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@article{1380793,
author = {Vanerkova, M. and Malisova, B. and Kotaskova, I. and Holá, Veronika and Růžička, Filip and Freiberger, Tomáš},
article_location = {Praha},
article_number = {6},
doi = {http://dx.doi.org/10.1007/s12223-017-0526-7},
keywords = {Pseudomonas aeruginosa},
language = {eng},
issn = {0015-5632},
journal = {Folia microbiologica},
title = {Biofilm formation, antibiotic susceptibility and RAPD genotypes in Pseudomonas aeruginosa clinical strains isolated from single centre intensive care unit patients},
volume = {62},
year = {2017}
}
TY - JOUR
ID - 1380793
AU - Vanerkova, M. - Malisova, B. - Kotaskova, I. - Holá, Veronika - Růžička, Filip - Freiberger, Tomáš
PY - 2017
TI - Biofilm formation, antibiotic susceptibility and RAPD genotypes in Pseudomonas aeruginosa clinical strains isolated from single centre intensive care unit patients
JF - Folia microbiologica
VL - 62
IS - 6
SP - 531-538
EP - 531-538
PB - Mikrobiologický ústav Praha AV ČR
SN - 00155632
KW - Pseudomonas aeruginosa
N2 - The aim of this study was to analyse genotypes, antimicrobial susceptibility patterns and serotypes in Pseudomonas aeruginosa clinical strains, including the clonal dissemination of particular strains throughout various intensive care units in one medical centre. Using random amplified polymorphic DNA (RAPD-PCR) and P. aeruginosa antisera, 22 different genotypes and 8 serotypes were defined among 103 isolates from 48 patients. No direct association between P. aeruginosa strain genotypes and serotypes was observed. RAPD typing in strains with the same serotype revealed different genotypes and, on the contrary, most strains with a different serotype displayed the same amplification pattern. The resulting banding patterns showed a high degree of genetic heterogeneity among all isolates from the patients examined, suggesting a non-clonal relationship between isolates from these patients. A higher degree of antibiotic resistance and stronger biofilm production in common genotypes compared to rare ones and genetic homogeneity of the most resistant strains indicated the role of antibiotic pressure in acquiring resistant and more virulent strains in our hospital. In conclusion, genetic characterisation of P. aeruginosa strains using RAPD method was shown to be more accurate in epidemiological analyses than phenotyping.
ER -
VANERKOVA, M., B. MALISOVA, I. KOTASKOVA, Veronika HOLÁ, Filip RŮŽIČKA and Tomáš FREIBERGER. Biofilm formation, antibiotic susceptibility and RAPD genotypes in Pseudomonas aeruginosa clinical strains isolated from single centre intensive care unit patients. \textit{Folia microbiologica}. Praha: Mikrobiologický ústav Praha AV ČR, 2017, vol.~62, No~6, p.~531-538. ISSN~0015-5632. Available from: https://dx.doi.org/10.1007/s12223-017-0526-7.
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