A Comprehensive In Vitro Comparison of Preparation Techniques
for Fat Grafting

J 2017

A Comprehensive In Vitro Comparison of Preparation Techniques for Fat Grafting

STREIT, Libor, Josef JAROŠ, Veronika SEDLÁKOVÁ, Miroslava SEDLÁČKOVÁ, Luboš DRAŽAN et. al.

Basic information

Original name

A Comprehensive In Vitro Comparison of Preparation Techniques for Fat Grafting

Authors

STREIT, Libor (203 Czech Republic, guarantor, belonging to the institution), Josef JAROŠ (203 Czech Republic, belonging to the institution), Veronika SEDLÁKOVÁ (203 Czech Republic, belonging to the institution), Miroslava SEDLÁČKOVÁ (203 Czech Republic, belonging to the institution), Luboš DRAŽAN (203 Czech Republic), Michal SVOBODA (203 Czech Republic, belonging to the institution), Jakub POSPÍŠIL (203 Czech Republic, belonging to the institution), Tomáš VÝŠKA (203 Czech Republic), Jiří VESELÝ (203 Czech Republic) and Aleš HAMPL (203 Czech Republic, belonging to the institution)

Edition

PLASTIC AND RECONSTRUCTIVE SURGERY, PHILADELPHIA, LIPPINCOTT WILLIAMS & WILKINS, 2017, 0032-1052

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30212 Surgery

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 3.621

RIV identification code

RIV/00216224:14110/17:00094856

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1097/PRS.0000000000003124

UT WoS

000395678200029

Keywords in English

reconstructive surgery

Abstract

V originále

Background: Lipomodeling is a technique that uses the patient's own fat for tissue regeneration and augmentation. The extent of regenerative effect is reported to be determined by the numbers of adipose-derived stem cells and the viability of cells in processed adipose tissue which, together with other factors, influence the degree of graft retention. This study addresses whether differences exist in properties of fat graft obtained by three commonly used techniques. Methods: Adipose tissue harvested from the hypogastric regions of 14 patients was processed by decantation, centrifugation, and membrane-based tissue filtration. The morphology of each preparation was assessed by electron microscopy and overall cell viability was assessed by live/dead assay. The number of adiposederived stem cells was determined and their stem cell character was assessed by the presence of cell surface molecules (i.e., CD105, CD90, CD31, and CD45) and by their capacity to differentiate into adipogenic and osteogenic lineages. Results: First, morphologies of processed fat samples obtained by individual procedures differed, but no preparation caused obvious damage to cellular or acellular components. Second, although the highest numbers of adiposederived stem cells were contained in the upper fraction of centrifuged lipoaspirates, the difference between preparations was marginal. Third, the maximal concentration of adipose fraction (removal of watery component) of lipoaspirate was achieved by membrane-based tissue filtration. Finally, no significant differences in overall viability were detected. Conclusions: Properties of processed lipoaspirate were influenced by the preparation procedure. However, the differences were not dramatic; both centrifugation and membrane-based filtration are methods of choice whose selection depends on other criteria (e.g., practicality) for individual surgical settings.

Links

ED1.1.00/02.0068, research and development project

Name: CEITEC - central european institute of technology

EE2.3.20.0185, research and development project

Name: Centrum analýz a modelování tkání a orgánů

GA16-02702S, research and development project

Name: Materiály s nanostrukturovaným povrchem pro biomediciální aplikace

Investor: Czech Science Foundation

LD15144, research and development project

Name: Buněčné a nebuněčné základy pro regeneraci kostí a zubů (Acronym: TissueENG)

Investor: Ministry of Education, Youth and Sports of the CR

MUNI/A/1558/2014, interní kód MU

Name: Zdroje pro tkáňové inženýrství 5 (Acronym: TissueEng5)

Investor: Masaryk University, Category A

Citovat

STREIT, Libor, Josef JAROŠ, Veronika SEDLÁKOVÁ, Miroslava SEDLÁČKOVÁ, Luboš DRAŽAN, Michal SVOBODA, Jakub POSPÍŠIL, Tomáš VÝŠKA, Jiří VESELÝ and Aleš HAMPL. A Comprehensive In Vitro Comparison of Preparation Techniques for Fat Grafting. PLASTIC AND RECONSTRUCTIVE SURGERY. PHILADELPHIA: LIPPINCOTT WILLIAMS & WILKINS, 2017, vol. 139, No 3, p. "670E"-"682E", 13 pp. ISSN 0032-1052. Available from: https://dx.doi.org/10.1097/PRS.0000000000003124.

