Human Escherichia coli isolates from hemocultures: Septicemia
linked to urogenital tract infections is caused by isolates
harboring more virulence genes than bacteraemia linked to other
conditions

J 2017

Human Escherichia coli isolates from hemocultures: Septicemia linked to urogenital tract infections is caused by isolates harboring more virulence genes than bacteraemia linked to other conditions

MICENKOVÁ, Lenka; Alžbeta BEŇOVÁ; Lucia FRANKOVIČOVÁ; Juraj BOSÁK; Martin VRBA et. al.

Základní údaje

Originální název

Human Escherichia coli isolates from hemocultures: Septicemia linked to urogenital tract infections is caused by isolates harboring more virulence genes than bacteraemia linked to other conditions

Autoři

MICENKOVÁ, Lenka; Alžbeta BEŇOVÁ; Lucia FRANKOVIČOVÁ; Juraj BOSÁK; Martin VRBA; Alena ŠEVČÍKOVÁ; Marta KMEŤOVÁ a David ŠMAJS

Vydání

International Journal of Medical Microbiology, Jena, Elsevier GmbH, 2017, 1438-4221

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10606 Microbiology

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 3.298

Kód RIV

RIV/00216224:14110/17:00094764

Organizační jednotka

Lékařská fakulta

DOI

https://doi.org/10.1016/j.ijmm.2017.02.003

UT WoS

000400218000005

EID Scopus

2-s2.0-85014142946

Klíčová slova anglicky

E. coli; Hemoculture; Blood; Virulence; Bacteriocin; Phylogenetic group

Štítky

EL OK

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 21. 3. 2018 16:52, Soňa Böhmová

Anotace

V originále

Escherichia coli is the most common cause of bloodstream infections and community-acquired sepsis. The main aim of this study was to determine virulence characteristics of E. coli isolates from hemocultures of patients with a primary disease of urogenital tract, digestive system, a neoplastic blood disease, or other conditions. Results from a set of 314 E. coli isolates from hemocultures were compared to data from a previously published analysis of 1283 fecal commensal E. coli isolates. Genetic profiling of the 314 E. coli isolates involved determination of phylogenetic group (A, B1, B2, D, C, E, and F), identification of 21 virulence factors, as well as 30 bacteriocin-encoding determinants. Pulsed-field gel electrophoresis was used to analyze clonal character of the hemoculture-derived isolates. The E. coli isolates from hemocultures belonged mainly to phylogenetic groups B2 (59.9%) and D (21.0%), and less frequently to phylogroups A (10.2%) and B1 (5.7%). Commonly detected virulence factors included adhesins (fimA 92.0%, pap 47.1%, and sfa 26.8%), and iron-uptake encoding genes (fyuA 87.9%, fepC 79.6%, aer 70.7%, iucC 68.2%, and ireA 13.7%), followed by colibactin (pits island 31.5%), and cytotoxic necrotizing factor (cnfl 11.1%). A higher frequency of microcin producers (and microcin M determinant) and a lower frequency of colicin Ib and microcin B17 was found in hemoculture-derived isolates compared to commensal fecal isolates. E. coli isolates from hemocultures harbored more virulence genes compared to fecal E. coli isolates. In addition, hemoculture E. coli isolates from patients with primary diagnosis related to urogenital tract were clearly different and more virulence genes were detected in these isolates compared to both fecal isolates and hemoculture-derived isolates from patients with blood and gastrointestinal diseases.

Návaznosti

GA16-21649S, projekt VaV

Název: Molekulární charakterizace nových bakteriocinů identifikovaných v rodech Escherichia a Shigella

Investor: Grantová agentura ČR, Molekulární charakterizace nových bakteriocinů identifikovaných v rodech Escherichia a Shigella

Citovat

MICENKOVÁ, Lenka; Alžbeta BEŇOVÁ; Lucia FRANKOVIČOVÁ; Juraj BOSÁK; Martin VRBA; Alena ŠEVČÍKOVÁ; Marta KMEŤOVÁ a David ŠMAJS. Human Escherichia coli isolates from hemocultures: Septicemia linked to urogenital tract infections is caused by isolates harboring more virulence genes than bacteraemia linked to other conditions. International Journal of Medical Microbiology. Jena: Elsevier GmbH, 2017, roč. 307, č. 3, s. 182-189. ISSN 1438-4221. Dostupné z: https://doi.org/10.1016/j.ijmm.2017.02.003.

