Hematopoietic developmental potential of human pluripotent stem cell lines is accompanied by the morphology of embryoid bodies and the expression of endodermal and hematopoietic markers

TESAŘOVÁ, Lenka, Pavel ŠIMARA, Stanislav STEJSKAL and Irena KRONTORÁD KOUTNÁ. Hematopoietic developmental potential of human pluripotent stem cell lines is accompanied by the morphology of embryoid bodies and the expression of endodermal and hematopoietic markers. Cellular Reprogramming. 2017, vol. 19, No 4, p. 270-284. ISSN 2152-4971. Available from: https://dx.doi.org/10.1089/cell.2016.0042.

Basic information

• Original name: Hematopoietic developmental potential of human pluripotent stem cell lines is accompanied by the morphology of embryoid bodies and the expression of endodermal and hematopoietic markers
• Authors: TESAŘOVÁ, Lenka (203 Czech Republic, belonging to the institution), Pavel ŠIMARA (203 Czech Republic, belonging to the institution), Stanislav STEJSKAL (203 Czech Republic, belonging to the institution) and Irena KRONTORÁD KOUTNÁ (203 Czech Republic, guarantor, belonging to the institution).
• Edition: Cellular Reprogramming, 2017, 2152-4971.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10603 Genetics and heredity
• Country of publisher: United Kingdom of Great Britain and Northern Ireland
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• Impact factor: Impact factor: 1.430
• RIV identification code: RIV/00216224:14330/17:00094855
• Organization unit: Faculty of Informatics
• Doi: http://dx.doi.org/10.1089/cell.2016.0042
• UT WoS: 000406526900007
• Keywords in English: pluripotent stem cells; hematopoietic differentiation; embryoid bodies; cytokines; hematopoietic stem cells
• Tags: cbia-web

Abstract

The potential clinical applications of hematopoietic stem cells derived from human pluripotent stem cells (hPSCs) are limited by the difficulty of recapitulating embryoid haematopoiesis and by the unknown differentiation potential of hPSC lines. To evaluate their hematopoietic developmental potential, available hPSC lines were differentiated via an embryoid body (EB) suspension culture in serum-free medium supplemented with three different cytokine mixes. The hPSC differentiation status was investigated by the flow cytometry expression profiles of cell surface molecules, and the gene expression of pluripotency and differentiation markers over time was evaluated by qRT-PCR. hPSC lines differed in several aspects of the differentiation process, including the absolute yield of hematopoietic progenitors, the proportion of hematopoietic progenitor populations and the effect of various cytokine mixes. The ability to generate hematopoietic progenitors was then associated with the morphology of the developing EBs and with the expression of the endodermal markers AFP and SOX17 and the hematopoietic transcription factor RUNX1. These findings deepen the knowledge about the hematopoietic propensity of hPSCs and identify its variability as an aspect that must be taken into account before the usage of hPSC-derived HSCs in downstream applications.

Links

• GBP302/12/G157, research and development project: Name: Dynamika a organizace chromosomů během buněčného cyklu a při diferenciaci v normě a patologii

Investor: Czech Science Foundation

• LM2015090, research and development project: Name: Český národní uzel Evropské sítě infrastruktur klinického výzkumu (Acronym: CZECRIN)

Investor: Ministry of Education, Youth and Sports of the CR