Activity and kinetic properties of phosphotransacetylase from intestinal sulfate-reducing bacteria
KUSHKEVYCH, Ivan. Activity and kinetic properties of phosphotransacetylase from intestinal sulfate-reducing bacteria. ACTA BIOCHIMICA POLONICA. 2015, vol. 2015, No 62, p. 103-108. ISSN 0001-527X. Available from: https://dx.doi.org/10.18388/abp.2014_845. |
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Basic information | |
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Original name | Activity and kinetic properties of phosphotransacetylase from intestinal sulfate-reducing bacteria |
Authors | KUSHKEVYCH, Ivan. |
Edition | ACTA BIOCHIMICA POLONICA, 2015, 0001-527X. |
Other information | |
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Original language | English |
Type of outcome | Article in a journal |
Field of Study | 10600 1.6 Biological sciences |
Country of publisher | Poland |
Confidentiality degree | is not subject to a state or trade secret |
WWW | URL |
Impact factor | Impact factor: 1.187 |
Organization unit | Faculty of Science |
Doi | http://dx.doi.org/10.18388/abp.2014_845 |
UT WoS | 000351891600014 |
Keywords in English | sulfate-reducing bacteria; phosphotransacetylase; kinetic analysis; inflammatory bowel diseases |
Tags | NZ |
Changed by | Changed by: Mgr. Marie Šípková, DiS., učo 437722. Changed: 6/3/2020 09:00. |
Abstract |
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Phosphotransacetylase activity and the kinetic properties of the enzyme from intestinal sulfate-reducing bacteria Desulfovibrio piger and Desulfomicrobium sp. has never been well-characterized and has not been studied yet. In this paper, the specific activity of phosphotransacetylase and the kinetic properties of the enzyme in cell-free extracts of both D. piger Vib-7 and Desulfomicrobium sp. Rod-9 intestinal bacterial strains were presented at the first time. The microbiological, biochemical, biophysical and statistical methods in this work were used. The optimal temperature and pH for enzyme reaction was determined. Analysis of the kinetic properties of the studied enzyme was carried out. Initial (instantaneous) reaction velocity (V-0), maximum amount of the product of reaction (P-max), the reaction time (half saturation period, tau) and maximum velocity of the phosphotransacetylase reaction (V-max) were defined. Michaelis constants (K-m) of the enzyme reaction (3.36 +/- 0.35 mM for D. piger Vib-7, 5.97 +/- 0.62 mM for Desulfomicrobium sp. Rod-9) were calculated. The studies of the phosphotransacetylase in the process of dissimilatory sulfate reduction and kinetic properties of this enzyme in intestinal sulfate-reducing bacteria, their production of acetate in detail can be perspective for clarification of their etiological role in the development of the humans and animals bowel diseases. These studies might help in predicting the development of diseases of the gastrointestinal tract, by providing further details on the etiology of bowel diseases which are very important for the clinical diagnosis of these disease types. |
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