Detailed Information on Publication Record
2017
The Oxidation Processes of Methylguanines on a Pencil Graphite Electrode
BOSÁKOVÁ, Markéta, Iveta TŘÍSKOVÁ, Jan HRBÁČ and Libuše TRNKOVÁBasic information
Original name
The Oxidation Processes of Methylguanines on a Pencil Graphite Electrode
Authors
BOSÁKOVÁ, Markéta (203 Czech Republic, belonging to the institution), Iveta TŘÍSKOVÁ (203 Czech Republic, belonging to the institution), Jan HRBÁČ (203 Czech Republic, belonging to the institution) and Libuše TRNKOVÁ (203 Czech Republic, guarantor, belonging to the institution)
Edition
1. vyd. Ústí nad Labem, XXXVII. Moderní elektrochemické metody, p. 19-22, 4 pp. 2017
Publisher
Srsenová Lenka - BEST servis Ústí nad Labem
Other information
Language
English
Type of outcome
Stať ve sborníku
Field of Study
10403 Physical chemistry
Country of publisher
Czech Republic
Confidentiality degree
není předmětem státního či obchodního tajemství
Publication form
printed version "print"
References:
RIV identification code
RIV/00216224:14310/17:00096907
Organization unit
Faculty of Science
ISBN
978-80-905221-5-2
UT WoS
000432424300003
Keywords in English
guanine; methylguanines; epigenetic modifications; DNA methylation; voltammetry; pencil graphite electrode
Tags
International impact, Reviewed
Změněno: 23/4/2020 14:43, Mgr. Marie Šípková, DiS.
Abstract
V originále
In the last time, epigenetics is among top disciplines in the field of medicine research because epigenetic modifications of nucleic acids are associated with certain diseases and dysfunctions (e.g. human carcinomas – lung, thyroid, prostate and pancreas tumors-, leukemia, etc.). One of several epigenetic mechanisms that cells use to control gene expression is DNA methylation and one of different methylated guanines, N7- methyl G is recognized as the biomarker of DNA methylation. In the present study, electrochemical oxidation of methylated guanines (1N-, 7N- and 9N- derivatives) is studied to ascertain the relationship between the position of methyl group and the corresponding oxidation behavior. For this reason, the oxidation processes of methylguanines (methylGs) in the dependence on pH and the position of methyl group in the guanine molecule (pyrimidine ring vs. imidazole ring) were investigated. It was found that methylation has significant influence on the oxidation processes of methylGs and their oxidation is easier in the case of CH3- substitution at the pyrimidine ring than at imidazole one. Pencil graphite electrode (PeGE) was used as a working electrode throughout the study, since voltammetric signals of methylGs were poorly defined at conventional carbon electrodes, such as glassy carbon or HOPG.
Links
| MUNI/A/1237/2016, interní kód MU |
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