Human Escherichia coli isolates from hemocultures: Septicemia
linked to urogenital tract infections is caused by isolates
harboring more virulence genes than bacteraemia linked to other
conditions

J 2017

Human Escherichia coli isolates from hemocultures: Septicemia linked to urogenital tract infections is caused by isolates harboring more virulence genes than bacteraemia linked to other conditions

MICENKOVÁ, Lenka, Alžbeta BEŇOVÁ, Lucia FRANKOVIČOVÁ, Juraj BOSÁK, Martin VRBA et. al.

Basic information

Original name

Human Escherichia coli isolates from hemocultures: Septicemia linked to urogenital tract infections is caused by isolates harboring more virulence genes than bacteraemia linked to other conditions

Authors

MICENKOVÁ, Lenka (703 Slovakia, belonging to the institution), Alžbeta BEŇOVÁ (703 Slovakia, belonging to the institution), Lucia FRANKOVIČOVÁ (703 Slovakia), Juraj BOSÁK (703 Slovakia, belonging to the institution), Martin VRBA (203 Czech Republic), Alena ŠEVČÍKOVÁ (203 Czech Republic), Marta KMEŤOVÁ (703 Slovakia) and David ŠMAJS (203 Czech Republic, guarantor, belonging to the institution)

Edition

International Journal of Medical Microbiology, Jena, Elsevier GmbH, 2017, 1438-4221

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10606 Microbiology

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 3.298

RIV identification code

RIV/00216224:14110/17:00094764

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1016/j.ijmm.2017.02.003

UT WoS

000400218000005

Keywords in English

E. coli; Hemoculture; Blood; Virulence; Bacteriocin; Phylogenetic group

Abstract

V originále

Escherichia coli is the most common cause of bloodstream infections and community-acquired sepsis. The main aim of this study was to determine virulence characteristics of E. coli isolates from hemocultures of patients with a primary disease of urogenital tract, digestive system, a neoplastic blood disease, or other conditions. Results from a set of 314 E. coli isolates from hemocultures were compared to data from a previously published analysis of 1283 fecal commensal E. coli isolates. Genetic profiling of the 314 E. coli isolates involved determination of phylogenetic group (A, B1, B2, D, C, E, and F), identification of 21 virulence factors, as well as 30 bacteriocin-encoding determinants. Pulsed-field gel electrophoresis was used to analyze clonal character of the hemoculture-derived isolates. The E. coli isolates from hemocultures belonged mainly to phylogenetic groups B2 (59.9%) and D (21.0%), and less frequently to phylogroups A (10.2%) and B1 (5.7%). Commonly detected virulence factors included adhesins (fimA 92.0%, pap 47.1%, and sfa 26.8%), and iron-uptake encoding genes (fyuA 87.9%, fepC 79.6%, aer 70.7%, iucC 68.2%, and ireA 13.7%), followed by colibactin (pits island 31.5%), and cytotoxic necrotizing factor (cnfl 11.1%). A higher frequency of microcin producers (and microcin M determinant) and a lower frequency of colicin Ib and microcin B17 was found in hemoculture-derived isolates compared to commensal fecal isolates. E. coli isolates from hemocultures harbored more virulence genes compared to fecal E. coli isolates. In addition, hemoculture E. coli isolates from patients with primary diagnosis related to urogenital tract were clearly different and more virulence genes were detected in these isolates compared to both fecal isolates and hemoculture-derived isolates from patients with blood and gastrointestinal diseases.

Links

GA16-21649S, research and development project

Name: Molekulární charakterizace nových bakteriocinů identifikovaných v rodech Escherichia a Shigella

Investor: Czech Science Foundation

Citovat

MICENKOVÁ, Lenka, Alžbeta BEŇOVÁ, Lucia FRANKOVIČOVÁ, Juraj BOSÁK, Martin VRBA, Alena ŠEVČÍKOVÁ, Marta KMEŤOVÁ and David ŠMAJS. Human Escherichia coli isolates from hemocultures: Septicemia linked to urogenital tract infections is caused by isolates harboring more virulence genes than bacteraemia linked to other conditions. International Journal of Medical Microbiology. Jena: Elsevier GmbH, 2017, vol. 307, No 3, p. 182-189. ISSN 1438-4221. Available from: https://dx.doi.org/10.1016/j.ijmm.2017.02.003.

