Assessment of CdS quantum dots effect on UV damage to DNA using a DNA/quantum dots structured electrochemical biosensor and DNA biosensing in solution

SVITKOVA, Veronika, Jana BLASKOVICOVA, Monika TEKELOVA, Brigitta Margit KALLAI, Teodora IGNAT, Veronika HORÁČKOVÁ, Petr SKLÁDAL, Pavel KOPEL, Vojtěch ADAM, Dana FARKASOVA and Ján LABUDA. Assessment of CdS quantum dots effect on UV damage to DNA using a DNA/quantum dots structured electrochemical biosensor and DNA biosensing in solution. Sensors and Actuators B: Chemical. Lausanne: Elsevier, 2017, vol. 243, May, p. 435-444. ISSN 0925-4005. Available from: https://dx.doi.org/10.1016/j.snb.2016.11.160.

Basic information

• Original name: Assessment of CdS quantum dots effect on UV damage to DNA using a DNA/quantum dots structured electrochemical biosensor and DNA biosensing in solution
• Authors: SVITKOVA, Veronika (703 Slovakia), Jana BLASKOVICOVA (703 Slovakia), Monika TEKELOVA (703 Slovakia), Brigitta Margit KALLAI (703 Slovakia), Teodora IGNAT (703 Slovakia), Veronika HORÁČKOVÁ (203 Czech Republic, belonging to the institution), Petr SKLÁDAL (203 Czech Republic, guarantor, belonging to the institution), Pavel KOPEL (203 Czech Republic), Vojtěch ADAM (203 Czech Republic), Dana FARKASOVA (703 Slovakia) and Ján LABUDA (703 Slovakia).
• Edition: Sensors and Actuators B: Chemical, Lausanne, Elsevier, 2017, 0925-4005.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10600 1.6 Biological sciences
• Country of publisher: Switzerland
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• Impact factor: Impact factor: 5.667
• RIV identification code: RIV/00216224:14740/17:00096688
• Organization unit: Central European Institute of Technology
• Doi: http://dx.doi.org/10.1016/j.snb.2016.11.160
• UT WoS: 000395969100055
• Keywords in English: Nanoparticles toxicity; Quantum dots; UV-C radiation; DNA damage; Biosensor; Biosensing
• Tags: rivok
• Tags: International impact, Reviewed

Abstract

Oxidation stress induced by nanoparticles is considered to be the main mechanism of their toxicity. An evaluation of oxidative damage to DNA, one of the mostly endangered molecules, belongs to general approaches used in nanomaterials cytotoxicology. However, for spherical, biochemical and other reasons, they should be differences in nanoparticles behaviour using tests based on cells and pure nucleic acid substances. The goal of the work is to detect structural damage to isolated salmon sperm double stranded DNA at its exposure to UV-C radiation (254 nm) in the presence of thiol-capped CdS quantum dots of various size and fluorescence intensity. An electrochemical DNA-based biosensor composed of a glassy carbon electrode with a layered structure of chemical modifiers dsDNA and CdS (Ps was prepared and used for the voltammetric and impedimetric evaluation of a DNA portion survived after the irradiation. The cyclic voltammetric response of the redox indicator system hexacyanoferrate(III/II) and square wave voltammetric response of the guanine and adenine moieties were measured as analytical signals. The results were verified by experiments with irradiation of DNA in the solution phase followed by voltammetric measurement of immobilized survived portion of DNA (biosensing) and by conventional agarose gel electrophoresis. Experiments showed significant effect of the fluorescent active quantum dots on the degree of DNA degradation. (C) 2016 Published by Elsevier B.V.

Links

• LQ1601, research and development project: Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR