J 2017

Hematopoietic developmental potential of human pluripotent stem cell lines is accompanied by the morphology of embryoid bodies and the expression of endodermal and hematopoietic markers

TESAŘOVÁ, Lenka, Pavel ŠIMARA, Stanislav STEJSKAL and Irena KRONTORÁD KOUTNÁ

Basic information

Original name

Hematopoietic developmental potential of human pluripotent stem cell lines is accompanied by the morphology of embryoid bodies and the expression of endodermal and hematopoietic markers

Authors

TESAŘOVÁ, Lenka (203 Czech Republic, belonging to the institution), Pavel ŠIMARA (203 Czech Republic, belonging to the institution), Stanislav STEJSKAL (203 Czech Republic, belonging to the institution) and Irena KRONTORÁD KOUTNÁ (203 Czech Republic, guarantor, belonging to the institution)

Edition

Cellular Reprogramming, 2017, 2152-4971

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10603 Genetics and heredity

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 1.430

RIV identification code

RIV/00216224:14330/17:00094855

Organization unit

Faculty of Informatics

DOI

UT WoS

000406526900007

Keywords in English

pluripotent stem cells; hematopoietic differentiation; embryoid bodies; cytokines; hematopoietic stem cells

Tags

Změněno: 27/1/2021 11:57, Mgr. Tereza Miškechová

Abstract

V originále

The potential clinical applications of hematopoietic stem cells derived from human pluripotent stem cells (hPSCs) are limited by the difficulty of recapitulating embryoid haematopoiesis and by the unknown differentiation potential of hPSC lines. To evaluate their hematopoietic developmental potential, available hPSC lines were differentiated via an embryoid body (EB) suspension culture in serum-free medium supplemented with three different cytokine mixes. The hPSC differentiation status was investigated by the flow cytometry expression profiles of cell surface molecules, and the gene expression of pluripotency and differentiation markers over time was evaluated by qRT-PCR. hPSC lines differed in several aspects of the differentiation process, including the absolute yield of hematopoietic progenitors, the proportion of hematopoietic progenitor populations and the effect of various cytokine mixes. The ability to generate hematopoietic progenitors was then associated with the morphology of the developing EBs and with the expression of the endodermal markers AFP and SOX17 and the hematopoietic transcription factor RUNX1. These findings deepen the knowledge about the hematopoietic propensity of hPSCs and identify its variability as an aspect that must be taken into account before the usage of hPSC-derived HSCs in downstream applications.

Links

GBP302/12/G157, research and development project
Name: Dynamika a organizace chromosomů během buněčného cyklu a při diferenciaci v normě a patologii
Investor: Czech Science Foundation
LM2015090, research and development project
Name: Český národní uzel Evropské sítě infrastruktur klinického výzkumu (Acronym: CZECRIN)
Investor: Ministry of Education, Youth and Sports of the CR