Nicotine at clinically relevant concentrations affects atrial
inward rectifier potassium current sensitive to acetylcholine

J 2017

Nicotine at clinically relevant concentrations affects atrial inward rectifier potassium current sensitive to acetylcholine

BÉBAROVÁ, Markéta, Peter MATEJOVIČ, Olga ŠVECOVÁ, Roman KULA, Milena ŠIMURDOVÁ et. al.

Basic information

Original name

Nicotine at clinically relevant concentrations affects atrial inward rectifier potassium current sensitive to acetylcholine

Authors

BÉBAROVÁ, Markéta (203 Czech Republic, guarantor, belonging to the institution), Peter MATEJOVIČ (203 Czech Republic, belonging to the institution), Olga ŠVECOVÁ (643 Russian Federation, belonging to the institution), Roman KULA (203 Czech Republic, belonging to the institution), Milena ŠIMURDOVÁ (203 Czech Republic, belonging to the institution) and Jiří ŠIMURDA (203 Czech Republic, belonging to the institution)

Edition

Naunyn-Schmiedeberg's Archives of Pharmacology, New York, Springer, 2017, 0028-1298

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30104 Pharmacology and pharmacy

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 2.238

RIV identification code

RIV/00216224:14110/17:00096591

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1007/s00210-017-1341-z

UT WoS

000399170600003

Keywords in English

Acetylcholine-sensitive; Dual effect; Inward rectifier; Nicotine

Abstract

V originále

Nicotine abuse is associated with variety of diseases including arrhythmias, most often atrial fibrillation (AF). Altered inward rectifier potassium currents including acetylcholine-sensitive current IK(Ach) are known to be related to AF pathogenesis. Since relevant data are missing, we aimed to investigate IK(Ach) changes at clinically relevant concentrations of nicotine. Experiments were performed by the whole cell patch clamp technique at 23 +/- 1 °C on isolated rat atrial myocytes. Nicotine was applied at following concentrations: 4, 40 and 400 nM; ethanol at 20 mM (0.09%). Nicotine at 40 and 400 nM significantly activated constitutively active component of IK(Ach) with the maximum effect at 40 nM (an increase by 100%); similar effect was observed at -110 and -50 mV. Changes at 4 nM nicotine were negligible on average. Coapplication of 40 nM nicotine and 20 mM ethanol (which is also known to activate this current) did not show cumulative effect. In the case of acetylcholine-induced component of IK(Ach), a dual effect of nicotine and its correlation with the current magnitude in control were apparent: the current was increased by nicotine in the cells showing small current in control and vice versa. The effect of 40 and 400 nM nicotine on acetylcholine-induced component of IK(Ach) was significantly different at -110 and -50 mV. We conclude that nicotine at clinically relevant concentrations significantly increased constitutively active component of IK(Ach) and showed a dual effect on its acetylcholine-induced component, similarly as ethanol. Synchronous application of nicotine and ethanol did not cause additive effect.

Links

MUNI/A/1365/2015, interní kód MU

Name: Kardiovaskulární systém: od modelu přes terapii k prevenci (Acronym: KAMOTEPRE)

Investor: Masaryk University, Category A

Citovat

BÉBAROVÁ, Markéta, Peter MATEJOVIČ, Olga ŠVECOVÁ, Roman KULA, Milena ŠIMURDOVÁ and Jiří ŠIMURDA. Nicotine at clinically relevant concentrations affects atrial inward rectifier potassium current sensitive to acetylcholine. Naunyn-Schmiedeberg's Archives of Pharmacology. New York: Springer, 2017, vol. 390, No 5, p. 471-481. ISSN 0028-1298. Available from: https://dx.doi.org/10.1007/s00210-017-1341-z.

@article{1379558,
   author = {Bébarová, Markéta and Matejovič, Peter and Švecová, Olga and Kula, Roman and Šimurdová, Milena and Šimurda, Jiří},
   article_location = {New York},
   article_number = {5},
   doi = {http://dx.doi.org/10.1007/s00210-017-1341-z},
   keywords = {Acetylcholine-sensitive; Dual effect; Inward rectifier; Nicotine},
   language = {eng},
   issn = {0028-1298},
   journal = {Naunyn-Schmiedeberg's Archives of Pharmacology},
   title = {Nicotine at clinically relevant concentrations affects atrial inward rectifier potassium current sensitive to acetylcholine},
   volume = {390},
   year = {2017}
}

TY  - JOUR
ID  - 1379558
AU  - Bébarová, Markéta - Matejovič, Peter - Švecová, Olga - Kula, Roman - Šimurdová, Milena - Šimurda, Jiří
PY  - 2017
TI  - Nicotine at clinically relevant concentrations affects atrial inward rectifier potassium current sensitive to acetylcholine
JF  - Naunyn-Schmiedeberg's Archives of Pharmacology
VL  - 390
IS  - 5
SP  - 471-481
EP  - 471-481
PB  - Springer
SN  - 00281298
KW  - Acetylcholine-sensitive
KW  - Dual effect
KW  - Inward rectifier
KW  - Nicotine
N2  - Nicotine abuse is associated with variety of diseases including arrhythmias, most often atrial fibrillation (AF). Altered inward rectifier potassium currents including acetylcholine-sensitive current IK(Ach) are known to be related to AF pathogenesis. Since relevant data are missing, we aimed to investigate IK(Ach) changes at clinically relevant concentrations of nicotine. Experiments were performed by the whole cell patch clamp technique at 23 +/- 1 °C on isolated rat atrial myocytes. Nicotine was applied at following concentrations: 4, 40 and 400 nM; ethanol at 20 mM (0.09%). Nicotine at 40 and 400 nM significantly activated constitutively active component of IK(Ach) with the maximum effect at 40 nM (an increase by 100%); similar effect was observed at -110 and -50 mV. Changes at 4 nM nicotine were negligible on average. Coapplication of 40 nM nicotine and 20 mM ethanol (which is also known to activate this current) did not show cumulative effect. In the case of acetylcholine-induced component of IK(Ach), a dual effect of nicotine and its correlation with the current magnitude in control were apparent: the current was increased by nicotine in the cells showing small current in control and vice versa. The effect of 40 and 400 nM nicotine on acetylcholine-induced component of IK(Ach) was significantly different at -110 and -50 mV. We conclude that nicotine at clinically relevant concentrations significantly increased constitutively active component of IK(Ach) and showed a dual effect on its acetylcholine-induced component, similarly as ethanol. Synchronous application of nicotine and ethanol did not cause additive effect.
ER  -

BÉBAROVÁ, Markéta, Peter MATEJOVIČ, Olga ŠVECOVÁ, Roman KULA, Milena ŠIMURDOVÁ and Jiří ŠIMURDA. Nicotine at clinically relevant concentrations affects atrial inward rectifier potassium current sensitive to acetylcholine. \textit{Naunyn-Schmiedeberg's Archives of Pharmacology}. New York: Springer, 2017, vol.~390, No~5, p.~471-481. ISSN~0028-1298. Available from: https://dx.doi.org/10.1007/s00210-017-1341-z.

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