Activity of Na+/K+-activated Mg2+-dependent ATP hydrolase in
the cell-free extracts of the sulfate-reducing bacteria
Desulfovibrio piger Vib-7 and Desulfomicrobium sp. Rod-9

J 2015

Activity of Na+/K+-activated Mg2+-dependent ATP hydrolase in the cell-free extracts of the sulfate-reducing bacteria Desulfovibrio piger Vib-7 and Desulfomicrobium sp. Rod-9

KUSHKEVYCH, Ivan, Roman FAFULA, Tomáš PARÁK and Milan BARTOŠ

Basic information

Original name

Activity of Na+/K+-activated Mg2+-dependent ATP hydrolase in the cell-free extracts of the sulfate-reducing bacteria Desulfovibrio piger Vib-7 and Desulfomicrobium sp. Rod-9

Authors

KUSHKEVYCH, Ivan, Roman FAFULA, Tomáš PARÁK and Milan BARTOŠ

Edition

ACTA VETERINARIA BRNO, 2015, 0001-7213

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 0.442

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.2754/avb201585010003

UT WoS

000351657800001

Keywords in English

ATPase activity; hydrogen sulfide; intestinal microbiocenosis; bowel diseases; ulcerative colitis

Abstract

V originále

The aim of our work was to study Na+/K+-activated Mg2+-dependent ATPase activity in cell-free extracts of the sulfate-reducing bacteria Desulfovibrio piger Vib-7 and Desulfomicrobium sp. Rod-9 isolated from the human large intestine, and to carry out the kinetic analysis of the enzyme reaction. The maximum ATPase activity for both bacterial strains at +35C was determined. The highest activities of the studied enzyme in the cell-free extracts of D. piger Vib-7 at pH 7.0 and Desulfomicrobium sp. Rod-9 at pH 6.5 were measured. Based on experimental data, the analysis of kinetic properties of the ATP-hydrolase reaction by the studied bacteria was carried out. The enzyme activity, initial (instantaneous) reaction rate (V0) and maximum rate of the ATPase reaction (Vmax) was significantly higher in D. piger Vib-7 cells than in Desulfomicrobium sp. Rod-9. Michaelis constants (Km) of the enzyme reaction for both bacterial strains were determined.

Citovat

KUSHKEVYCH, Ivan, Roman FAFULA, Tomáš PARÁK and Milan BARTOŠ. Activity of Na+/K+-activated Mg2+-dependent ATP hydrolase in the cell-free extracts of the sulfate-reducing bacteria Desulfovibrio piger Vib-7 and Desulfomicrobium sp. Rod-9. ACTA VETERINARIA BRNO. 2015, vol. 2015, No 84, p. 3-12. ISSN 0001-7213. Available from: https://dx.doi.org/10.2754/avb201585010003.

@article{1377098,
   author = {Kushkevych, Ivan and Fafula, Roman and Parák, Tomáš and Bartoš, Milan},
   article_number = {84},
   doi = {http://dx.doi.org/10.2754/avb201585010003},
   keywords = {ATPase activity; hydrogen sulfide; intestinal microbiocenosis; bowel diseases; ulcerative colitis},
   language = {eng},
   issn = {0001-7213},
   journal = {ACTA VETERINARIA BRNO},
   note = {Doc. Kushkevych ani jeho spoluautoři nemají na WoS afiliaci na MU, nelze přenášet do RIV.},
   title = {Activity of Na+/K+-activated Mg2+-dependent ATP hydrolase in the cell-free extracts of the sulfate-reducing bacteria Desulfovibrio piger Vib-7 and Desulfomicrobium sp. Rod-9},
   url = {http://dx.doi.org/10.2754/avb201585010003},
   volume = {2015},
   year = {2015}
}

TY  - JOUR
ID  - 1377098
AU  - Kushkevych, Ivan - Fafula, Roman - Parák, Tomáš - Bartoš, Milan
PY  - 2015
TI  - Activity of Na+/K+-activated Mg2+-dependent ATP hydrolase in the cell-free extracts of the sulfate-reducing bacteria Desulfovibrio piger Vib-7 and Desulfomicrobium sp. Rod-9
JF  - ACTA VETERINARIA BRNO
VL  - 2015
IS  - 84
SP  - 3-12
EP  - 3-12
SN  - 00017213
N1  - Doc. Kushkevych ani jeho spoluautoři nemají na WoS afiliaci na MU, nelze přenášet do RIV.
KW  - ATPase activity
KW  - hydrogen sulfide
KW  - intestinal microbiocenosis
KW  - bowel diseases
KW  - ulcerative colitis
UR  - http://dx.doi.org/10.2754/avb201585010003
L2  - http://dx.doi.org/10.2754/avb201585010003
N2  - The aim of our work was to study Na+/K+-activated Mg2+-dependent ATPase activity in cell-free extracts of the sulfate-reducing bacteria Desulfovibrio piger Vib-7 and Desulfomicrobium sp. Rod-9 isolated from the human large intestine, and to carry out the kinetic analysis of the enzyme reaction. The maximum ATPase activity for both bacterial strains at +35C was determined. The highest activities of the studied enzyme in the cell-free extracts of D. piger Vib-7 at pH 7.0 and Desulfomicrobium sp. Rod-9 at pH 6.5 were measured. Based on experimental data, the analysis of kinetic properties of the ATP-hydrolase reaction by the studied bacteria was carried out. The enzyme activity, initial (instantaneous) reaction rate (V0) and maximum rate of the ATPase reaction (Vmax) was significantly higher in D. piger Vib-7 cells than in Desulfomicrobium sp. Rod-9. Michaelis constants (Km) of the enzyme reaction for both bacterial strains were determined.
ER  -

KUSHKEVYCH, Ivan, Roman FAFULA, Tomáš PARÁK and Milan BARTOŠ. Activity of Na+/K+-activated Mg2+-dependent ATP hydrolase in the cell-free extracts of the sulfate-reducing bacteria Desulfovibrio piger Vib-7 and Desulfomicrobium sp. Rod-9. \textit{ACTA VETERINARIA BRNO}. 2015, vol.~2015, No~84, p.~3-12. ISSN~0001-7213. Available from: https://dx.doi.org/10.2754/avb201585010003.

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