LC coupled to ESI, MALDI and ICP MS - A multiple hyphenation
for metalloproteomic studies

J 2017

LC coupled to ESI, MALDI and ICP MS - A multiple hyphenation for metalloproteomic studies

COUFALÍKOVÁ, Kateřina, Iva BENEŠOVÁ, Tomáš VACULOVIČ, Viktor KANICKÝ, Jan PREISLER et. al.

Basic information

Original name

LC coupled to ESI, MALDI and ICP MS - A multiple hyphenation for metalloproteomic studies

Authors

COUFALÍKOVÁ, Kateřina (203 Czech Republic, belonging to the institution), Iva BENEŠOVÁ (203 Czech Republic, belonging to the institution), Tomáš VACULOVIČ (203 Czech Republic, belonging to the institution), Viktor KANICKÝ (203 Czech Republic, belonging to the institution) and Jan PREISLER (203 Czech Republic, guarantor, belonging to the institution)

Edition

Analytica Chimica Acta, Amsterdam, Elsevier Science BV, 2017, 0003-2670

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10406 Analytical chemistry

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 5.123

RIV identification code

RIV/00216224:14310/17:00094859

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1016/j.aca.2017.03.016

UT WoS

000398436800006

Keywords in English

Matrix-assisted laser desorption/ionization; mass spectrometry; Inductively coupled plasma mass; spectrometry; Electrospray ionization mass spectrometry; Liquid chromatography; Metallothionein isoforms; Metal complexes

Abstract

V originále

A new multiple detection arrangement for liquid chromatography (LC) that supplements conventional electrospray ionization (ESI) mass spectrometry (MS) detection with two complementary detection techniques, matrix-assisted laser desorption/ionization (MALDI) MS and substrate-assisted laser desorption inductively coupled plasma (SALD ICP) MS has been developed. The combination of the molecular and elemental detectors in a single separation run is accomplished by utilizing a commercial MALDI target made of conductive plastic. The proposed platform provides a number of benefits in today's metalloproteomic applications, which are demonstrated by analysis of a metallothionein mixture. To maintain metallothionein complexes, separation is carried out at a neutral pH. The effluent is split; a major portion is directed to ESI MS while the remaining 1.8% fraction is deposited onto a plastic MALDI target. Dried droplets are overlaid with MALDI matrix and analysed consecutively by MALDI MS and SALD ICP MS. In the ESI MS spectra, the MT isoform complexes with metals and their stoichiometry are determined; the apoforms are revealed in the MALDI MS spectra. Quantitative determination of metallothionein isoforms is performed via determination of metals in the complexes of the individual protein isoforms using SALD ICP MS. (C) 2017 Elsevier B.V. All rights reserved.

Links

GA15-05387S, research and development project

Name: Laserově desorpční hmotnostní spektrometrie pro analýzu molekul a nanočástic

Investor: Czech Science Foundation

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

COUFALÍKOVÁ, Kateřina, Iva BENEŠOVÁ, Tomáš VACULOVIČ, Viktor KANICKÝ and Jan PREISLER. LC coupled to ESI, MALDI and ICP MS - A multiple hyphenation for metalloproteomic studies. Analytica Chimica Acta. Amsterdam: Elsevier Science BV, 2017, vol. 968, May, p. 58-65. ISSN 0003-2670. Available from: https://dx.doi.org/10.1016/j.aca.2017.03.016.

@article{1386370,
   author = {Coufalíková, Kateřina and Benešová, Iva and Vaculovič, Tomáš and Kanický, Viktor and Preisler, Jan},
   article_location = {Amsterdam},
   article_number = {May},
   doi = {http://dx.doi.org/10.1016/j.aca.2017.03.016},
   keywords = {Matrix-assisted laser desorption/ionization; mass spectrometry; Inductively coupled plasma mass; spectrometry; Electrospray ionization mass spectrometry; Liquid chromatography; Metallothionein isoforms; Metal complexes},
   language = {eng},
   issn = {0003-2670},
   journal = {Analytica Chimica Acta},
   title = {LC coupled to ESI, MALDI and ICP MS - A multiple hyphenation for metalloproteomic studies},
   volume = {968},
   year = {2017}
}

TY  - JOUR
ID  - 1386370
AU  - Coufalíková, Kateřina - Benešová, Iva - Vaculovič, Tomáš - Kanický, Viktor - Preisler, Jan
PY  - 2017
TI  - LC coupled to ESI, MALDI and ICP MS - A multiple hyphenation for metalloproteomic studies
JF  - Analytica Chimica Acta
VL  - 968
IS  - May
SP  - 58-65
EP  - 58-65
PB  - Elsevier Science BV
SN  - 00032670
KW  - Matrix-assisted laser desorption/ionization
KW  - mass spectrometry
KW  - Inductively coupled plasma mass
KW  - spectrometry
KW  - Electrospray ionization mass spectrometry
KW  - Liquid chromatography
KW  - Metallothionein isoforms
KW  - Metal complexes
N2  - A new multiple detection arrangement for liquid chromatography (LC) that supplements conventional electrospray ionization (ESI) mass spectrometry (MS) detection with two complementary detection techniques, matrix-assisted laser desorption/ionization (MALDI) MS and substrate-assisted laser desorption inductively coupled plasma (SALD ICP) MS has been developed. The combination of the molecular and elemental detectors in a single separation run is accomplished by utilizing a commercial MALDI target made of conductive plastic. The proposed platform provides a number of benefits in today's metalloproteomic applications, which are demonstrated by analysis of a metallothionein mixture. To maintain metallothionein complexes, separation is carried out at a neutral pH. The effluent is split; a major portion is directed to ESI MS while the remaining 1.8% fraction is deposited onto a plastic MALDI target. Dried droplets are overlaid with MALDI matrix and analysed consecutively by MALDI MS and SALD ICP MS. In the ESI MS spectra, the MT isoform complexes with metals and their stoichiometry are determined; the apoforms are revealed in the MALDI MS spectra. Quantitative determination of metallothionein isoforms is performed via determination of metals in the complexes of the individual protein isoforms using SALD ICP MS. (C) 2017 Elsevier B.V. All rights reserved.
ER  -

COUFALÍKOVÁ, Kateřina, Iva BENEŠOVÁ, Tomáš VACULOVIČ, Viktor KANICKÝ and Jan PREISLER. LC coupled to ESI, MALDI and ICP MS - A multiple hyphenation for metalloproteomic studies. \textit{Analytica Chimica Acta}. Amsterdam: Elsevier Science BV, 2017, vol.~968, May, p.~58-65. ISSN~0003-2670. Available from: https://dx.doi.org/10.1016/j.aca.2017.03.016.

Detailed Information on Publication Record