KUSHKEVYCH, Ivan, Roman FAFULA, Tomáš PARÁK and Milan BARTOŠ. Activity of Na+/K+-activated Mg2+-dependent ATP hydrolase in the cell-free extracts of the sulfate-reducing bacteria Desulfovibrio piger Vib-7 and Desulfomicrobium sp. Rod-9. ACTA VETERINARIA BRNO. 2015, vol. 2015, No 84, p. 3-12. ISSN 0001-7213. Available from: https://dx.doi.org/10.2754/avb201585010003.
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Basic information
Original name Activity of Na+/K+-activated Mg2+-dependent ATP hydrolase in the cell-free extracts of the sulfate-reducing bacteria Desulfovibrio piger Vib-7 and Desulfomicrobium sp. Rod-9
Authors KUSHKEVYCH, Ivan, Roman FAFULA, Tomáš PARÁK and Milan BARTOŠ.
Edition ACTA VETERINARIA BRNO, 2015, 0001-7213.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10600 1.6 Biological sciences
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 0.442
Organization unit Faculty of Science
Doi http://dx.doi.org/10.2754/avb201585010003
UT WoS 000351657800001
Keywords in English ATPase activity; hydrogen sulfide; intestinal microbiocenosis; bowel diseases; ulcerative colitis
Tags NZ
Changed by Changed by: Mgr. Marie Šípková, DiS., učo 437722. Changed: 6/3/2020 08:58.
Abstract
The aim of our work was to study Na+/K+-activated Mg2+-dependent ATPase activity in cell-free extracts of the sulfate-reducing bacteria Desulfovibrio piger Vib-7 and Desulfomicrobium sp. Rod-9 isolated from the human large intestine, and to carry out the kinetic analysis of the enzyme reaction. The maximum ATPase activity for both bacterial strains at +35C was determined. The highest activities of the studied enzyme in the cell-free extracts of D. piger Vib-7 at pH 7.0 and Desulfomicrobium sp. Rod-9 at pH 6.5 were measured. Based on experimental data, the analysis of kinetic properties of the ATP-hydrolase reaction by the studied bacteria was carried out. The enzyme activity, initial (instantaneous) reaction rate (V0) and maximum rate of the ATPase reaction (Vmax) was significantly higher in D. piger Vib-7 cells than in Desulfomicrobium sp. Rod-9. Michaelis constants (Km) of the enzyme reaction for both bacterial strains were determined.
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