ULTRASTRUCTURAL OBSERVATIONS ON REPRESENTATIVES FROM FAMILY
DIPLOZOIDAE (MONOGENEA)

a 2017

ULTRASTRUCTURAL OBSERVATIONS ON REPRESENTATIVES FROM FAMILY DIPLOZOIDAE (MONOGENEA)

KONSTANZOVÁ, Veronika, Božena KOUBKOVÁ and Milan GELNAR

Basic information

Original name

ULTRASTRUCTURAL OBSERVATIONS ON REPRESENTATIVES FROM FAMILY DIPLOZOIDAE (MONOGENEA)

Authors

KONSTANZOVÁ, Veronika (203 Czech Republic, belonging to the institution), Božena KOUBKOVÁ (203 Czech Republic, belonging to the institution) and Milan GELNAR (203 Czech Republic, guarantor, belonging to the institution)

Edition

23rd Helminthological Days, Duchonka - Pension Slniečko, Slovakia, 2017

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

10600 1.6 Biological sciences

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/17:00094749

Organization unit

Faculty of Science

ISBN

978-80-968473-8-9

Keywords in English

diplozoon; monogenea; ultrastructure

Abstract

V originále

Diplozoons are blood-feeding ectoparasites with specific life-cycle, parasitizing mainly on gills of cyprinid fish, where they can cause mechanical damage to the gill filaments, initiating the development of secondary infections (bacterial, mycotic) and anemia. During the diplozoon life cycle two larvae, the diporpae, pair and subsequently fuse permanently producing the typical X-shaped diplozoon body arrangement, this is a unique character for Diplozoidae. Although these worms have been the subject of numerous taxonomical, phylogenetic, and ecological studies, the detailed studies of their inner systems have remained relatively neglected. This presentation is based in comprehensive study focusing on diplozoids morphology and ultrastructure, looking for phylogenetic and functional indicators of their specific life strategy. The samples of several diplozoon species were collected from the gills of different species of host fish D. paradoxum (A. brama), E. nipponicum (C. carpio), P. homoion (A. alburnus) and P. bliccae (B. bjoerkna), which were caught in the littoral zone of the Mušov lowland reservoir. The fish gills were extracted and checked for the presence of all diplozoid ontogenetic stages. Parasites intended for transmission electron microscopy (TEM) were washed in freshwater to remove any remaining mucus and then fixed in 2% osmium tetroxide and dehydrated through an ascending acetone series. The dehydrated samples were embedded in Spurr resin. In order to obtain a better orientation in basic diplozoon morphology several semi-thin sections (0.5 µm) of whole worm bodies were cut using a Leica EM UC6i. Sections were stained with toluidine blue. Ultrathin sections were contrasted with uranyl acetate, followed by citrate, and examined using a JEOL JEM-1010 TEM. The presentation focusses on the morphological and ultrastructural features of different body structures such as neodermal surface, gastrointestinal tract, excretory system and attachment clamps.

Links

GBP505/12/G112, research and development project

Name: ECIP - Evropské centrum ichtyoparazitologie

Investor: Czech Science Foundation

MSM0021622416, plan (intention)

Name: Diverzita biotických společenstev a populací: kauzální analýza variability v prostoru a čase

Investor: Ministry of Education, Youth and Sports of the CR, Diversity of Biotic Communities and Populations: Causal Analysis of variation in space and time

Citovat

KONSTANZOVÁ, Veronika, Božena KOUBKOVÁ and Milan GELNAR. ULTRASTRUCTURAL OBSERVATIONS ON REPRESENTATIVES FROM FAMILY DIPLOZOIDAE (MONOGENEA). In 23rd Helminthological Days, Duchonka - Pension Slniečko, Slovakia. 2017. ISBN 978-80-968473-8-9.

@proceedings{1380772,
   author = {Konstanzová, Veronika and Koubková, Božena and Gelnar, Milan},
   booktitle = {23rd Helminthological Days, Duchonka - Pension Slniečko, Slovakia},
   keywords = {diplozoon; monogenea; ultrastructure},
   language = {eng},
   isbn = {978-80-968473-8-9},
   title = {ULTRASTRUCTURAL OBSERVATIONS ON REPRESENTATIVES FROM FAMILY DIPLOZOIDAE (MONOGENEA)},
   year = {2017}
}

TY  - CONF
ID  - 1380772
AU  - Konstanzová, Veronika - Koubková, Božena - Gelnar, Milan
PY  - 2017
TI  - ULTRASTRUCTURAL OBSERVATIONS ON REPRESENTATIVES FROM FAMILY DIPLOZOIDAE (MONOGENEA)
SN  - 9788096847389
KW  - diplozoon
KW  - monogenea
KW  - ultrastructure
N2  - Diplozoons are blood-feeding ectoparasites with specific life-cycle, parasitizing mainly on gills of cyprinid fish, where they can cause mechanical damage to the gill filaments, initiating the development of secondary infections (bacterial, mycotic) and anemia. During the diplozoon life cycle two larvae, the diporpae, pair and subsequently fuse permanently producing the typical X-shaped diplozoon body arrangement, this is a unique character for Diplozoidae. Although these worms have been the subject of numerous taxonomical, phylogenetic, and ecological studies, the detailed studies of their inner systems have remained relatively neglected. This presentation is based in comprehensive study focusing on diplozoids morphology and ultrastructure, looking for phylogenetic and functional indicators of their specific life strategy. The samples of several diplozoon species were collected from the gills of different species of host fish D. paradoxum (A. brama), E. nipponicum (C. carpio), P. homoion (A. alburnus) and P. bliccae (B. bjoerkna), which were caught in the littoral zone of the Mušov lowland reservoir. The fish gills were extracted and checked for the presence of all diplozoid ontogenetic stages. Parasites intended for transmission electron microscopy (TEM) were washed in freshwater to remove any remaining mucus and then fixed in 2% osmium tetroxide and dehydrated through an ascending acetone series. The dehydrated samples were embedded in Spurr resin. In order to obtain a better orientation in basic diplozoon morphology several semi-thin sections (0.5 µm) of whole worm bodies were cut using a Leica EM UC6i. Sections were stained with toluidine blue. Ultrathin sections were contrasted with uranyl acetate, followed by citrate, and examined using a JEOL JEM-1010 TEM. The presentation focusses on the morphological and ultrastructural features of different body structures such as neodermal surface, gastrointestinal tract, excretory system and attachment clamps.
ER  -

KONSTANZOVÁ, Veronika, Božena KOUBKOVÁ and Milan GELNAR. ULTRASTRUCTURAL OBSERVATIONS ON REPRESENTATIVES FROM FAMILY DIPLOZOIDAE (MONOGENEA). In \textit{23rd Helminthological Days, Duchonka - Pension Slniečko, Slovakia}. 2017. ISBN~978-80-968473-8-9.

Detailed Information on Publication Record