Detailed Information on Publication Record
2017
Biofilm formation, antibiotic susceptibility and RAPD genotypes in Pseudomonas aeruginosa clinical strains isolated from single centre intensive care unit patients
VANERKOVA, M., B. MALISOVA, I. KOTASKOVA, Veronika HOLÁ, Filip RŮŽIČKA et. al.Basic information
Original name
Biofilm formation, antibiotic susceptibility and RAPD genotypes in Pseudomonas aeruginosa clinical strains isolated from single centre intensive care unit patients
Authors
VANERKOVA, M. (203 Czech Republic), B. MALISOVA (203 Czech Republic), I. KOTASKOVA (203 Czech Republic), Veronika HOLÁ (203 Czech Republic, belonging to the institution), Filip RŮŽIČKA (203 Czech Republic, belonging to the institution) and Tomáš FREIBERGER (203 Czech Republic, guarantor, belonging to the institution)
Edition
Folia microbiologica, Praha, Mikrobiologický ústav Praha AV ČR, 2017, 0015-5632
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10606 Microbiology
Country of publisher
Netherlands
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 1.311
RIV identification code
RIV/00216224:14110/17:00095637
Organization unit
Faculty of Medicine
UT WoS
000412456500011
Keywords in English
Pseudomonas aeruginosa
Tags
Tags
International impact, Reviewed
Změněno: 21/3/2018 13:31, Soňa Böhmová
Abstract
V originále
The aim of this study was to analyse genotypes, antimicrobial susceptibility patterns and serotypes in Pseudomonas aeruginosa clinical strains, including the clonal dissemination of particular strains throughout various intensive care units in one medical centre. Using random amplified polymorphic DNA (RAPD-PCR) and P. aeruginosa antisera, 22 different genotypes and 8 serotypes were defined among 103 isolates from 48 patients. No direct association between P. aeruginosa strain genotypes and serotypes was observed. RAPD typing in strains with the same serotype revealed different genotypes and, on the contrary, most strains with a different serotype displayed the same amplification pattern. The resulting banding patterns showed a high degree of genetic heterogeneity among all isolates from the patients examined, suggesting a non-clonal relationship between isolates from these patients. A higher degree of antibiotic resistance and stronger biofilm production in common genotypes compared to rare ones and genetic homogeneity of the most resistant strains indicated the role of antibiotic pressure in acquiring resistant and more virulent strains in our hospital. In conclusion, genetic characterisation of P. aeruginosa strains using RAPD method was shown to be more accurate in epidemiological analyses than phenotyping.
Links
| NV16-31593A, research and development project |
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