Other formats:
BibTeX
LaTeX
RIS
@misc{1380786,
author = {Pešl, Martin and Přibyl, Jan and Aćimović, Ivana and Vilotić, Aleksandra and Jelínková, Šárka and Salykin, Anton and Lacampagne, Alain and Dvořák, Petr and Meli, Albano and Skládal, Petr and Rotrekl, Vladimír},
booktitle = {Czech Society of Cardiology Annual meeting, Brno},
keywords = {Micromechanical biosensor; Human stem cell; Cardiomyocyte contraction; Drug testing},
language = {eng},
title = {Atomic force microscopy combined with human pluripotent stem cell derived cardiomyocytes for biomechanical sensing},
year = {2017}
}
TY - SLIDE
ID - 1380786
AU - Pešl, Martin - Přibyl, Jan - Aćimović, Ivana - Vilotić, Aleksandra - Jelínková, Šárka - Salykin, Anton - Lacampagne, Alain - Dvořák, Petr - Meli, Albano - Skládal, Petr - Rotrekl, Vladimír
PY - 2017
TI - Atomic force microscopy combined with human pluripotent stem cell derived cardiomyocytes for biomechanical sensing
KW - Micromechanical biosensor
KW - Human stem cell
KW - Cardiomyocyte contraction
KW - Drug testing
N2 - Cardiomyocyte contraction and relaxation are important parameters of cardiac function altered in many heart pathologies. Biosensing of these parameters represents an important tool in drug development and disease modeling. Human embryonic stem cells and especially patient specific induced pluripotent stem cell-derived cardiomyocytes are well established as cardiac disease model.. Here, a live stem cell derived embryoid body (EB) based cardiac cell syncytium served as a biorecognition element coupled to the microcantilever probe from atomic force microscope thus providing reliable micromechanical cellular biosensor suitable for whole-day testing. The biosensor was optimized regarding the type of cantilever, temperature and exchange of media; in combination with standardized protocol, it allowed testing of compounds and conditions affecting the biomechanical properties of EB. The studied effectors included calcium , drugs modulating the catecholaminergic fight-or-flight stress response such as the beta-adrenergic blocker metoprolol and the beta-adrenergic agonist isoproterenol. Arrhythmogenic effects were studied using caffeine. Furthermore, with EBs originating from patient's stem cells, this biosensor can help to characterize heart diseases such as dystrophies.
ER -
PEŠL, Martin, Jan PŘIBYL, Ivana A$\backslash$'CIMOVI$\backslash$'C, Aleksandra VILOTI$\backslash$'C, Šárka JELÍNKOVÁ, Anton SALYKIN, Alain LACAMPAGNE, Petr DVOŘÁK, Albano MELI, Petr SKLÁDAL and Vladimír ROTREKL. Atomic force microscopy combined with human pluripotent stem cell derived cardiomyocytes for biomechanical sensing. In \textit{Czech Society of Cardiology Annual meeting, Brno}. 2017.
|
