Maleic anhydride and acetylene plasma co-polymers for the
development of effective QCM and SPR immunosensors

a 2017

Maleic anhydride and acetylene plasma co-polymers for the development of effective QCM and SPR immunosensors

MAKHNEVA, Ekaterina, Zdeněk FARKA, Adam OBRUSNÍK, Miroslav MICHLÍČEK, Petr SKLÁDAL et. al.

Basic information

Original name

Maleic anhydride and acetylene plasma co-polymers for the development of effective QCM and SPR immunosensors

Authors

MAKHNEVA, Ekaterina, Zdeněk FARKA, Adam OBRUSNÍK, Miroslav MICHLÍČEK, Petr SKLÁDAL and Lenka ZAJÍČKOVÁ

Edition

CEITEC PhD Retreat II, 2017

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

10305 Fluids and plasma physics

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Organization unit

Central European Institute of Technology

ISBN

978-80-210-8550-3

Abstract

V originále

Biosensors have been extensively developed and applied for biomedical and environmental studies. Quartz crystal microbalance (QCM) and surface plasmon resonance (SPR) biosensors appeared to be very promising tools regarding the sensitive determination of various antigens and bacteria. For the development of effective biosensors, the immobilization of the biorecognition biomolecules onto the sensor surface is always required. Carboxyl-rich films are of high interest for bio-applications thanks to their high reactivity allowing the formation of the covalent linkages between biomolecules and a surface. For most applications, a sufficiently high surface concentration of functional groups as well as the layer stability in different buffers is required. In our work, stable carboxyl-rich plasma polymers (COOH PPs) were deposited using plasma co-polymerization of maleic anhydride and acetylene at atmospheric pressure using dielectric barrier discharge (DBD). Concentrations of carboxyl groups were determined by well-known derivatization with trifluoroethanol (TFE). For the immunosensors development 20 nm thick COOH PPs were deposited onto the gold surfaces of QCM electrodes and SPR chips. Then the monoclonal antibody AL-01, specific to human serum albumin (HSA) was immobilized using EDC/NHS activation of COOH groups in the polymer. Performance of QCM and SPR immunosensors was evaluated by the immunoassay flow test.

Links

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

MAKHNEVA, Ekaterina, Zdeněk FARKA, Adam OBRUSNÍK, Miroslav MICHLÍČEK, Petr SKLÁDAL and Lenka ZAJÍČKOVÁ. Maleic anhydride and acetylene plasma co-polymers for the development of effective QCM and SPR immunosensors. In CEITEC PhD Retreat II. 2017. ISBN 978-80-210-8550-3.

@proceedings{1380718,
   author = {Makhneva, Ekaterina and Farka, Zdeněk and Obrusník, Adam and Michlíček, Miroslav and Skládal, Petr and Zajíčková, Lenka},
   booktitle = {CEITEC PhD Retreat II},
   language = {eng},
   isbn = {978-80-210-8550-3},
   title = {Maleic anhydride and acetylene plasma co-polymers for the development of effective QCM and SPR immunosensors},
   url = {https://www.ceitec.eu/ceitec-phd-retreat-ii-book-of-abstracts/f34779#page=93},
   year = {2017}
}

TY  - CONF
ID  - 1380718
AU  - Makhneva, Ekaterina - Farka, Zdeněk - Obrusník, Adam - Michlíček, Miroslav - Skládal, Petr - Zajíčková, Lenka
PY  - 2017
TI  - Maleic anhydride and acetylene plasma co-polymers for the development of effective QCM and SPR immunosensors
SN  - 9788021085503
UR  - https://www.ceitec.eu/ceitec-phd-retreat-ii-book-of-abstracts/f34779#page=93
N2  - Biosensors have been extensively developed and applied for biomedical and environmental studies. Quartz crystal microbalance (QCM) and surface plasmon resonance (SPR) biosensors appeared to be very promising tools regarding the sensitive determination of various antigens and bacteria. For the development of effective biosensors, the immobilization of the biorecognition biomolecules onto the sensor surface is always required. Carboxyl-rich films are of high interest for bio-applications thanks to their high reactivity allowing the formation of the covalent linkages between biomolecules and a surface. For most applications, a sufficiently high surface concentration of functional groups as well as the layer stability in different buffers is required. In our work, stable carboxyl-rich plasma polymers (COOH PPs) were deposited using plasma co-polymerization of maleic anhydride and acetylene at atmospheric pressure using dielectric barrier discharge (DBD). Concentrations of carboxyl groups were determined by well-known derivatization with trifluoroethanol (TFE). For the immunosensors development 20 nm thick COOH PPs were deposited onto the gold surfaces of QCM electrodes and SPR chips. Then the monoclonal antibody AL-01, specific to human serum albumin (HSA) was immobilized using EDC/NHS activation of COOH groups in the polymer. Performance of QCM and SPR immunosensors was evaluated by the immunoassay flow test.
ER  -

MAKHNEVA, Ekaterina, Zdeněk FARKA, Adam OBRUSNÍK, Miroslav MICHLÍČEK, Petr SKLÁDAL and Lenka ZAJÍČKOVÁ. Maleic anhydride and acetylene plasma co-polymers for the development of effective QCM and SPR immunosensors. In \textit{CEITEC PhD Retreat II}. 2017. ISBN~978-80-210-8550-3.

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