Characterization of two highly divergent lineages of
exfoliative toxin B-encoding plasmids revealed in impetigo
strains of Staphylococcus aureus

D 2017

Characterization of two highly divergent lineages of exfoliative toxin B-encoding plasmids revealed in impetigo strains of Staphylococcus aureus

BOTKA, Tibor, Vladislava RŮŽIČKOVÁ, Karla SVOBODOVÁ, Roman PANTŮČEK, Petr PETRÁŠ et. al.

Basic information

Original name

Characterization of two highly divergent lineages of exfoliative toxin B-encoding plasmids revealed in impetigo strains of Staphylococcus aureus

Authors

BOTKA, Tibor, Vladislava RŮŽIČKOVÁ, Karla SVOBODOVÁ, Roman PANTŮČEK, Petr PETRÁŠ, Darina ČEJKOVÁ and Jiří DOŠKAŘ

Edition

Germany, EMBO | EMBL Symposium: New Approaches and Concepts in Microbiology, p. 122-122, 2017

Publisher

EMBL Heidelberg, Germany

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

Genetics and molecular biology

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

Publication form

printed version "print"

References:

URL

Organization unit

Faculty of Science

Keywords (in Czech)

ETB plazmid; exfoliativní toxin B; fylogeneze; genomová analýza

Keywords in English

ETB plasmid; exfoliative toxin B; phylogeny; genome analysis

Abstract

V originále

Staphylococcal epidermolytic diseases are caused by S. aureus strains producing exfoliative toxins A and B encoded by mobile genetic elements: plasmids and prophages. We analyzed various etb gene-positive plasmids isolated from the impetigo strains implicated in outbreaks of pemphigus neonatorum in Czech maternity hospitals. Most of the plasmids were related to archetypal plasmid pETBTY4. They shared a same core sequence, but they differed from each other in the content of additional DNA regions such as antibiotic resistance-encoding transposons. The ETB plasmids of this lineage were carried by strains of clonal complex CC121. Some of these strains also harbored exfoliative toxin A-encoding prophages. A novel distinct type of ETB plasmid originated from a prophageless impetigo strain was isolated and characterized in detail. Carrying conjugative cluster genes, as well as new variants of ETB and EDIN genes, it could be regarded as a source of a new lineage of ETB plasmids. Based on our findings, we have designed a helpful detection assay, which facilitates the precise identification of the all described types of ETB plasmids.

Links

QJ1510216, research and development project

Name: Fágová terapie infekcí vyvolaných Staphylococcus aureus v chovech hospodářských zvířat (Acronym: StafyFag)

Investor: Ministry of Agriculture of the CR

Citovat

BOTKA, Tibor, Vladislava RŮŽIČKOVÁ, Karla SVOBODOVÁ, Roman PANTŮČEK, Petr PETRÁŠ, Darina ČEJKOVÁ and Jiří DOŠKAŘ. Characterization of two highly divergent lineages of exfoliative toxin B-encoding plasmids revealed in impetigo strains of Staphylococcus aureus. In Carolina Garcia Sabaté. EMBO | EMBL Symposium: New Approaches and Concepts in Microbiology. Germany: EMBL Heidelberg, Germany, 2017, p. 122.

@inproceedings{1385022,
   author = {Botka, Tibor and Růžičková, Vladislava and Svobodová, Karla and Pantůček, Roman and Petráš, Petr and Čejková, Darina and Doškař, Jiří},
   address = {Germany},
   booktitle = {EMBO | EMBL Symposium: New Approaches and Concepts in Microbiology},
   editor = {Carolina Garcia Sabaté},
   keywords = {ETB plasmid; exfoliative toxin B; phylogeny; genome analysis},
   howpublished = {tištěná verze "print"},
   language = {eng},
   location = {Germany},
   note = {Nepřenášíme do RIV, výsledek má jen 1 stranu, podle metodiky M17+ musí mít stať ve sborníku min. 2 strany.},
   pages = {122-122},
   publisher = {EMBL Heidelberg, Germany},
   title = {Characterization of two highly divergent lineages of exfoliative toxin B-encoding plasmids revealed in impetigo strains of Staphylococcus aureus},
   url = {https://www.embo-embl-symposia.org/symposia/2017/EES17-05/index.html},
   year = {2017}
}

TY  - JOUR
ID  - 1385022
AU  - Botka, Tibor - Růžičková, Vladislava - Svobodová, Karla - Pantůček, Roman - Petráš, Petr - Čejková, Darina - Doškař, Jiří
PY  - 2017
TI  - Characterization of two highly divergent lineages of exfoliative toxin B-encoding plasmids revealed in impetigo strains of Staphylococcus aureus
PB  - EMBL Heidelberg, Germany
CY  - Germany
N1  - Nepřenášíme do RIV, výsledek má jen 1 stranu, podle metodiky M17+ musí mít stať ve sborníku min. 2 strany.
KW  - ETB plasmid
KW  - exfoliative toxin B
KW  - phylogeny
KW  - genome analysis
UR  - https://www.embo-embl-symposia.org/symposia/2017/EES17-05/index.html
N2  - Staphylococcal epidermolytic diseases are caused by S. aureus strains producing exfoliative toxins A and B encoded by mobile genetic elements: plasmids and prophages. We analyzed various etb gene-positive plasmids isolated from the impetigo strains implicated in outbreaks of pemphigus neonatorum in Czech maternity hospitals. Most of the plasmids were related to archetypal plasmid pETBTY4. They shared a same core sequence, but they differed from each other in the content of additional DNA regions such as antibiotic resistance-encoding transposons. The ETB plasmids of this lineage were carried by strains of clonal complex CC121. Some of these strains also harbored exfoliative toxin A-encoding prophages. A novel distinct type of ETB plasmid originated from a prophageless impetigo strain was isolated and characterized in detail. Carrying conjugative cluster genes, as well as new variants of ETB and EDIN genes, it could be regarded as a source of a new lineage of ETB plasmids. Based on our findings, we have designed a helpful detection assay, which facilitates the precise identification of the all described types of ETB plasmids.
ER  -

BOTKA, Tibor, Vladislava RŮŽIČKOVÁ, Karla SVOBODOVÁ, Roman PANTŮČEK, Petr PETRÁŠ, Darina ČEJKOVÁ and Jiří DOŠKAŘ. Characterization of two highly divergent lineages of exfoliative toxin B-encoding plasmids revealed in impetigo strains of Staphylococcus aureus. In Carolina Garcia Sabaté. \textit{EMBO $|$ EMBL Symposium: New Approaches and Concepts in Microbiology}. Germany: EMBL Heidelberg, Germany, 2017, p.~122.

Detailed Information on Publication Record