@article{1381853,
   author = {Micenková, Lenka and Beňová, Alžbeta and Frankovičová, Lucia and Bosák, Juraj and Vrba, Martin and Ševčíková, Alena and Kmeťová, Marta and Šmajs, David},
   article_location = {Jena},
   article_number = {3},
   doi = {http://dx.doi.org/10.1016/j.ijmm.2017.02.003},
   keywords = {E. coli; Hemoculture; Blood; Virulence; Bacteriocin; Phylogenetic group},
   language = {eng},
   issn = {1438-4221},
   journal = {International Journal of Medical Microbiology},
   title = {Human Escherichia coli isolates from hemocultures: Septicemia linked to urogenital tract infections is caused by isolates harboring more virulence genes than bacteraemia linked to other conditions},
   volume = {307},
   year = {2017}
}

TY  - JOUR
ID  - 1381853
AU  - Micenková, Lenka - Beňová, Alžbeta - Frankovičová, Lucia - Bosák, Juraj - Vrba, Martin - Ševčíková, Alena - Kmeťová, Marta - Šmajs, David
PY  - 2017
TI  - Human Escherichia coli isolates from hemocultures: Septicemia linked to urogenital tract infections is caused by isolates harboring more virulence genes than bacteraemia linked to other conditions
JF  - International Journal of Medical Microbiology
VL  - 307
IS  - 3
SP  - 182-189
EP  - 182-189
PB  - Elsevier GmbH
SN  - 14384221
KW  - E. coli
KW  - Hemoculture
KW  - Blood
KW  - Virulence
KW  - Bacteriocin
KW  - Phylogenetic group
N2  - Escherichia coli is the most common cause of bloodstream infections and community-acquired sepsis. The main aim of this study was to determine virulence characteristics of E. coli isolates from hemocultures of patients with a primary disease of urogenital tract, digestive system, a neoplastic blood disease, or other conditions. Results from a set of 314 E. coli isolates from hemocultures were compared to data from a previously published analysis of 1283 fecal commensal E. coli isolates. Genetic profiling of the 314 E. coli isolates involved determination of phylogenetic group (A, B1, B2, D, C, E, and F), identification of 21 virulence factors, as well as 30 bacteriocin-encoding determinants. Pulsed-field gel electrophoresis was used to analyze clonal character of the hemoculture-derived isolates. The E. coli isolates from hemocultures belonged mainly to phylogenetic groups B2 (59.9%) and D (21.0%), and less frequently to phylogroups A (10.2%) and B1 (5.7%). Commonly detected virulence factors included adhesins (fimA 92.0%, pap 47.1%, and sfa 26.8%), and iron-uptake encoding genes (fyuA 87.9%, fepC 79.6%, aer 70.7%, iucC 68.2%, and ireA 13.7%), followed by colibactin (pits island 31.5%), and cytotoxic necrotizing factor (cnfl 11.1%). A higher frequency of microcin producers (and microcin M determinant) and a lower frequency of colicin Ib and microcin B17 was found in hemoculture-derived isolates compared to commensal fecal isolates. E. coli isolates from hemocultures harbored more virulence genes compared to fecal E. coli isolates. In addition, hemoculture E. coli isolates from patients with primary diagnosis related to urogenital tract were clearly different and more virulence genes were detected in these isolates compared to both fecal isolates and hemoculture-derived isolates from patients with blood and gastrointestinal diseases.
ER  -

MICENKOVÁ, Lenka, Alžbeta BEŇOVÁ, Lucia FRANKOVIČOVÁ, Juraj BOSÁK, Martin VRBA, Alena ŠEVČÍKOVÁ, Marta KMEŤOVÁ and David ŠMAJS. Human Escherichia coli isolates from hemocultures: Septicemia linked to urogenital tract infections is caused by isolates harboring more virulence genes than bacteraemia linked to other conditions. \textit{International Journal of Medical Microbiology}. Jena: Elsevier GmbH, 2017, vol.~307, No~3, p.~182-189. ISSN~1438-4221. Available from: https://dx.doi.org/10.1016/j.ijmm.2017.02.003.

Detailed Information on Publication Record