@article{1381853,
   author = {Micenková, Lenka and Beňová, Alžbeta and Frankovičová, Lucia and Bosák, Juraj and Vrba, Martin and Ševčíková, Alena and Kmeťová, Marta and Šmajs, David},
   article_location = {Jena},
   article_number = {3},
   doi = {https://doi.org/10.1016/j.ijmm.2017.02.003},
   keywords = {E. coli; Hemoculture; Blood; Virulence; Bacteriocin; Phylogenetic group},
   language = {eng},
   issn = {1438-4221},
   journal = {International Journal of Medical Microbiology},
   title = {Human Escherichia coli isolates from hemocultures: Septicemia linked to urogenital tract infections is caused by isolates harboring more virulence genes than bacteraemia linked to other conditions},
   volume = {307},
   year = {2017}
}

TY  - JOUR
ID  - 1381853
AU  - Micenková, Lenka - Beňová, Alžbeta - Frankovičová, Lucia - Bosák, Juraj - Vrba, Martin - Ševčíková, Alena - Kmeťová, Marta - Šmajs, David
PY  - 2017
TI  - Human Escherichia coli isolates from hemocultures: Septicemia linked to urogenital tract infections is caused by isolates harboring more virulence genes than bacteraemia linked to other conditions
JF  - International Journal of Medical Microbiology
VL  - 307
IS  - 3
SP  - 182-189
EP  - 182-189
PB  - Elsevier GmbH
SN  - 14384221
KW  - E. coli
KW  - Hemoculture
KW  - Blood
KW  - Virulence
KW  - Bacteriocin
KW  - Phylogenetic group
N2  - Escherichia coli is the most common cause of bloodstream infections and community-acquired sepsis. The main aim of this study was to determine virulence characteristics of E. coli isolates from hemocultures of patients with a primary disease of urogenital tract, digestive system, a neoplastic blood disease, or other conditions. Results from a set of 314 E. coli isolates from hemocultures were compared to data from a previously published analysis of 1283 fecal commensal E. coli isolates. Genetic profiling of the 314 E. coli isolates involved determination of phylogenetic group (A, B1, B2, D, C, E, and F), identification of 21 virulence factors, as well as 30 bacteriocin-encoding determinants. Pulsed-field gel electrophoresis was used to analyze clonal character of the hemoculture-derived isolates. The E. coli isolates from hemocultures belonged mainly to phylogenetic groups B2 (59.9%) and D (21.0%), and less frequently to phylogroups A (10.2%) and B1 (5.7%). Commonly detected virulence factors included adhesins (fimA 92.0%, pap 47.1%, and sfa 26.8%), and iron-uptake encoding genes (fyuA 87.9%, fepC 79.6%, aer 70.7%, iucC 68.2%, and ireA 13.7%), followed by colibactin (pits island 31.5%), and cytotoxic necrotizing factor (cnfl 11.1%). A higher frequency of microcin producers (and microcin M determinant) and a lower frequency of colicin Ib and microcin B17 was found in hemoculture-derived isolates compared to commensal fecal isolates. E. coli isolates from hemocultures harbored more virulence genes compared to fecal E. coli isolates. In addition, hemoculture E. coli isolates from patients with primary diagnosis related to urogenital tract were clearly different and more virulence genes were detected in these isolates compared to both fecal isolates and hemoculture-derived isolates from patients with blood and gastrointestinal diseases.
ER  -

MICENKOVÁ, Lenka; Alžbeta BEŇOVÁ; Lucia FRANKOVIČOVÁ; Juraj BOSÁK; Martin VRBA; Alena ŠEVČÍKOVÁ; Marta KMEŤOVÁ a David ŠMAJS. Human Escherichia coli isolates from hemocultures: Septicemia linked to urogenital tract infections is caused by isolates harboring more virulence genes than bacteraemia linked to other conditions. \textit{International Journal of Medical Microbiology}. Jena: Elsevier GmbH, 2017, roč.~307, č.~3, s.~182-189. ISSN~1438-4221. Dostupné z: https://doi.org/10.1016/j.ijmm.2017.02.003.

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