The level and distribution pattern of HP1 beta in the embryonic
brain correspond to those of H3K9me1/me2 but not of H3K9me3

J 2016

The level and distribution pattern of HP1 beta in the embryonic brain correspond to those of H3K9me1/me2 but not of H3K9me3

BARTOVA, Eva, Josef VEČEŘA, Jana KREJCI, Soňa LEGARTOVÁ, Jiří PACHERNÍK et. al.

Basic information

Original name

The level and distribution pattern of HP1 beta in the embryonic brain correspond to those of H3K9me1/me2 but not of H3K9me3

Authors

BARTOVA, Eva (203 Czech Republic), Josef VEČEŘA (203 Czech Republic, belonging to the institution), Jana KREJCI (203 Czech Republic), Soňa LEGARTOVÁ (703 Slovakia), Jiří PACHERNÍK (203 Czech Republic, belonging to the institution) and Stanislav KOZUBEK (203 Czech Republic)

Edition

Histochemistry and Cell Biology, Heidelberg, Springer, 2016, 0948-6143

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

Genetics and molecular biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 2.553

RIV identification code

RIV/00216224:14310/16:00094248

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1007/s00418-015-1402-7

UT WoS

000372608900009

Keywords in English

Brain sections; Epigenetics; Histones; Histone methylation; Hippocampus; Olfactory bulb

Abstract

V originále

We studied the histone signature of embryonic and adult brains to strengthen existing evidence of the importance of the histone code in mouse brain development. We analyzed the levels and distribution patterns of H3K9me1, H3K9me2, H3K9me3, and HP1 beta in both embryonic and adult brains. Western blotting showed that during mouse brain development, the levels of H3K9me1, H3K9me2, and HP1 beta exhibited almost identical trends, with the highest protein levels occurring at E15 stage. These trends differed from the relatively stable level of H3K9me3 at developmental stages E8, E13, E15, and E18. Compared with embryonic brains, adult brains were characterized by very low levels of H3K9me1/me2/me3 and HP1 beta. Manipulation of the embryonic epigenome through histone deacetylase inhibitor treatment did not affect the distribution patterns of the studied histone markers in embryonic ventricular ependyma. Similarly, Hdac3 depletion in adult animals had no effect on histone methylation in the adult hippocampus. Our results indicate that the distribution of HP1 beta in the embryonic mouse brain is related to that of H3K9me1/me2 but not to that of H3K9me3. The unique status of H3K9me3 in the brain was confirmed by its pronounced accumulation in the granular layer of the adult olfactory bulb. Moreover, among the studied proteins, H3K9me3 was the only posttranslational histone modification that was highly abundant at clusters of centromeric heterochromatin, called chromocenters. When we focused on the hippocampus, we found this region to be rich in H3K9me1 and H3K9me3, whereas H3K9me2 and HP1 beta were present at a very low level or even absent in the hippocampal blade. Taken together, these results revealed differences in the epigenome of the embryonic and adult mouse brain and showed that the adult hippocampus, the granular layer of the adult olfactory bulb, and the ventricular ependyma of the embryonic brain are colonized by specific epigenetic marks.

Links

EE2.3.30.0009, research and development project

Name: Zaměstnáním čerstvých absolventů doktorského studia k vědecké excelenci

GJ15-13443Y, research and development project

Name: Úloha hypoxií indukovaného faktoru 1 alfa ve vývoji populace neurálních kmenových buněk myši

Investor: Czech Science Foundation

Citovat

BARTOVA, Eva, Josef VEČEŘA, Jana KREJCI, Soňa LEGARTOVÁ, Jiří PACHERNÍK and Stanislav KOZUBEK. The level and distribution pattern of HP1 beta in the embryonic brain correspond to those of H3K9me1/me2 but not of H3K9me3. Histochemistry and Cell Biology. Heidelberg: Springer, 2016, vol. 145, No 4, p. 447-461. ISSN 0948-6143. Available from: https://dx.doi.org/10.1007/s00418-015-1402-7.

