The main objective of this cross-cultural comparative study was to explore the role of perceived hope and dispositional hope in life satisfaction in two samples, Czech and Indian young adults. The secondary objective was to investigate interrelationships between hope, spirituality, meaning of life, depression, quality of relationships and life satisfaction and to reveal potential differences between the two national samples in predictors of life satisfaction. The research sample consisted of 111 Czech and 129 Indian respondents aged 18 to 29 years. A set of questionnaires was used for data collection. Basic demographic data were also obtained. Non-random convenience sampling was used for participant recruitment. The data were analysed using the IBM SPSS 24 software. Comparative analysis has shown that Czech respondents scored significantly higher in perceived hope, life satisfaction, spirituality, meaning of life, and positive relationships, while Indian respondents have reported higher depression. The results of correlation analysis have revealed cultural differences with regard to two concepts of hope: In Czech sample perceived hope has been found to be in a tighter correlation with the investigated variables than dispositional hope while in Indian sample it was dispositional hope that has shown tighter correlations with other variables.Regression analysis revealed detailed interrelationships between measured variables: In Czech sample, the main independent predictors of life satisfaction were Depression and Perceived Hope, while life satisfaction of Indian sample was mainly affected by Dispositional Hope, Meaningfulness, and Spirituality. The regression model explained 57 % of variance of life satisfaction in Czech sample and 54 % of variance of life satisfaction in Indian sample.