoxin oxidoreductase
KW  - sulfate-reducing bacteria
UR  - http://www.pjm.microbiology.pl/archive/vol6422015107.pdf
N2  - Intestinal sulfate-reducing bacteria reduce sulfate ions to hydrogen sulfide causing inflammatory bowel diseases of humans and animals. The bacteria consume lactate as electron donor which is oxidized to acetate via pyruvate in process of the dissimilatory sulfate reduction. Pyruvate-ferredoxin oxidoreductase activity and the kinetic properties of the enzyme from intestinal sulfate-reducing bacteria Desulfovibrio piger and Desulfomicrobium sp. have never been well-characterized and have not been yet studied. In this paper we present for the first time the specific activity of pyruvate-ferredoxin oxidoreductase and the kinetic properties of the enzyme in cell-free extracts of both D. piger Vib-7 and Desulfomicrobium sp. Rod-9 intestinal bacterial strains. Microbiological, biochemical, biophysical and statistical methods were used in this work. The optimal temperature (+35°C) and pH 8.5 for enzyme reaction were determined. The spectral analysis of the purified pyruvate-ferredoxin oxidoreductase from the cell-free extracts was demonstrated. Analysis of the kinetic properties of the studied enzyme was carried out. Initial (instantaneous) reaction velocity (V0), maximum amount of the product of reaction (Pmax), the reaction time (half saturation period) and maximum velocity of the pyruvate-ferredoxin oxidoreductase reaction (Vmax) were defined. Michaelis constants (Km) of the enzyme reaction were calculated for both intestinal bacterial strains. The studies of the kinetic enzyme properties in the intestinal sulfate-reducing bacteria strains in detail can be prospects for clarifying the etiological role of these bacteria in the development of inflammatory bowel diseases
ER  -

KUSHKEVYCH, Ivan. Kinetic Properties of Pyruvate Ferredoxin Oxidoreductase of Intestinal Sulfate-Reducing Bacteria Desulfovibrio piger Vib-7 and Desulfomicrobium sp. Rod-9. \textit{Polish Journal of Microbiology}. Warsaw: POLSKIE TOWARZYSTWO MIKROBIOLOGOW-POLISH SOCIETY OF MICROBIOLOGISTS, 2015, vol.~64, No~2, p.~107-114. ISSN~1733-1331.

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