@article{1380311,
   author = {Bartova, Eva and Večeřa, Josef and Krejci, Jana and Legartová, Soňa and Pacherník, Jiří and Kozubek, Stanislav},
   article_location = {Heidelberg},
   article_number = {4},
   doi = {http://dx.doi.org/10.1007/s00418-015-1402-7},
   keywords = {Brain sections; Epigenetics; Histones; Histone methylation; Hippocampus; Olfactory bulb},
   language = {eng},
   issn = {0948-6143},
   journal = {Histochemistry and Cell Biology},
   title = {The level and distribution pattern of HP1 beta in the embryonic brain correspond to those of H3K9me1/me2 but not of H3K9me3},
   volume = {145},
   year = {2016}
}

TY  - JOUR
ID  - 1380311
AU  - Bartova, Eva - Večeřa, Josef - Krejci, Jana - Legartová, Soňa - Pacherník, Jiří - Kozubek, Stanislav
PY  - 2016
TI  - The level and distribution pattern of HP1 beta in the embryonic brain correspond to those of H3K9me1/me2 but not of H3K9me3
JF  - Histochemistry and Cell Biology
VL  - 145
IS  - 4
SP  - 447-461
EP  - 447-461
PB  - Springer
SN  - 09486143
KW  - Brain sections
KW  - Epigenetics
KW  - Histones
KW  - Histone methylation
KW  - Hippocampus
KW  - Olfactory bulb
N2  - We studied the histone signature of embryonic and adult brains to strengthen existing evidence of the importance of the histone code in mouse brain development. We analyzed the levels and distribution patterns of H3K9me1, H3K9me2, H3K9me3, and HP1 beta in both embryonic and adult brains. Western blotting showed that during mouse brain development, the levels of H3K9me1, H3K9me2, and HP1 beta exhibited almost identical trends, with the highest protein levels occurring at E15 stage. These trends differed from the relatively stable level of H3K9me3 at developmental stages E8, E13, E15, and E18. Compared with embryonic brains, adult brains were characterized by very low levels of H3K9me1/me2/me3 and HP1 beta. Manipulation of the embryonic epigenome through histone deacetylase inhibitor treatment did not affect the distribution patterns of the studied histone markers in embryonic ventricular ependyma. Similarly, Hdac3 depletion in adult animals had no effect on histone methylation in the adult hippocampus. Our results indicate that the distribution of HP1 beta in the embryonic mouse brain is related to that of H3K9me1/me2 but not to that of H3K9me3. The unique status of H3K9me3 in the brain was confirmed by its pronounced accumulation in the granular layer of the adult olfactory bulb. Moreover, among the studied proteins, H3K9me3 was the only posttranslational histone modification that was highly abundant at clusters of centromeric heterochromatin, called chromocenters. When we focused on the hippocampus, we found this region to be rich in H3K9me1 and H3K9me3, whereas H3K9me2 and HP1 beta were present at a very low level or even absent in the hippocampal blade. Taken together, these results revealed differences in the epigenome of the embryonic and adult mouse brain and showed that the adult hippocampus, the granular layer of the adult olfactory bulb, and the ventricular ependyma of the embryonic brain are colonized by specific epigenetic marks.
ER  -

BARTOVA, Eva, Josef VEČEŘA, Jana KREJCI, Soňa LEGARTOVÁ, Jiří PACHERNÍK and Stanislav KOZUBEK. The level and distribution pattern of HP1 beta in the embryonic brain correspond to those of H3K9me1/me2 but not of H3K9me3. \textit{Histochemistry and Cell Biology}. Heidelberg: Springer, 2016, vol.~145, No~4, p.~447-461. ISSN~0948-6143. Available from: https://dx.doi.org/10.1007/s00418-015-1402-7.

Detailed Information on Publication Record