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@article{1381950,
author = {Spurný, Radovan and Pridal, Antonin and Pálková, Lenka and Tran Kiem, Hoa Khanh and de Miranda, Joachim R. and Plevka, Pavel},
article_location = {WASHINGTON},
article_number = {6},
doi = {http://dx.doi.org/10.1128/JVI.02100-16},
keywords = {virus; Apis mellifera; honey bee; honeybee; Picornavirales; Dicistroviridae; Cripavirus; Triatovirus; virion; structure; X ray; crystallography; capsid; insect disease; X-ray crystallography},
language = {eng},
issn = {0022-538X},
journal = {JOURNAL OF VIROLOGY},
title = {Virion Structure of Black Queen Cell Virus, a Common Honeybee Pathogen},
url = {http://jvi.asm.org/content/91/6/e02100-16},
volume = {91},
year = {2017}
}
TY - JOUR
ID - 1381950
AU - Spurný, Radovan - Pridal, Antonin - Pálková, Lenka - Tran Kiem, Hoa Khanh - de Miranda, Joachim R. - Plevka, Pavel
PY - 2017
TI - Virion Structure of Black Queen Cell Virus, a Common Honeybee Pathogen
JF - JOURNAL OF VIROLOGY
VL - 91
IS - 6
SP - nestránkováno
EP - nestránkováno
PB - AMER SOC MICROBIOLOGY
SN - 0022538X
KW - virus
KW - Apis mellifera
KW - honey bee
KW - honeybee
KW - Picornavirales
KW - Dicistroviridae
KW - Cripavirus
KW - Triatovirus
KW - virion
KW - structure
KW - X ray
KW - crystallography
KW - capsid
KW - insect disease
KW - X-ray crystallography
UR - http://jvi.asm.org/content/91/6/e02100-16
L2 - http://jvi.asm.org/content/91/6/e02100-16
N2 - Viral diseases are a major threat to honeybee (Apis mellifera) populations worldwide and therefore an important factor in reliable crop pollination and food security. Black queen cell virus (BQCV) is the etiological agent of a fatal disease of honeybee queen larvae and pupae. The virus belongs to the genus Triatovirus from the family Dicistroviridae, which is part of the order Picornavirales. Here we present a crystal structure of BQCV determined to a resolution of 3.4 angstrom. The virion is formed by 60 copies of each of the major capsid proteins VP1, VP2, and VP3; however, there is no density corresponding to a 75-residue-long minor capsid protein VP4 encoded by the BQCV genome. We show that the VP4 subunits are present in the crystallized virions that are infectious. This aspect of the BQCV virion is similar to that of the previously characterized triatoma virus and supports the recent establishment of the separate genus Triatovirus within the family Dicistroviridae. The C terminus of VP1 and CD loops of capsid proteins VP1 and VP3 of BQCV form 34-angstrom-tall finger-like protrusions at the virion surface. The protrusions are larger than those of related dicistroviruses. IMPORTANCE The western honeybee is the most important pollinator of all, and it is required to sustain the agricultural production and biodiversity of wild flowering plants. However, honeybee populations worldwide are suffering from virus infections that cause colony losses. One of the most common, and least known, honeybee pathogens is black queen cell virus (BQCV), which at high titers causes queen larvae and pupae to turn black and die. Here we present the three-dimensional virion structure of BQCV, determined by X-ray crystallography. The structure of BQCV reveals large protrusions on the virion surface. Capsid protein VP1 of BQCV does not contain a hydrophobic pocket. Therefore, the BQCV virion structure provides evidence that capsid-binding antiviral compounds that can prevent the replication of vertebrate picornaviruses may be ineffective against honeybee virus infections.
ER -
SPURNÝ, Radovan, Antonin PRIDAL, Lenka PÁLKOVÁ, Hoa Khanh TRAN KIEM, Joachim R. DE MIRANDA and Pavel PLEVKA. Virion Structure of Black Queen Cell Virus, a Common Honeybee Pathogen. \textit{JOURNAL OF VIROLOGY}. WASHINGTON: AMER SOC MICROBIOLOGY, 2017, vol.~91, No~6, p.~nestránkováno, 14 pp. ISSN~0022-538X. Available from: https://dx.doi.org/10.1128/JVI.02100-16.
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