@article{1386073,
   author = {Streit, Libor and Jaroš, Josef and Sedláková, Veronika and Sedláčková, Miroslava and Dražan, Luboš and Svoboda, Michal and Pospíšil, Jakub and Výška, Tomáš and Veselý, Jiří and Hampl, Aleš},
   article_location = {PHILADELPHIA},
   article_number = {3},
   doi = {http://dx.doi.org/10.1097/PRS.0000000000003124},
   keywords = {reconstructive surgery},
   language = {eng},
   issn = {0032-1052},
   journal = {PLASTIC AND RECONSTRUCTIVE SURGERY},
   title = {A Comprehensive In Vitro Comparison of Preparation Techniques for Fat Grafting},
   volume = {139},
   year = {2017}
}

TY  - JOUR
ID  - 1386073
AU  - Streit, Libor - Jaroš, Josef - Sedláková, Veronika - Sedláčková, Miroslava - Dražan, Luboš - Svoboda, Michal - Pospíšil, Jakub - Výška, Tomáš - Veselý, Jiří - Hampl, Aleš
PY  - 2017
TI  - A Comprehensive In Vitro Comparison of Preparation Techniques for Fat Grafting
JF  - PLASTIC AND RECONSTRUCTIVE SURGERY
VL  - 139
IS  - 3
SP  - "670E"-"682E"
EP  - "670E"-"682E"
PB  - LIPPINCOTT WILLIAMS & WILKINS
SN  - 00321052
KW  - reconstructive surgery
N2  - Background: Lipomodeling is a technique that uses the patient's own fat for tissue regeneration and augmentation. The extent of regenerative effect is reported to be determined by the numbers of adipose-derived stem cells and the viability of cells in processed adipose tissue which, together with other factors, influence the degree of graft retention. This study addresses whether differences exist in properties of fat graft obtained by three commonly used techniques. Methods: Adipose tissue harvested from the hypogastric regions of 14 patients was processed by decantation, centrifugation, and membrane-based tissue filtration. The morphology of each preparation was assessed by electron microscopy and overall cell viability was assessed by live/dead assay. The number of adiposederived stem cells was determined and their stem cell character was assessed by the presence of cell surface molecules (i.e., CD105, CD90, CD31, and CD45) and by their capacity to differentiate into adipogenic and osteogenic lineages. Results: First, morphologies of processed fat samples obtained by individual procedures differed, but no preparation caused obvious damage to cellular or acellular components. Second, although the highest numbers of adiposederived stem cells were contained in the upper fraction of centrifuged lipoaspirates, the difference between preparations was marginal. Third, the maximal concentration of adipose fraction (removal of watery component) of lipoaspirate was achieved by membrane-based tissue filtration. Finally, no significant differences in overall viability were detected. Conclusions: Properties of processed lipoaspirate were influenced by the preparation procedure. However, the differences were not dramatic; both centrifugation and membrane-based filtration are methods of choice whose selection depends on other criteria (e.g., practicality) for individual surgical settings.
ER  -

STREIT, Libor, Josef JAROŠ, Veronika SEDLÁKOVÁ, Miroslava SEDLÁČKOVÁ, Luboš DRAŽAN, Michal SVOBODA, Jakub POSPÍŠIL, Tomáš VÝŠKA, Jiří VESELÝ and Aleš HAMPL. A Comprehensive In Vitro Comparison of Preparation Techniques for Fat Grafting. \textit{PLASTIC AND RECONSTRUCTIVE SURGERY}. PHILADELPHIA: LIPPINCOTT WILLIAMS \&{} WILKINS, 2017, vol.~139, No~3, p.~''670E''-''682E'', 13 pp. ISSN~0032-1052. Available from: https://dx.doi.org/10.1097/PRS.0000000000003124.

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