J 2016

ADSORPCE HEPTAMERŮ S RŮZNOU STŘEDOVOU SEKVENCÍ NUKLEOTIDŮ NA RTUŤOVÉ ELEKTRODĚ

TŘÍSKOVÁ, Iveta and Libuše TRNKOVÁ

Basic information

Original name

ADSORPCE HEPTAMERŮ S RŮZNOU STŘEDOVOU SEKVENCÍ NUKLEOTIDŮ NA RTUŤOVÉ ELEKTRODĚ

Name in Czech

ADSORPCE HEPTAMERŮ S RŮZNOU STŘEDOVOU SEKVENCÍ NUKLEOTIDŮ NA RTUŤOVÉ ELEKTRODĚ

Name (in English)

The adsorption of heptamers with different central nucleotide sequence at the mercury electrode

Authors

TŘÍSKOVÁ, Iveta (203 Czech Republic) and Libuše TRNKOVÁ (203 Czech Republic, belonging to the institution)

Edition

CHEMICKÉ LISTY, Praha, Česká společnost chemická, 2016, 0009-2770

Other information

Language

Czech

Type of outcome

Článek v odborném periodiku

Field of Study

10403 Physical chemistry

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 0.387

RIV identification code

RIV/00216224:14310/16:00094256

Organization unit

Faculty of Science

UT WoS

000376183000013

Keywords (in Czech)

DNA heptamer; d(GCGAAGC); vlásenka; doba akumulace; adsorpční vrstva; voltametrie se stejnosměrným polarizačním napětí;, eliminační voltametrická procedura

Keywords in English

ELIMINATION VOLTAMMETRY; CIRCULAR-DICHROISM; D(GCGAAGC) HAIRPIN; DNA; QUADRUPLEXES; REPLICATION; PROTEIN; DISEASE; HEAT

Tags

Změněno: 23/4/2020 17:33, Mgr. Marie Šípková, DiS.

Abstract

ORIG EN

V originále

Za nejkratší a termodynamicky nejstabilnější vlásenkovou strukturu je považován DNA heptamer se sekvencí d(GCGAAGC) (cit.)6,9,10, který byl nalezen v replikačních počátcích fága X174 (cit.11), herpes simplex viru12, v promotorových oblastech heat-shock genu bakterie E. coli13 a v rRNA genech9. Struktura uvedeného heptameru byla nejdříve studována metodami NMR spektroskopie a molekulové dynamiky6. Díky chiroptické aktivitě DNA molekul byly studovány konformační vlastnosti heptamerů spektroskopií cirkulárního dichroismu (CD spektroskopií). Také UV absorpční spektroskopie byla doplněna polyakrylamidovou gelovou elektroforézou (PAGE)1,2,14. Na spektrální výzkum d(GCGAAGC) navázal elektrochemický výzkum a ten ukázal, že i cyklická voltametrie (CV) v kombinaci s eliminační voltametrickou procedurou (EVP) může přispět ke studiu struktury DNA fragmentů.

In English

The DNA fragment d(GCGAAGC), occurring in the initial replication stage of the phage.X174, in herpes simplex virus, in the promoter region of the heat-shock gene of E. coli and in rRNA genes is still in the focus of biological and biochemical research. This shortest hairpin guided us to study the relationship between the structure and function of other heptamers with changing triplet sequence in the center of the ODN molecule (AAA, GGG, CCC). Replacement of the central part of ODN was chosen because it was initially expected that the double peak of the G oxidation signal is due to the difference in the positions of G in the stem and a loop of the initially investigated d (GCGAAGC) fragment. The experiments, however, did not confirm this assumption. In this work, we have focused on the study of the interaction of selected heptamers with the surface of the mercury in order to answer the question to what extent the A + C reduction peaks and double-peak G oxidation signals are able to reflect the structures of heptamers affected by different central trinucleotide sequences. Guanine residues d(GCGGGGC) in the structure of the quadruplex are resistant to structural changes and the voltammetric responses are therefore minimized. The interactions of quadruplexes with the surface of the electrode are also weaker than interactions of hairpin structures d(GCGAAGC), d(GCAAAGC) and the duplex structure d(GCCCCGC). The presented research has shown that a small change in the central sequence not only can alter the structure (tetramolecular quadruplex, hairpin, bimolecular duplex) but also influences the electrochemical and adsorption properties of these molecules on the charged interface. Although electrochemistry is able to detect the various structural layout of the studied heptamers, it is desirable to support the electrochemical conclusions with other methods, e.g., UV-VIS absorption spectra reflecting chiroptic properties of ODNs and separation methods, such as gel electrophoresis (PAGE).