A new view on the morphology and phylogeny of eugregarines
suggested by the evidence from the gregarine Ancora sagittata
(Leuckart, 1860) Labbe, 1899 (Apicomplexa: Eugregarinida)

J 2017

A new view on the morphology and phylogeny of eugregarines suggested by the evidence from the gregarine Ancora sagittata (Leuckart, 1860) Labbe, 1899 (Apicomplexa: Eugregarinida)

SIMDYANOV, Timur G., Laure GUILLOU, Andrei DIAKIN, Kirill V. MIKHAILOV, Joseph SCHREVEL et. al.

Basic information

Original name

A new view on the morphology and phylogeny of eugregarines suggested by the evidence from the gregarine Ancora sagittata (Leuckart, 1860) Labbe, 1899 (Apicomplexa: Eugregarinida)

Authors

SIMDYANOV, Timur G. (643 Russian Federation), Laure GUILLOU (250 France), Andrei DIAKIN (643 Russian Federation, guarantor, belonging to the institution), Kirill V. MIKHAILOV (643 Russian Federation), Joseph SCHREVEL (250 France) and Vladimir V. ALEOSHIN (643 Russian Federation)

Edition

PeerJ, London, PEERJ INC, 2017, 2167-8359

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 2.118

RIV identification code

RIV/00216224:14310/17:00094845

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.7717/peerj.3354

UT WoS

000402559800002

Keywords in English

Apicomplexa; Marine gregarines; Ultrastructure; SSU and LSU rDNA; Environmental DNA sequences; Phylogeny; Taxonomy

Abstract

V originále

Background: Gregarines are a group of early branching Apicomplexa parasitizing invertebrate animals. Despite their wide distribution and relevance to the understanding the phylogenesis of apicomplexans, gregarines remain understudied: light microscopy data are insufficient for classification, and electron microscopy and molecular data are fragmentary and overlap only partially. Methods: Scanning and transmission electron microscopy, PCR, DNA cloning and sequencing (Sanger and NGS), molecular phylogenetic analyses using ribosomal RNA genes (18S (SSU), 5.8S, and 28S (LSU) ribosomal DNAs (rDNAs)). Results and Discussion: We present the results of an ultrastructural and molecular phylogenetic study on the marine gregarine Ancora sagittata from the polychaete Capitella capitata followed by evolutionary and taxonomic synthesis of the morphological and molecular phylogenetic evidence on eugregarines. The ultrastructure of Ancora sagittata generally corresponds to that of other eugregarines, but reveals some differences in epicytic folds (crests) and attachment apparatus to gregarines in the family Lecudinidae, where Ancora sagittata has been classified. Molecular phylogenetic trees based on SSU (18S) rDNA reveal several robust clades (superfamilies) of eugregarines, including Ancoroidea superfam. nov., which comprises two families (Ancoridae fam. nov. and Polyplicariidae) and branches separately from the Lecudinidae; thus, all representatives of Ancoroidea are here officially removed from the Lecudinidae. Analysis of sequence data also points to possible cryptic species within Ancora sagittata and the inclusion of numerous environmental sequences from anoxic habitats within the Ancoroidea. LSU (28S) rDNA phylogenies, unlike the analysis of SSU rDNA alone, recover a well-supported monophyly of the gregarines involved (eugregarines), although this conclusion is currently limited by sparse taxon sampling and the presence of fast-evolving sequences in some species. Comparative morphological analyses of gregarine teguments and attachment organelles lead us to revise their terminology. The terms "longitudinal folds" and "mucron" are restricted to archigregarines, whereas the terms "epicystic crests" and "epimerite" are proposed to describe the candidate synapomorphies of eugregarines, which, consequently, are considered as a monophyletic group. Abolishing the suborders Aseptata and Septata, incorporating neogregarines into the Eugregarinida, and treating the major molecular phylogenetic lineages of eugregarines as superfamilies appear as the best way of reconciling recent morphological and molecular evidence. Accordingly, the diagnosis of the order Eugregarinida Léger, 1900 is updated.

Links

GBP505/12/G112, research and development project

Name: ECIP - Evropské centrum ichtyoparazitologie

Investor: Czech Science Foundation

Citovat

SIMDYANOV, Timur G., Laure GUILLOU, Andrei DIAKIN, Kirill V. MIKHAILOV, Joseph SCHREVEL and Vladimir V. ALEOSHIN. A new view on the morphology and phylogeny of eugregarines suggested by the evidence from the gregarine Ancora sagittata (Leuckart, 1860) Labbe, 1899 (Apicomplexa: Eugregarinida). PeerJ. London: PEERJ INC, 2017, Neuveden, MAY, p. nestránkováno, 46 pp. ISSN 2167-8359. Available from: https://dx.doi.org/10.7717/peerj.3354.

