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@inproceedings{1382530,
author = {Bosáková, Markéta and Třísková, Iveta and Hrbáč, Jan and Trnková, Libuše},
address = {Ústí nad Labem},
booktitle = {XXXVII. Moderní elektrochemické metody},
edition = {1},
editor = {Tomáš Navrátil, Miroslav Fojta, Karolina Schwarzová},
keywords = {guanine; methylguanines; epigenetic modifications; DNA methylation; voltammetry; pencil graphite electrode},
howpublished = {tištěná verze "print"},
language = {eng},
location = {Ústí nad Labem},
isbn = {978-80-905221-5-2},
pages = {19-22},
publisher = {Srsenová Lenka - BEST servis Ústí nad Labem},
title = {The Oxidation Processes of Methylguanines on a Pencil Graphite Electrode},
url = {https://www.bestservis.eu/upload/file/Sbornik_metody17.pdf},
year = {2017}
}
TY - JOUR
ID - 1382530
AU - Bosáková, Markéta - Třísková, Iveta - Hrbáč, Jan - Trnková, Libuše
PY - 2017
TI - The Oxidation Processes of Methylguanines on a Pencil Graphite Electrode
PB - Srsenová Lenka - BEST servis Ústí nad Labem
CY - Ústí nad Labem
SN - 9788090522152
KW - guanine
KW - methylguanines
KW - epigenetic modifications
KW - DNA methylation
KW - voltammetry
KW - pencil graphite electrode
UR - https://www.bestservis.eu/upload/file/Sbornik_metody17.pdf
L2 - https://www.bestservis.eu/upload/file/Sbornik_metody17.pdf
N2 - In the last time, epigenetics is among top disciplines in the field of medicine research because epigenetic modifications of nucleic acids are associated with certain diseases and dysfunctions (e.g. human carcinomas – lung, thyroid, prostate and pancreas tumors-, leukemia, etc.). One of several epigenetic mechanisms that cells use to control gene expression is DNA methylation and one of different methylated guanines, N7- methyl G is recognized as the biomarker of DNA methylation. In the present study, electrochemical oxidation of methylated guanines (1N-, 7N- and 9N- derivatives) is studied to ascertain the relationship between the position of methyl group and the corresponding oxidation behavior. For this reason, the oxidation processes of methylguanines (methylGs) in the dependence on pH and the position of methyl group in the guanine molecule (pyrimidine ring vs. imidazole ring) were investigated. It was found that methylation has significant influence on the oxidation processes of methylGs and their oxidation is easier in the case of CH3- substitution at the pyrimidine ring than at imidazole one. Pencil graphite electrode (PeGE) was used as a working electrode throughout the study, since voltammetric signals of methylGs were poorly defined at conventional carbon electrodes, such as glassy carbon or HOPG.
ER -
BOSÁKOVÁ, Markéta, Iveta TŘÍSKOVÁ, Jan HRBÁČ and Libuše TRNKOVÁ. The Oxidation Processes of Methylguanines on a Pencil Graphite Electrode. In Tomáš Navrátil, Miroslav Fojta, Karolina Schwarzová. \textit{XXXVII. Moderní elektrochemické metody}. 1st ed. Ústí nad Labem: Srsenová Lenka - BEST servis Ústí nad Labem, 2017, p.~19-22. ISBN~978-80-905221-5-2.
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