Detailed Information on Publication Record
2017
Nicotine at clinically relevant concentrations affects atrial inward rectifier potassium current sensitive to acetylcholine
BÉBAROVÁ, Markéta, Peter MATEJOVIČ, Olga ŠVECOVÁ, Roman KULA, Milena ŠIMURDOVÁ et. al.Basic information
Original name
Nicotine at clinically relevant concentrations affects atrial inward rectifier potassium current sensitive to acetylcholine
Authors
BÉBAROVÁ, Markéta (203 Czech Republic, guarantor, belonging to the institution), Peter MATEJOVIČ (203 Czech Republic, belonging to the institution), Olga ŠVECOVÁ (643 Russian Federation, belonging to the institution), Roman KULA (203 Czech Republic, belonging to the institution), Milena ŠIMURDOVÁ (203 Czech Republic, belonging to the institution) and Jiří ŠIMURDA (203 Czech Republic, belonging to the institution)
Edition
Naunyn-Schmiedeberg's Archives of Pharmacology, New York, Springer, 2017, 0028-1298
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
30104 Pharmacology and pharmacy
Country of publisher
United States of America
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 2.238
RIV identification code
RIV/00216224:14110/17:00096591
Organization unit
Faculty of Medicine
UT WoS
000399170600003
Keywords in English
Acetylcholine-sensitive; Dual effect; Inward rectifier; Nicotine
Tags
Tags
International impact, Reviewed
Změněno: 20/3/2018 12:07, Soňa Böhmová
Abstract
V originále
Nicotine abuse is associated with variety of diseases including arrhythmias, most often atrial fibrillation (AF). Altered inward rectifier potassium currents including acetylcholine-sensitive current IK(Ach) are known to be related to AF pathogenesis. Since relevant data are missing, we aimed to investigate IK(Ach) changes at clinically relevant concentrations of nicotine. Experiments were performed by the whole cell patch clamp technique at 23 +/- 1 °C on isolated rat atrial myocytes. Nicotine was applied at following concentrations: 4, 40 and 400 nM; ethanol at 20 mM (0.09%). Nicotine at 40 and 400 nM significantly activated constitutively active component of IK(Ach) with the maximum effect at 40 nM (an increase by 100%); similar effect was observed at -110 and -50 mV. Changes at 4 nM nicotine were negligible on average. Coapplication of 40 nM nicotine and 20 mM ethanol (which is also known to activate this current) did not show cumulative effect. In the case of acetylcholine-induced component of IK(Ach), a dual effect of nicotine and its correlation with the current magnitude in control were apparent: the current was increased by nicotine in the cells showing small current in control and vice versa. The effect of 40 and 400 nM nicotine on acetylcholine-induced component of IK(Ach) was significantly different at -110 and -50 mV. We conclude that nicotine at clinically relevant concentrations significantly increased constitutively active component of IK(Ach) and showed a dual effect on its acetylcholine-induced component, similarly as ethanol. Synchronous application of nicotine and ethanol did not cause additive effect.
Links
| MUNI/A/1365/2015, interní kód MU |
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