@article{1385950,
   author = {Simdyanov, Timur G. and Guillou, Laure and Diakin, Andrei and Mikhailov, Kirill V. and Schrevel, Joseph and Aleoshin, Vladimir V.},
   article_location = {London},
   article_number = {MAY},
   doi = {http://dx.doi.org/10.7717/peerj.3354},
   keywords = {Apicomplexa; Marine gregarines; Ultrastructure; SSU and LSU rDNA; Environmental DNA sequences; Phylogeny; Taxonomy},
   language = {eng},
   issn = {2167-8359},
   journal = {PeerJ},
   title = {A new view on the morphology and phylogeny of eugregarines suggested by the evidence from the gregarine Ancora sagittata (Leuckart, 1860) Labbe, 1899 (Apicomplexa: Eugregarinida)},
   volume = {Neuveden},
   year = {2017}
}

TY  - JOUR
ID  - 1385950
AU  - Simdyanov, Timur G. - Guillou, Laure - Diakin, Andrei - Mikhailov, Kirill V. - Schrevel, Joseph - Aleoshin, Vladimir V.
PY  - 2017
TI  - A new view on the morphology and phylogeny of eugregarines suggested by the evidence from the gregarine Ancora sagittata (Leuckart, 1860) Labbe, 1899 (Apicomplexa: Eugregarinida)
JF  - PeerJ
VL  - Neuveden
IS  - MAY
SP  - nestránkováno
EP  - nestránkováno
PB  - PEERJ INC
SN  - 21678359
KW  - Apicomplexa
KW  - Marine gregarines
KW  - Ultrastructure
KW  - SSU and LSU rDNA
KW  - Environmental DNA sequences
KW  - Phylogeny
KW  - Taxonomy
N2  - Background: Gregarines are a group of early branching Apicomplexa parasitizing invertebrate animals. Despite their wide distribution and relevance to the understanding the phylogenesis of apicomplexans, gregarines remain understudied: light microscopy data are insufficient for classification, and electron microscopy and molecular data are fragmentary and overlap only partially. Methods: Scanning and transmission electron microscopy, PCR, DNA cloning and sequencing (Sanger and NGS), molecular phylogenetic analyses using ribosomal RNA genes (18S (SSU), 5.8S, and 28S (LSU) ribosomal DNAs (rDNAs)). Results and Discussion: We present the results of an ultrastructural and molecular phylogenetic study on the marine gregarine Ancora sagittata from the polychaete Capitella capitata followed by evolutionary and taxonomic synthesis of the morphological and molecular phylogenetic evidence on eugregarines. The ultrastructure of Ancora sagittata generally corresponds to that of other eugregarines, but reveals some differences in epicytic folds (crests) and attachment apparatus to gregarines in the family Lecudinidae, where Ancora sagittata has been classified. Molecular phylogenetic trees based on SSU (18S) rDNA reveal several robust clades (superfamilies) of eugregarines, including Ancoroidea superfam. nov., which comprises two families (Ancoridae fam. nov. and Polyplicariidae) and branches separately from the Lecudinidae; thus, all representatives of Ancoroidea are here officially removed from the Lecudinidae. Analysis of sequence data also points to possible cryptic species within Ancora sagittata and the inclusion of numerous environmental sequences from anoxic habitats within the Ancoroidea. LSU (28S) rDNA phylogenies, unlike the analysis of SSU rDNA alone, recover a well-supported monophyly of the gregarines involved (eugregarines), although this conclusion is currently limited by sparse taxon sampling and the presence of fast-evolving sequences in some species. Comparative morphological analyses of gregarine teguments and attachment organelles lead us to revise their terminology. The terms "longitudinal folds" and "mucron" are restricted to archigregarines, whereas the terms "epicystic crests" and "epimerite" are proposed to describe the candidate synapomorphies of eugregarines, which, consequently, are considered as a monophyletic group. Abolishing the suborders Aseptata and Septata, incorporating neogregarines into the Eugregarinida, and treating the major molecular phylogenetic lineages of eugregarines as superfamilies appear as the best way of reconciling recent morphological and molecular evidence. Accordingly, the diagnosis of the order Eugregarinida Léger, 1900 is updated.
ER  -

SIMDYANOV, Timur G., Laure GUILLOU, Andrei DIAKIN, Kirill V. MIKHAILOV, Joseph SCHREVEL and Vladimir V. ALEOSHIN. A new view on the morphology and phylogeny of eugregarines suggested by the evidence from the gregarine Ancora sagittata (Leuckart, 1860) Labbe, 1899 (Apicomplexa: Eugregarinida). \textit{PeerJ}. London: PEERJ INC, 2017, Neuveden, MAY, p.~nestránkováno, 46 pp. ISSN~2167-8359. Available from: https://dx.doi.org/10.7717/peerj.3354.

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