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@article{1383183,
author = {Gajarský, Martin and Zivkovic, Martina Lenarcic and Stadlbauer, Petr and Pagano, Bruno and Fiala, Radovan and Amato, Jussara and Tomaska, L´ubomir and Šponer, Jiří and Plavec, Janez and Trantírek, Lukáš},
article_location = {WASHINGTON},
article_number = {10},
doi = {http://dx.doi.org/10.1021/jacs.6b10786},
keywords = {G-QUADRUPLEX STRUCTURES; HUMAN TELOMERIC DNA; SINGLE-STRANDED-DNA; G-TRIPLEX; FOLDING PATHWAYS; KINETICS; RECOGNITION; VISUALIZATION; DETERMINANTS; REPEATS},
language = {eng},
issn = {0002-7863},
journal = {Journal of the American Chemical Society},
title = {Structure of a Stable G-Hairpin},
url = {http://pubs.acs.org/doi/abs/10.1021/jacs.6b10786},
volume = {139},
year = {2017}
}
TY - JOUR
ID - 1383183
AU - Gajarský, Martin - Zivkovic, Martina Lenarcic - Stadlbauer, Petr - Pagano, Bruno - Fiala, Radovan - Amato, Jussara - Tomaska, L´ubomir - Šponer, Jiří - Plavec, Janez - Trantírek, Lukáš
PY - 2017
TI - Structure of a Stable G-Hairpin
JF - Journal of the American Chemical Society
VL - 139
IS - 10
SP - 3591-3594
EP - 3591-3594
PB - American Chemical Society
SN - 00027863
KW - G-QUADRUPLEX STRUCTURES
KW - HUMAN TELOMERIC DNA
KW - SINGLE-STRANDED-DNA
KW - G-TRIPLEX
KW - FOLDING PATHWAYS
KW - KINETICS
KW - RECOGNITION
KW - VISUALIZATION
KW - DETERMINANTS
KW - REPEATS
UR - http://pubs.acs.org/doi/abs/10.1021/jacs.6b10786
L2 - http://pubs.acs.org/doi/abs/10.1021/jacs.6b10786
N2 - In this study, we report the first atomic resolution structure of a stable G-hairpin formed by a natively occurring DNA sequence. An 11-nt long G-rich DNA oligonucleotide, 5'-d(GTGTGGGTGTG)-3', corresponding to the most abundant sequence motif in irregular telomeric DNA from Saccharomyces cerevisiae (yeast), is demonstrated to adopt a novel type of mixed parallel/ antiparallel fold-back DNA structure, which is stabilized by dynamic G:G base pairs that transit between NI -carbonyl symmetric and N1-carbonyl, N7-aminobase-pairingarrangements. Although the studied sequence first appears to possess a low capacity for base pairing, it forms a thermodynamically stable structure with a rather complex topology that includes a chain reversal arrangement of the backbone in the center of the continuous G-tract and 3' to -5' stacking of the terminal residues. The structure reveals previously unknown principles of the folding of G rich oligonucleotides that could be applied to the prediction of natural and/or the design of artificial recognition DNA elements. The structure also demonstrates that the folding landscapes of short DNA single strands is much more complex than previously assumed.
ER -
GAJARSKÝ, Martin, Martina Lenarcic ZIVKOVIC, Petr STADLBAUER, Bruno PAGANO, Radovan FIALA, Jussara AMATO, L´ubomir TOMASKA, Jiří ŠPONER, Janez PLAVEC and Lukáš TRANTÍREK. Structure of a Stable G-Hairpin. \textit{Journal of the American Chemical Society}. WASHINGTON: American Chemical Society, 2017, vol.~139, No~10, p.~3591-3594. ISSN~0002-7863. Available from: https://dx.doi.org/10.1021/jacs.6b10786.
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