J 2017

Expression of genes associated with BMP signaling pathway in porcine oocytes before and after IVM - a microarray approach

BUDNA, Joanna, Marta RYBSKA, Sylwia CIESIÓŁKA, Artur BRYJA, Sylwia BORYS et. al.

Basic information

Original name

Expression of genes associated with BMP signaling pathway in porcine oocytes before and after IVM - a microarray approach

Authors

BUDNA, Joanna (616 Poland), Marta RYBSKA (616 Poland), Sylwia CIESIÓŁKA (616 Poland), Artur BRYJA (616 Poland), Sylwia BORYS (616 Poland), Wiesława KRANC (616 Poland), Katarzyna WOJTANOWICZ-MARKIEWICZ (616 Poland), Michal JEŠETA (203 Czech Republic, guarantor, belonging to the institution), Eva SUMELKA (616 Poland), Dorota BUKOWSKA (616 Poland), Paweł ANTOSIK (616 Poland), Klaus P. BRÜSSOW (616 Poland), Małgorzata BRUSKA (616 Poland), Michał NOWICKI (616 Poland), Maciej ZABEL (616 Poland) and Bartosz KEMPISTY (616 Poland)

Edition

Reproductive Biology and Endocrinology, London, BioMed Central, 2017, 1477-7827

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30202 Endocrinology and metabolism

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 2.852

RIV identification code

RIV/00216224:14110/17:00097069

Organization unit

Faculty of Medicine

DOI

UT WoS

000402578900002

Keywords in English

Pig; Oocytes; Microarray; In vitro maturation

Tags

Tags

International impact, Reviewed
Změněno: 20/3/2018 16:11, Soňa Böhmová

Abstract

V originále

Background: The full maturational capability of mammalian oocytes is accompanied by nuclear and cytoplasmic modifications, which are associated with proliferation and differentiation of surrounding cumulus cells. These events are regulated on molecular level by the expression of target genes involved in signal transduction pathways crucial for folliculogenesis and oogenesis. Transforming growth factor beta signaling includes several molecules that are involved in the regulation of oogenesis and embryo growth, including bone morphogenetic protein (BMP). However, the BMP-related gene expression profile in oocytes at different maturational stages requires further investigation. Methods: Oocytes were isolated from pubertal crossbred Landrace gilts follicles, selected with a use of BCB staining test and analyzed before and after in vitro maturation. Gene expression profiles were examined using an Affymetrix microarray approach and validated by RT-qPCR. Database for Annotation, Visualization, and Integrated Discovery (DAVID) software was used for the extraction of the genes belonging to a BMP-signaling pathway ontology group. Results: The assay revealed 12,258 different transcripts in porcine oocytes, among which 379 genes were down-regulated and 40 were up-regulated. The DAVID database indicated a "BMP signaling pathway" ontology group, which was significantly regulated in both groups of oocytes. We discovered five up-regulated genes in oocytes before versus after in vitro maturation (IVM): chordin-like 1 (CHRDL1), follistatin (FST), transforming growth factor-beta receptor-type III (TGF beta R3), decapentaplegic homolog 4 (SMAD4), and inhibitor of DNA binding 1 (ID1). Conclusions: Increased expression of CHRDL1, FST, TGF beta R3, SMAD4, and ID1 transcripts before IVM suggested a subordinate role of the BMP signaling pathway in porcine oocyte maturational competence. Conversely, it is postulated that these genes are involved in early stages of folliculogenesis and oogenesis regulation in pigs, since in oocytes before IVM increased expression was observed.