Podrobný výpis o publikaci

KRANC, Wiesława, Joanna BUDNA, Adrian CHACHUŁA, Sylwia BORYS, Artur BRYJA, Marta RYBSKA, Sylwia CIESIÓŁKA, Eva SUMELKA, Michal JEŠETA, Klaus P. BRÜSSOW, Dorota BUKOWSKA, Paweł ANTOSIK, Małgorzata BRUSKA, Michał NOWICKI, Maciej ZABEL a Bartosz KEMPISTY. “Cell Migration” Is the Ontology Group Differentially Expressed in Porcine Oocytes Before and After In Vitro Maturation: A Microarray Approach. DNA and Cell Biology. New York: Mary Ann Liebert Inc., 2017, roč. 36, č. 4, s. 273-282. ISSN 1044-5498. Dostupné z: https://dx.doi.org/10.1089/dna.2016.3425.

Další formáty: BibTeX LaTeX RIS

TY  - JOUR
ID  - 1378656
AU  - Kranc, Wiesława - Budna, Joanna - Chachuła, Adrian - Borys, Sylwia - Bryja, Artur - Rybska, Marta - Ciesiółka, Sylwia - Sumelka, Eva - Ješeta, Michal - Brüssow, Klaus P. - Bukowska, Dorota - Antosik, Paweł - Bruska, Małgorzata - Nowicki, Michał - Zabel, Maciej - Kempisty, Bartosz
PY  - 2017
TI  - “Cell Migration” Is the Ontology Group Differentially Expressed in Porcine Oocytes Before and After In Vitro Maturation: A Microarray Approach
JF  - DNA and Cell Biology
VL  - 36
IS  - 4
SP  - 273-282
EP  - 273-282
PB  - Mary Ann Liebert Inc.
SN  - 10445498
KW  - pig
KW  - oocyte
KW  - microarray
KW  - cell migration
N2  - Maturation of cumulus–oocyte complexes (COCs) is crucial for further successful monospermic fertilization, embryo growth, and implantation. All these events are accompanied by proliferation and differentiation of cumulus cells. The migration of COCs to the oviduct after ovulation and the interaction between female gametes and/or embryos with maternal tissues are still poorly recognized on the molecular level. This study was aimed to first demonstrate the mRNA expression profile of cell migration markers during different stages of porcine oocytes maturation and developmental capability in vitro. The COCs were collected from a total of 45 pubertal crossbred Landrace gilts, brilliant cresyl blue (BCB) stained, and analyzed before (n = 150) or after (n = 150) in vitro maturation (IVM). Using the Affymetrix Porcine Gene 1.1 ST Array, the expression profile of 12,258 porcine transcripts was examined. We found nine genes involved in cell migration mechanisms, that is, PLD1, KIT, LAMA2, MAP3K1, VEGFA, TGFBR3, INSR, TPM1, and RTN4. These genes were upregulated in porcine oocytes before IVM as compared with post-IVM expression analysis. Moreover, important mechanisms of biological interaction between VEGFA–KIT and VEGFA–INSR were also observed. The upregulation and/or downregulation of selected mRNAs expression after microarray assays was checked and approved by real-time quantitative polymerase chain reaction. We suggest that several genes, including LAMA2 or TPM1, encode proteins participating in the formation of the oocyte’s protein architecture such as microtubules and kinetochore reorganization. As the expression of all migration regulatory genes investigated in this study was significantly upregulated in oocytes before IVM, we conclude that they may contribute to the maturational capability of porcine oocytes. However, migration potency of COCs is not accompanied by achievement of the MII stage by porcine oocytes in vitro. The investigated genes such as PLD1, KIT, LAMA2, MAP3K1, VEGFA, TGFBR3, INSR, TPM1, and RTN4 may be recognized as a new marker of porcine oocytes maturational competence during in vitro culture.
ER  -

Základní údaje
Originální název	“Cell Migration” Is the Ontology Group Differentially Expressed in Porcine Oocytes Before and After In Vitro Maturation: A Microarray Approach
Autoři	KRANC, Wiesława (616 Polsko), Joanna BUDNA (616 Polsko), Adrian CHACHUŁA (616 Polsko), Sylwia BORYS (616 Polsko), Artur BRYJA (616 Polsko), Marta RYBSKA (616 Polsko), Sylwia CIESIÓŁKA (616 Polsko), Eva SUMELKA (616 Polsko), Michal JEŠETA (203 Česká republika, garant, domácí), Klaus P. BRÜSSOW (616 Polsko), Dorota BUKOWSKA (616 Polsko), Paweł ANTOSIK (616 Polsko), Małgorzata BRUSKA (616 Polsko), Michał NOWICKI (616 Polsko), Maciej ZABEL (616 Polsko) a Bartosz KEMPISTY (616 Polsko).
Vydání	DNA and Cell Biology, New York, Mary Ann Liebert Inc. 2017, 1044-5498.

Další údaje
Originální jazyk	angličtina
Typ výsledku	Článek v odborném periodiku
Obor	10608 Biochemistry and molecular biology
Stát vydavatele	Spojené státy
Utajení	není předmětem státního či obchodního tajemství
Impakt faktor	Impact factor: 2.634
Kód RIV	RIV/00216224:14110/17:00096496
Organizační jednotka	Lékařská fakulta
Doi	http://dx.doi.org/10.1089/dna.2016.3425
UT WoS	000398466900005
Klíčová slova anglicky	pig; oocyte; microarray; cell migration
Štítky	EL OK
Příznaky	Mezinárodní význam, Recenzováno
Změnil	Změnila: Soňa Böhmová, učo 232884. Změněno: 21. 3. 2018 13:29.

Anotace
Maturation of cumulus–oocyte complexes (COCs) is crucial for further successful monospermic fertilization, embryo growth, and implantation. All these events are accompanied by proliferation and differentiation of cumulus cells. The migration of COCs to the oviduct after ovulation and the interaction between female gametes and/or embryos with maternal tissues are still poorly recognized on the molecular level. This study was aimed to first demonstrate the mRNA expression profile of cell migration markers during different stages of porcine oocytes maturation and developmental capability in vitro. The COCs were collected from a total of 45 pubertal crossbred Landrace gilts, brilliant cresyl blue (BCB) stained, and analyzed before (n = 150) or after (n = 150) in vitro maturation (IVM). Using the Affymetrix Porcine Gene 1.1 ST Array, the expression profile of 12,258 porcine transcripts was examined. We found nine genes involved in cell migration mechanisms, that is, PLD1, KIT, LAMA2, MAP3K1, VEGFA, TGFBR3, INSR, TPM1, and RTN4. These genes were upregulated in porcine oocytes before IVM as compared with post-IVM expression analysis. Moreover, important mechanisms of biological interaction between VEGFA–KIT and VEGFA–INSR were also observed. The upregulation and/or downregulation of selected mRNAs expression after microarray assays was checked and approved by real-time quantitative polymerase chain reaction. We suggest that several genes, including LAMA2 or TPM1, encode proteins participating in the formation of the oocyte’s protein architecture such as microtubules and kinetochore reorganization. As the expression of all migration regulatory genes investigated in this study was significantly upregulated in oocytes before IVM, we conclude that they may contribute to the maturational capability of porcine oocytes. However, migration potency of COCs is not accompanied by achievement of the MII stage by porcine oocytes in vitro. The investigated genes such as PLD1, KIT, LAMA2, MAP3K1, VEGFA, TGFBR3, INSR, TPM1, and RTN4 may be recognized as a new marker of porcine oocytes maturational competence during in vitro culture.

Anotace

Maturation of cumulus–oocyte complexes (COCs) is crucial for further successful monospermic fertilization, embryo growth, and implantation. All these events are accompanied by proliferation and differentiation of cumulus cells. The migration of COCs to the oviduct after ovulation and the interaction between female gametes and/or embryos with maternal tissues are still poorly recognized on the molecular level. This study was aimed to first demonstrate the mRNA expression profile of cell migration markers during different stages of porcine oocytes maturation and developmental capability in vitro. The COCs were collected from a total of 45 pubertal crossbred Landrace gilts, brilliant cresyl blue (BCB) stained, and analyzed before (n = 150) or after (n = 150) in vitro maturation (IVM). Using the Affymetrix Porcine Gene 1.1 ST Array, the expression profile of 12,258 porcine transcripts was examined. We found nine genes involved in cell migration mechanisms, that is, PLD1, KIT, LAMA2, MAP3K1, VEGFA, TGFBR3, INSR, TPM1, and RTN4. These genes were upregulated in porcine oocytes before IVM as compared with post-IVM expression analysis. Moreover, important mechanisms of biological interaction between VEGFA–KIT and VEGFA–INSR were also observed. The upregulation and/or downregulation of selected mRNAs expression after microarray assays was checked and approved by real-time quantitative polymerase chain reaction. We suggest that several genes, including LAMA2 or TPM1, encode proteins participating in the formation of the oocyte’s protein architecture such as microtubules and kinetochore reorganization. As the expression of all migration regulatory genes investigated in this study was significantly upregulated in oocytes before IVM, we conclude that they may contribute to the maturational capability of porcine oocytes. However, migration potency of COCs is not accompanied by achievement of the MII stage by porcine oocytes in vitro. The investigated genes such as PLD1, KIT, LAMA2, MAP3K1, VEGFA, TGFBR3, INSR, TPM1, and RTN4 may be recognized as a new marker of porcine oocytes maturational competence during in vitro culture.